Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,936,381	C→T	T35M (ACG→ATG)	rbsB →	D‑ribose ABC transporter periplasmic binding protein, ribose chemotaxis receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,936,381	0	C	T	100.0%	62.3 / NA	19	T35M (ACG→ATG)	rbsB	D‑ribose ABC transporter periplasmic binding protein, ribose chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/11);  total (8/11)

AGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCG  >  NC_000913/3936246‑3936481                                                                                                                                        |                                                                                                     aGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCt                                                                                                   >  1:126062/1‑139 (MQ=255)                tACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGtt                                                                                       <  1:477574/136‑1 (MQ=255)                tACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGtt                                                                                       >  2:477574/1‑136 (MQ=255)                    ggACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTa                                                                                <  1:212154/139‑1 (MQ=255)                                    aaCATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgcg                                                                >  1:257487/1‑139 (MQ=255)                                         caaaaaaCTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAg                                                           >  1:411728/2‑139 (MQ=255)                                          aaaaaaCTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTaacaac                                                                                            <  1:138757/105‑1 (MQ=255)                                          aaaaaaCTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTaacaac                                                                                            >  2:138757/1‑105 (MQ=255)                                                    cTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGa                                                <  2:550571/139‑1 (MQ=255)                                                          tGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACt                                          <  2:126062/139‑1 (MQ=255)                                                             tttCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACGCCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTgg                                       <  2:257487/139‑1 (MQ=255)                                                             tttCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTaacaac                                                                                            >  1:284116/1‑86 (MQ=255)                                                             tttCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTaacaac                                                                                            <  2:284116/86‑1 (MQ=255)                                                                ccGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCta                                    <  2:76325/139‑1 (MQ=255)                                                                   cTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCTGATAAACTTGGCTATaa                                 <  2:411728/139‑1 (MQ=255)                                                                                        tCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGc                                      <  1:58741/113‑1 (MQ=255)                                                                                        tCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGc                                      >  2:58741/1‑113 (MQ=255)                                                                                              cGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGaacaa      <  1:190590/139‑1 (MQ=255)                                                                                                  tGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCg  >  1:53879/1‑139 (MQ=255)                                                                                                                                        |                                                                                                     AGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCG  >  NC_000913/3936246‑3936481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑