Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,186,292	G→A	A315T (GCG→ACG)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,186,292	0	G	A	100.0%	135.6 / NA	40	A315T (GCG→ACG)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/20);  total (20/20)

GCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGT  >  NC_000913/4186161‑4186423                                                                                                                                    |                                                                                                                                    gcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                              >  2:38568/1‑139 (MQ=255) gcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                              <  2:210389/139‑1 (MQ=255) gcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                              >  1:418706/1‑139 (MQ=255)  cgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGGCGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAcc                                                                                                                             >  1:414568/1‑139 (MQ=255)  cgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAcc                                                                                                                             >  1:82908/1‑139 (MQ=255)  cgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                              >  2:95307/1‑138 (MQ=255)  cgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                              <  1:95307/138‑1 (MQ=255)        tAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCt                                                                                                                       <  2:414568/139‑1 (MQ=255)                 caacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGt                                                                                                              <  2:161521/139‑1 (MQ=255)                      gTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCctct                                                                                                         >  1:103177/1‑139 (MQ=255)                                      ggATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAGTCTTTGGCCGAc                                                                                         <  2:28799/139‑1 (MQ=255)                                         tCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATg                                                                                      >  1:610260/1‑139 (MQ=255)                                           tGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                    <  2:103177/139‑1 (MQ=255)                                             gCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCa                                                                                  >  1:43599/1‑139 (MQ=255)                                              cTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCaa                                                                                 >  1:561197/1‑139 (MQ=255)                                              cTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCaa                                                                                 >  2:181534/1‑139 (MQ=255)                                                                  cgtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTcc                                                             >  1:588603/1‑139 (MQ=255)                                                                       cGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAg                                                        >  2:498578/1‑139 (MQ=255)                                                                        gAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGa                                                       <  1:260832/139‑1 (MQ=255)                                                                         aaaaaCGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGaa                                                      <  2:610260/139‑1 (MQ=255)                                                                                    ctgcAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTa                                           >  2:72587/1‑139 (MQ=255)                                                                                       cAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGc                                        >  1:563859/1‑139 (MQ=255)                                                                                                    aCGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTAAAATCTTTCGCCGACATGATCAAAGGTAAACAGGGTCGTTTACGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTc                           >  1:166957/1‑139 (MQ=255)                                                                                                       cccTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCgg                        <  2:418706/139‑1 (MQ=255)                                                                                                         ctgctgGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTc                      <  2:563859/139‑1 (MQ=255)                                                                                                           gctgGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGt                    >  2:107840/1‑139 (MQ=255)                                                                                                           gctgGATAACGGTCGTCGCGGCCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGt                    <  1:200713/139‑1 (MQ=255)                                                                                                             tgGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTc                  <  2:43599/139‑1 (MQ=255)                                                                                                                 tAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTa              >  1:25121/1‑139 (MQ=255)                                                                                                                      gtcgtcGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTg                                                  <  2:560034/98‑1 (MQ=255)                                                                                                                      gtcgtcGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTg                                                  >  1:560034/1‑98 (MQ=255)                                                                                                                        cgtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCg       <  1:181534/139‑1 (MQ=255)                                                                                                                         gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                    >  2:500520/1‑61 (MQ=255)                                                                                                                         gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                    <  1:500520/61‑1 (MQ=255)                                                                                                                         gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCgg                        <  1:89822/121‑1 (MQ=255)                                                                                                                         gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCgg                        >  2:89822/1‑121 (MQ=255)                                                                                                                         gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt      <  2:561197/139‑1 (MQ=255)                                                                                                                         gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt      <  2:25121/139‑1 (MQ=255)                                                                                                                         gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt      <  2:588603/139‑1 (MQ=255)                                                                                                                             cgGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGt  <  2:166957/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    GCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGT  >  NC_000913/4186161‑4186423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑