| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,575,425 | A→G | pseudogene (1507/2937 nt) | yjiV → | pseudogene, conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,575,425 | 0 | A | G | 96.3% | 88.9 / ‑6.0 | 27 | pseudogene (1507/2937 nt) | yjiV | pseudogene, conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (10/16); total (10/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
GATCATTACGTTCGCGAGAGCAACTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACAGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGC > NC_000913/4575299‑4575542 | gATCATTACGTTCGCGAGAGCAACTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTc > 1:413543/1‑139 (MQ=255) cgcgAGAGCAACTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATg > 2:192173/1‑139 (MQ=255) cAACTTCACACAGCTGGATGATGAGCTGAAAAACTGTATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTa < 2:413543/139‑1 (MQ=255) cTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATcc > 1:537659/1‑139 (MQ=255) cTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAg > 2:441354/1‑139 (MQ=255) cTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAg > 2:521528/1‑139 (MQ=255) gatgatGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGa > 1:10366/1‑139 (MQ=255) aGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCt > 1:199454/1‑139 (MQ=255) aGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCt > 1:80021/1‑139 (MQ=255) tGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTggggg < 1:441354/139‑1 (MQ=255) gaatGGTAGCCGTTATTAATAAAAATTCGTCCGTAACCCGGAGTCAAAAGATCCTGACGATAATCGGAACGGACGCTGGTCTGAAAGTCGTAATGGCAACGTATCCGATCGTTTAGCGAAGTTGCTGATGCTGGGGGCt < 1:334806/136‑1 (MQ=255) gATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCt < 1:192173/139‑1 (MQ=255) ttGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCaa > 1:381439/1‑97 (MQ=255) ttGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCaa < 2:381439/97‑1 (MQ=255) aaTTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGc < 2:434282/139‑1 (MQ=255) aaTTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGc < 2:655998/139‑1 (MQ=255) cGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTg < 1:287738/139‑1 (MQ=255) tAACCCGGAATCAAAATATCCGGAAGATAAGCAGAACAGACGCTGGCCTCAAATTCGTAATGGCTACGTATCCCAACGTTTAGCGAAGTTGCGAATGCTGGGGGCTGGATTCAAAACCGTCAATGCCGCAACTATTGat < 1:551474/139‑1 (MQ=255) tAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGat < 2:199454/139‑1 (MQ=255) tAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGat < 2:224068/139‑1 (MQ=255) tAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGat < 1:521528/139‑1 (MQ=255) cGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGAtattat < 2:80021/139‑1 (MQ=255) cGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGAtattat < 2:388950/139‑1 (MQ=255) cGGAATCAAAAGATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGAtattat < 2:321480/139‑1 (MQ=255) gATCCTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGc < 2:10366/139‑1 (MQ=255) cTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCa > 2:499465/1‑55 (MQ=255) cTGAAGATAATCAGAACGGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCa < 1:499465/55‑1 (MQ=255) | GATCATTACGTTCGCGAGAGCAACTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACAGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGC > NC_000913/4575299‑4575542 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |