Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	398,108	G→A	V79V (GTG→GTA)	yaiW →	microcin Bac7 uptake protein, outer membrane surface‑exposed lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	398,108	0	G	A	100.0%	94.6 / NA	28	V79V (GTG→GTA)	yaiW	microcin Bac7 uptake protein, outer membrane surface‑exposed lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/16);  total (12/16)

CGTCGTGCGGCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGACCGTCGTGCTGAACGGATGCACATTCCTGCTTTT  >  NC_000913/397991‑398225                                                                                                                      |                                                                                                                      cgtcgtGCGGCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCgg                                                                                                                >  1:168549/1‑125 (MQ=255) cgtcgtGCGGCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCgg                                                                                                                <  2:168549/125‑1 (MQ=255) cgtcgtGCGGCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAg                                                                                                  >  2:475828/1‑139 (MQ=255) cgtcgtGCGGCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAg                                                                                                  >  1:501965/1‑139 (MQ=255) cgtcgtGCGGCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAg                                                                                                  >  2:12156/1‑139 (MQ=255)    cgtGCGGCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCa                                                                                               <  2:456144/139‑1 (MQ=255)      tGCGGCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAgg                                                                                                    <  2:465966/132‑1 (MQ=255)      tGCGGCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAgg                                                                                                    >  1:465966/1‑132 (MQ=255)         ggCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCAAATTa                                                                                          <  2:369327/139‑1 (MQ=255)          gCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCAAATTAt                                                                                         >  1:104931/1‑139 (MQ=255)              aagaTGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCAAATTATCAgg                                                                                     <  2:501965/139‑1 (MQ=255)                            cGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGtt                                                                       >  1:632635/1‑139 (MQ=255)                              tGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTcc                                                                     <  2:632635/139‑1 (MQ=255)                                aaaGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCgg                                                                   <  2:104931/139‑1 (MQ=255)                                      cgcgATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCAAATTAt                                                                                         >  2:203841/1‑111 (MQ=255)                                      cgcgATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCAAATTAt                                                                                         <  1:203841/111‑1 (MQ=255)                                      cgcgATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTaa                                                             >  2:168865/1‑139 (MQ=255)                                                     aaaGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGc                                              <  1:168865/139‑1 (MQ=255)                                                     aaaGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGc                                              <  1:12156/139‑1 (MQ=255)                                                                          gAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGACCGTCGTGCTg                         <  1:649796/139‑1 (MQ=255)                                                                                cAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCg                                                                                                                 >  1:541454/1‑45 (MQ=255)                                                                                cAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCg                                                                                                                 <  2:541454/45‑1 (MQ=255)                                                                                         gCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCg                                                    >  2:331011/1‑97 (MQ=255)                                                                                         gCACCGACGCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCg                                                    <  1:331011/97‑1 (MQ=255)                                                                                                 gCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGACCGTCGTGCTGAACGGATGCACATTCCTGCtttt  <  1:652531/139‑1 (MQ=255)                                                                                                 gCTGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGACCGTCGTGCTGAACGGATGCACATTCCTGCtttt  <  2:293700/139‑1 (MQ=255)                                                                                                   tGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGc                                                                           >  2:112571/1‑64 (MQ=255)                                                                                                   tGGAAAACGTCTGCTCGGTACTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGc                                                                           <  1:112571/64‑1 (MQ=255)                                                                                                                      |                                                                                                                      CGTCGTGCGGCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTCGCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTGGCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAGATCGCCTGGCAAGAGATTGACCGTCGTGCTGAACGGATGCACATTCCTGCTTTT  >  NC_000913/397991‑398225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑