Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,918,745	A→G	G371G (GGT→GGC)	atpA ←	F1 sector of membrane‑bound ATP synthase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,918,745	0	A	G	100.0%	86.4 / NA	24	G371G (GGT→GGC)	atpA	F1 sector of membrane‑bound ATP synthase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/11);  total (13/11)

GCAAACTGAGAGAACGCTGCCAGTTCACGATACTGTGCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATA  >  NC_000913/3918629‑3918871                                                                                                                     |                                                                                                                               gCAAACTGAGAGAACGCTGCCAGTTCACGATACTGTGCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGaa                                                                                                          <  1:68413/139‑1 (MQ=255)    aaCTGAGAGAACGCTGCCAGTTCACGATACTGTGCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATAc                                                                                                       <  1:378143/139‑1 (MQ=255)         agagaACGCTGCCAGTTCACGATACTGTGCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCg                                                                                                  <  2:480438/139‑1 (MQ=255)                 cTGCCAGTTCACGATACTGTGCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAg                                                                                          >  1:649361/1‑139 (MQ=255)                                  tgtgCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAg                                                                              <  1:201390/134‑1 (MQ=255)                                  tgtgCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAg                                                                              >  2:201390/1‑134 (MQ=255)                                    tgCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATc                                                                       >  1:272678/1‑139 (MQ=255)                                    tgCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTCTTCTGTGCTGCACCACCAACCCGGGATACGGACATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATc                                                                       >  1:132945/1‑139 (MQ=255)                                         tagCGGTACGGATACCACCGGACAGCCCTTTCATGAGCTTTGTCTGTCATGCACCCGCAACACGGGATACGGAAATGCCCGCGTTGACCGCAGGACGAATACCGGCGTCGTGCAGGTTGGTTTCCAGGAAGATCTAAcc                                                                  >  1:318171/2‑139 (MQ=255)                                         gagCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGc                                                                                                                  >  1:117628/1‑91 (MQ=255)                                         gagCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGc                                                                                                                  <  2:117628/91‑1 (MQ=255)                                         gagCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGAcc                                                                  >  2:195762/1‑139 (MQ=255)                                                  ggATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAAt                                                         <  1:494093/139‑1 (MQ=255)                                                    aTACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCg                                                                                                  <  2:356265/96‑1 (MQ=255)                                                    aTACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCg                                                                                                  >  1:356265/1‑96 (MQ=255)                                                         accGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCAt                                                                >  2:9032/1‑125 (MQ=255)                                                         accGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCAt                                                                <  1:9032/125‑1 (MQ=255)                                                         accGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGAtt                                                  >  2:498003/1‑139 (MQ=255)                                                                          tGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGgaacgaa                                 <  2:649361/139‑1 (MQ=255)                                                                                       tgctgcACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCa                    >  1:654890/1‑139 (MQ=255)                                                                                             accaccAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGcc              <  1:524984/139‑1 (MQ=255)                                                                                                 ccaACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCAt                                                                <  2:502936/85‑1 (MQ=255)                                                                                                 ccaACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCAt                                                                >  1:502936/1‑85 (MQ=255)                                                                                                 ccaACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGGTTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAg          >  1:504224/1‑139 (MQ=255)                                                                                                         ggATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATa  >  1:402084/1‑139 (MQ=255)                                                                                                                     |                                                                                                                               GCAAACTGAGAGAACGCTGCCAGTTCACGATACTGTGCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATA  >  NC_000913/3918629‑3918871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑