Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	663,640	A→G	G108G (GGT→GGC)	dacA ←	D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	663,640	0	A	G	100.0%	56.9 / NA	17	G108G (GGT→GGC)	dacA	D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/10);  total (7/10)

CAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCCGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGCTGGCAGGATCGCGGCGGACATCT  >  NC_000913/663507‑663769                                                                                                                                      |                                                                                                                                  cAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCCGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTGCCGGt                                                                                                                              <  2:506486/139‑1 (MQ=255)      gCAAAATCGGCCATGGCGACACAAGCATCGTTACCCGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTGCCGGTGGccc                                                                                                                         >  2:164583/1‑139 (MQ=255)             cGGCCATGGCGACACAAGCATCGTTACCCGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTGCCGGTGGCCCATGcgtc                                                                                                                  <  2:541812/139‑1 (MQ=255)                         cacaAGCATCGTTACCCGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTGCCGGTGGCCCATGCGTCGTTGCCGATAGt                                                                                                      <  1:120805/139‑1 (MQ=255)                                   gTTACCCGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTGCCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCa                                                                                            >  2:12680/1‑139 (MQ=255)                                            ttGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTGCCGGTGGCCCATGcgtc                                                                                                                  >  2:109213/1‑108 (MQ=255)                                            ttGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTGCCGGTGGCCCATGcgtc                                                                                                                  <  1:109213/108‑1 (MQ=255)                                                      aTACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTGCCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCgg                                                                         >  2:318382/1‑139 (MQ=255)                                                            cgGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTGCCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCa                                                                   >  1:66990/1‑139 (MQ=255)                                                                 cAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTGCCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATCTACCGGCTTTCATTGcc                                                              <  1:376360/139‑1 (MQ=255)                                                                         aaaCCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTGCCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGAt                                                      >  2:586207/1‑139 (MQ=255)                                                                                   cTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTGCCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTg                                            >  2:418112/1‑139 (MQ=255)                                                                                          cccGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTGCCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGtcatca                                     <  1:164583/139‑1 (MQ=255)                                                                                            cGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTGCCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCAtt                                   <  1:586207/139‑1 (MQ=255)                                                                                                  gAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTGCCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTc                             <  2:66990/139‑1 (MQ=255)                                                                                                                 agaaCCTTTAAACACCGGGTTGCCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGCTGGCAGGATcg              <  1:418112/139‑1 (MQ=255)                                                                                                                             cacCGGGTTGCCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGCTGGCAGGATCGCGGCGGACATCt  <  2:647808/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  CAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCCGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGCTGGCAGGATCGCGGCGGACATCT  >  NC_000913/663507‑663769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑