Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,190,061 (G)6→7 coding (387/672 nt) phoP ← response regulator in two‑component regulatory system with PhoQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,190,0551.G97.9% 361.6 / ‑0.7 96coding (393/672 nt)phoPresponse regulator in two‑component regulatory system with PhoQ
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base G (51/43);  total (52/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

TTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAA  >  NC_000913/1189917‑1190177
                                                                                                                                           |                                                                                                                           
ttGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAAc                                                                                                                              >  2:1089413/1‑139 (MQ=255)
 tGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACg                                                                                                                            <  1:1371016/139‑1 (MQ=255)
                   ttGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGAcc                                                                                                          <  2:1291283/139‑1 (MQ=255)
                             cTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGTCa                                                                                                <  2:349084/139‑1 (MQ=255)
                              tttGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAg                                                                                               >  1:514837/1‑139 (MQ=255)
                                tGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAAt                                                                                                              <  2:542776/122‑1 (MQ=255)
                                tGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAAt                                                                                                              >  1:542776/1‑122 (MQ=255)
                                     ttattGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTa                                                                                        >  1:1371529/1‑139 (MQ=255)
                                     ttattGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTa                                                                                        >  2:400976/1‑139 (MQ=255)
                                             tATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTAcgccg                                                                                >  1:168477/1‑139 (MQ=255)
                                             tATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTAcgccg                                                                                >  2:429730/1‑139 (MQ=255)
                                             tATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTAcg                                                                                   <  2:598583/136‑1 (MQ=255)
                                             tATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTAcg                                                                                   >  1:598583/1‑136 (MQ=255)
                                                       tCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGcc                                                                      >  2:283480/1‑139 (MQ=255)
                                                        ccATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCt                                                                     >  2:650525/1‑139 (MQ=255)
                                                             ataGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCAtt                                                                >  2:848878/1‑139 (MQ=255)
                                                             ataGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCAtt                                                                >  2:299964/1‑139 (MQ=255)
                                                             ataGTGTATTCGAACGCGGTCAGATTGATCACTTCGTCATTAATAGATAATTCACAGCGAGAGAGAGCAACCTGAAAAGGGGGAGAGCGAAATAACCAGTGAAACGAAAAAGGTATTAGGCCACATTAATGATTGCaat                                                                >  2:26524/1‑137 (MQ=255)
                                                                gtgtATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTcgc                                                             >  1:671641/1‑139 (MQ=255)
                                                                 tgtATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGACACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTcgcg                                                            >  2:1480658/1‑139 (MQ=255)
                                                                 tgtATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGaa                                                                                                                <  1:338426/87‑1 (MQ=255)
                                                                 tgtATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGaa                                                                                                                >  2:338426/1‑87 (MQ=255)
                                                                 tgtATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTcgcg                                                            >  1:472069/1‑139 (MQ=255)
                                                                 tgtATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTcgcg                                                            >  2:1230719/1‑139 (MQ=255)
                                                                  gtATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCgcgc                                                           >  2:1223202/1‑139 (MQ=255)
                                                                   tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGa                                                                                                            <  1:612199/89‑1 (MQ=255)
                                                                   tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGa                                                                                                            <  2:1258442/89‑1 (MQ=255)
                                                                   tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGa                                                                                                            >  2:612199/1‑89 (MQ=255)
                                                                   tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGa                                                                                                            >  1:1258442/1‑89 (MQ=255)
                                                                   tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCa                                                                                                <  2:423015/101‑1 (MQ=255)
                                                                   tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCa                                                                                                >  1:423015/1‑101 (MQ=255)
                                                                   tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGcc                                                          >  1:258989/1‑139 (MQ=255)
                                                                   tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGcc                                                          >  1:1238882/1‑139 (MQ=255)
                                                                   tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGcc                                                          >  2:1334444/1‑139 (MQ=255)
                                                                   tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGcc                                                          <  2:168477/139‑1 (MQ=255)
                                                                   tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGcc                                                          >  1:1412585/1‑139 (MQ=255)
                                                                   tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGcc                                                          <  1:1416885/139‑1 (MQ=255)
                                                                    aTTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAg                                                                                               <  1:359917/101‑1 (MQ=255)
                                                                    aTTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAg                                                                                               >  2:359917/1‑101 (MQ=255)
                                                                     ttCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCAt                                                        <  1:429730/139‑1 (MQ=255)
                                                                     ttCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCAt                                                        >  2:1146859/1‑139 (MQ=255)
                                                                      tCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCa                                                                                                <  1:946681/98‑1 (MQ=255)
                                                                      tCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCa                                                                                                >  2:946681/1‑98 (MQ=255)
                                                                        gAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCAc                                                     <  2:472069/139‑1 (MQ=255)
                                                                        gAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCAc                                                     <  2:597900/139‑1 (MQ=255)
                                                                           cgcgGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACggggggg                                                                                                                     >  2:962517/1‑70 (MQ=255)
                                                                           cgcgGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACggggggg                                                                                                                     <  1:962517/71‑2 (MQ=255)
                                                                               gTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCAc                                                     >  1:855237/1‑132 (MQ=255)
                                                                               gTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCAc                                                     <  2:855237/132‑1 (MQ=255)
                                                                                    tttGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATg                                         >  1:803934/1‑139 (MQ=255)
                                                                                     ttGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGa                                        <  1:26524/139‑1 (MQ=255)
                                                                                     ttGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGa                                        <  1:299964/139‑1 (MQ=255)
                                                                                      tGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGaa                                       <  1:1146859/139‑1 (MQ=255)
                                                                                          cACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACgg                                   >  2:542449/1‑139 (MQ=255)
                                                                                          cACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACgg                                   >  2:807923/1‑139 (MQ=255)
                                                                                            cTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGtt                                 <  2:514837/139‑1 (MQ=255)
                                                                                            cTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGtt                                 <  1:1223202/139‑1 (MQ=255)
                                                                                             ttCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGttt                                >  1:244572/1‑139 (MQ=255)
                                                                                              tCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTa                               <  1:848878/139‑1 (MQ=255)
                                                                                              tCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTa                               <  2:1238882/139‑1 (MQ=255)
                                                                                              tCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTa                               >  1:770253/1‑139 (MQ=255)
                                                                                              tCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGAc                                                                                             >  1:378890/1‑76 (MQ=255)
                                                                                              tCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGAc                                                                                             <  2:378890/76‑1 (MQ=255)
                                                                                                   aTTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTcac                          >  1:522822/1‑139 (MQ=255)
                                                                                                    ttAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTcaca                         <  1:1345346/139‑1 (MQ=255)
                                                                                                    ttAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTcaca                         <  1:542449/139‑1 (MQ=255)
                                                                                                    ttAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTcaca                         <  2:244572/139‑1 (MQ=255)
                                                                                                       aTAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATg                                                                                                             <  1:1125851/52‑1 (MQ=255)
                                                                                                       aTAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATg                                                                                                             >  2:1125851/1‑52 (MQ=255)
                                                                                                       aTAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCAt                                                        <  2:2369/105‑1 (MQ=255)
                                                                                                       aTAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCAt                                                        >  1:2369/1‑105 (MQ=255)
                                                                                                       aTAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATaa                      >  1:949852/1‑139 (MQ=255)
                                                                                                         aGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAatc                    <  2:949852/139‑1 (MQ=255)
                                                                                                              aTTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTa                                                                                        <  1:132766/66‑1 (MQ=255)
                                                                                                              aTTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTa                                                                                        >  2:132766/1‑66 (MQ=255)
                                                                                                              aTTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAg               <  2:803934/139‑1 (MQ=255)
                                                                                                               ttCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGa                                                                                              >  2:1040576/1‑59 (MQ=255)
                                                                                                               ttCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGa                                                                                              <  1:1040576/59‑1 (MQ=255)
                                                                                                               ttCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGc              >  1:409271/1‑139 (MQ=255)
                                                                                                               ttCACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGc              >  1:1152401/1‑139 (MQ=255)
                                                                                                                 cACGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCAc            <  1:1089413/139‑1 (MQ=255)
                                                                                                                   cGGCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCg          <  1:748551/139‑1 (MQ=255)
                                                                                                                    ggCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCAc                                                     <  2:1399985/95‑1 (MQ=255)
                                                                                                                    ggCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCAc                                                     >  1:1399985/1‑95 (MQ=255)
                                                                                                                    ggCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCtt                                                >  1:655709/1‑100 (MQ=255)
                                                                                                                    ggCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCtt                                                <  2:655709/100‑1 (MQ=255)
                                                                                                                    ggCGAGAGAGATCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCgg         >  1:57848/1‑139 (MQ=255)
                                                                                                                         gagagaTCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCa                                                                                                <  1:990518/47‑1 (MQ=255)
                                                                                                                         gagagaTCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATc                                                       >  1:314198/1‑88 (MQ=255)
                                                                                                                         gagagaTCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATc                                                       <  2:314198/88‑1 (MQ=255)
                                                                                                                         gagagaTCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACtt    >  1:583774/1‑139 (MQ=255)
                                                                                                                           gagaTCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTaa  <  2:258989/139‑1 (MQ=255)
                                                                                                                              aTCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGAc                                                                                             >  1:355176/1‑45 (MQ=255)
                                                                                                                              aTCAACCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGAc                                                                                             <  2:355176/45‑1 (MQ=255)
                                                                                                                                  aCCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCa                                                                                                >  1:488459/1‑38 (MQ=255)
                                                                                                                                  aCCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCa                                                                                                <  2:488459/38‑1 (MQ=255)
                                                                                                                                  aCCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCAc            >  2:1504652/1‑122 (MQ=255)
                                                                                                                                  aCCTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCAc            <  1:1504652/122‑1 (MQ=255)
                                                                                                                                   ccTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCAc                                                     <  2:330220/80‑1 (MQ=255)
                                                                                                                                   ccTGAAACGGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCAc                                                     >  1:330220/1‑80 (MQ=255)
                                                                                                                                           |                                                                                                                           
TTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAA  >  NC_000913/1189917‑1190177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: