Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,361,357 A→G L193S (TTG→TCG)  cadC ← cadBA operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,361,3570AG100.0% 118.0 / NA 32L193S (TTG→TCG) cadCcadBA operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/18);  total (14/18)

GGAGAAGGTGTTAAGTGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGA  >  NC_000913/4361230‑4361490
                                                                                                                               |                                                                                                                                     
ggAGAAGGTGTTAAGTGCTGTCTCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGAt                                                                                                                            <  2:238167/139‑1 (MQ=255)
   gaAGGTGTTAAGTGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTg                                                                                                                         <  1:263801/139‑1 (MQ=255)
      ggTGTTAAGTGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTc                                                                                                                      <  1:160302/139‑1 (MQ=255)
               tGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGa                                                                                                             <  1:177364/139‑1 (MQ=255)
                 aTGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACg                                                                                                           >  2:417760/1‑139 (MQ=255)
                 aTGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACg                                                                                                           >  2:216455/1‑139 (MQ=255)
                       ccaccaAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATc                                                                                                     >  1:7731/1‑139 (MQ=255)
                                  cccACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGaaa                                                                                          <  2:2897/139‑1 (MQ=255)
                                         cTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTAc                                                                                   <  2:7731/139‑1 (MQ=255)
                                         cTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTAc                                                                                   <  1:258689/139‑1 (MQ=255)
                                                 tAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTa                                                                           <  1:417760/139‑1 (MQ=255)
                                                   agagagCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTaca                                                                         <  2:449804/139‑1 (MQ=255)
                                                         cTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATa                                                                   >  1:323648/1‑139 (MQ=255)
                                                            aTACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGAc                                                                                                            >  1:29371/1‑95 (MQ=255)
                                                            aTACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGAc                                                                                                            <  2:29371/95‑1 (MQ=255)
                                                                      cTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGacaaca                                                      >  1:12463/1‑139 (MQ=255)
                                                                              gTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGaaaaaa                                              >  2:474217/1‑139 (MQ=255)
                                                                                   aaCTTTGATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCa                                         <  1:316290/139‑1 (MQ=255)
                                                                                         tatttaCCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTAc                                                                                   >  1:601111/1‑91 (MQ=255)
                                                                                         tatttaCCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTAc                                                                                   <  2:601111/91‑1 (MQ=255)
                                                                                                            aaTATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAAc                                                                                        >  2:278075/1‑67 (MQ=255)
                                                                                                            aaTATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAAc                                                                                        <  1:278075/67‑1 (MQ=255)
                                                                                                             atatCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTaca                                                                         <  2:543942/81‑1 (MQ=255)
                                                                                                             atatCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTaca                                                                         >  1:543942/1‑81 (MQ=255)
                                                                                                             atatCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGc               >  1:235821/1‑139 (MQ=255)
                                                                                                              tatCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTAc                                                                                   <  2:406716/70‑1 (MQ=255)
                                                                                                              tatCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGacaaca                                                      <  2:309253/99‑1 (MQ=255)
                                                                                                              tatCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGacaaca                                                      >  1:309253/1‑99 (MQ=255)
                                                                                                              tatCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATAAAGACTTGAAAACGCTAc                                                                                   >  1:406716/1‑70 (MQ=255)
                                                                                                               atCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCtt             <  2:188933/139‑1 (MQ=255)
                                                                                                                   cgTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTaa         >  2:59448/1‑139 (MQ=255)
                                                                                                                          tGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGa  <  2:323648/139‑1 (MQ=255)
                                                                                                                               |                                                                                                                                     
GGAGAAGGTGTTAAGTGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGA  >  NC_000913/4361230‑4361490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: