Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,266,510 C→T A243A (GCC→GCT)  alr → alanine racemase, biosynthetic, PLP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,266,5100CT100.0% 104.9 / NA 31A243A (GCC→GCT) alralanine racemase, biosynthetic, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/13);  total (18/13)

TGGCCACAGTCGCATTTTGACTGGGTGCGCCCGGGCATCATTCTTTATGGCGTCTCGCCGCTGGAAGATCGCTCCACCGGTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCCGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCGAACGTGATACCCGTCTTGGCGTAGTCGCGATGGGCTATGGCGATGGTTATCCGCGCGCCGCGCCGTCCGGTACGCCA  >  NC_000913/4266379‑4266642
                                                                                                                                   |                                                                                                                                    
tgGCCACAGTCGCATTTTGACTGGGTGCGCCCGGGCATCATTCTTTATGGCGTCTCGCCGCTGGAAGATCGCTCCACCGGTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCtgtgcgtg                                                                                                                               >  1:535797/1‑139 (MQ=255)
  gCCACAGTCGCATTTTGACTGGGTGCGCCCGGGCATCATTCTTTATGGCGTCTCGCCGCTGGAAGATCGCTCCACCGGTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAg                                                                                                                             >  2:35625/1‑139 (MQ=255)
  gCCACAGTCGCATTTTGACTGGGTGCGCCCGGGCATCATTCTTTATGGCGTCTCGCCGCTGGAAGATCGCTCCACCGGTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAg                                                                                                                             >  2:242573/1‑139 (MQ=255)
    cacaGTCGCATTTTGACTGGGTGCGCCCGGGCATCATTCTTTATGGCGTCTCGCCGCTGGAAGATCGCTCCACCGGTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAGCa                                                                                                                           <  1:35625/139‑1 (MQ=255)
            cATTTTGACTGGGTTCGCCCGGGCATCATTCTTTATGGCGTCTCGCCGCTGGAAGATCGCTCCACCGGTGCCAATTTTGGCTGTCAGCCAGTGATGTCAGTAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCg                                                                                                                   >  2:400139/1‑139 (MQ=255)
            cATTTTGACTGGGTGCGCCCGGGCATCATTCTTTATGGCGTCTCGCCGCTGGAAGATCGCTCCACCGGTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCg                                                                                                                   >  1:647735/1‑139 (MQ=255)
                    cTGGGTGCGCCCGGGCATCATTCTTTATGGCGTCTCGCCGCTGGAAGATCGCTCCACCGGTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCt                                                                                                           <  1:370919/139‑1 (MQ=255)
                           cgcCCGGGCATCATTCTTTATGGCGTCTCGCCGCTGGAAGATCGCTCCACCGGTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGtt                                                                                                    <  1:361208/139‑1 (MQ=255)
                                 ggCATCATTCTTTATGGCGTCTCGCCGCTGGAAGATCGCTCCACCGGTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTAtggtg                                                                                              >  1:606569/1‑139 (MQ=255)
                                                ggCGTCTCGCCGCTGGAAGATCGCTCCACCGGTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCg                                                                               >  1:209680/1‑139 (MQ=255)
                                                   gTCTCGCCGCTGGAAGATCGCTCCACCGGTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCGAAc                                                                            >  1:630412/1‑139 (MQ=255)
                                                                    tCGCTCCACCGGTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCGAACGTGATACCCGTCTTGGc                                                           >  2:398914/1‑139 (MQ=255)
                                                                             cGGTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCGAACGTGATACCCGTCTTGGCGTAGTcgcg                                                  <  2:535797/139‑1 (MQ=255)
                                                                               gTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAGCa                                                                                                                           >  2:416068/1‑64 (MQ=255)
                                                                               gTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAGCa                                                                                                                           <  1:416068/64‑1 (MQ=255)
                                                                               gTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCGAACGTGATACCCGTCTTGGCGTAGTCGCGAt                                                >  1:185990/1‑139 (MQ=255)
                                                                                  ccGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCGAACGTGATAc                                                                     <  1:38553/115‑1 (MQ=255)
                                                                                  ccGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCGAACGTGATAc                                                                     >  2:38553/1‑115 (MQ=255)
                                                                                          ggCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCGAACGTGATACCCGTCTTGGCGTAGTCGCGATGGGCTATGGCg                                     <  1:400139/139‑1 (MQ=255)
                                                                                                      gTGATGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCGAACGTGATACCCGTCTTGGCGTAGTCGCGATGGGCTATGGCGATGGTTATCcgc                         <  2:185990/139‑1 (MQ=255)
                                                                                                         aTGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTAtggtg                                                                                              >  1:570221/1‑67 (MQ=255)
                                                                                                         aTGTCACTAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTAtggtg                                                                                              <  2:570221/67‑1 (MQ=255)
                                                                                                               cTAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCGAACGTGATACCCGTCTTGGCGTAGTCGCGATGGGCTATGGCGATGGTTATCCGcgcgccgcgc                <  2:262361/139‑1 (MQ=255)
                                                                                                                tAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCg                                                                               <  1:651005/75‑1 (MQ=255)
                                                                                                                tAACCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCg                                                                               >  2:651005/1‑75 (MQ=255)
                                                                                                                 aaCCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCGAACGTGATACCCGTCTTGGCGTAGTCGCGATGGGCTATGGCGATGGTTATCCGCGcgccgcgccg              >  1:320123/1‑139 (MQ=255)
                                                                                                                 aaCCTCCAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCGAACGTGATACCCGTCTTGGCGTAGTCGCGATGGGCTATGGCGATGGTTATCCGCGcgccgcgccg              >  1:161062/1‑139 (MQ=255)
                                                                                                                       cAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCGAACGTGATACCCGTCTTGGCGTAGTCGCGATGGGCt                                           <  2:319547/104‑1 (MQ=255)
                                                                                                                       cAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCGAACGTGATACCCGTCTTGGCGTAGTCGCGATGGGCt                                           >  1:319547/1‑104 (MQ=255)
                                                                                                                       cAGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCGAACGTGATACCCGTCTTGGCGTAGTCGCGATGGGCTATGGCGATGGTTATCCGCGCGCCGCGCCGTCCGGt        >  2:559185/1‑139 (MQ=255)
                                                                                                                        aGCCTGATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCGAACGTGATACCCGTCTTGGCGTAGTCGCGATGGGCTATGGCGATGGTTATCCGCGCGCCGCGCCGTCCGGTa       >  1:202416/1‑139 (MQ=255)
                                                                                                                             gATTGCTGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCGAACGTGATACCCGTCTTGGCGTAGTCGCGATGGGCTATGGCGATGGTTATCCGCGCGCCGCGCCGTCCGGTACGCCa  <  1:398914/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                    
TGGCCACAGTCGCATTTTGACTGGGTGCGCCCGGGCATCATTCTTTATGGCGTCTCGCCGCTGGAAGATCGCTCCACCGGTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGCCTGATTGCCGTGCGTGAGCATAAAGCCGGAGAGCCTGTTGGTTATGGTGGAACCTGGGTAAGCGAACGTGATACCCGTCTTGGCGTAGTCGCGATGGGCTATGGCGATGGTTATCCGCGCGCCGCGCCGTCCGGTACGCCA  >  NC_000913/4266379‑4266642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑