Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,212,088 (C)8→9 intergenic (‑85/+615) iraM ← / ← ycgX RpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,212,0801.C100.0% 61.6 / NA 18intergenic (‑77/+623)iraM/ycgXRpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (12/6);  total (12/6)

AAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATT  >  NC_000913/1211949‑1212213
                                                                                                                                    |                                                                                                                                     
aaaaaGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAccccccc                                                                                                                                >  2:719016/1‑139 (MQ=255)
                         aaTTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAGAAATAAATTAATTaaaa                                                                                                        >  1:582952/1‑139 (MQ=255)
                           ttACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                                      <  2:52030/139‑1 (MQ=255)
                                gtgtCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGc                                                                                                 <  1:719016/139‑1 (MQ=255)
                                   tCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTta                                                                                              >  2:969907/1‑139 (MQ=255)
                                                    cATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTaa                                                                             >  1:831844/1‑139 (MQ=255)
                                                    cATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAccccccccctcaa                                                                                                                          >  1:452290/1‑88 (MQ=255)
                                                    cATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAccccccccctcaa                                                                                                                          <  2:452290/93‑6 (MQ=255)
                                                     aTTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTaaa                                                                            >  2:850752/1‑139 (MQ=255)
                                                                    tCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                                      <  2:1003029/98‑1 (MQ=255)
                                                                    tCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                                      >  1:1003029/1‑98 (MQ=255)
                                                                    tCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAAt                                                             <  1:719964/139‑1 (MQ=255)
                                                                       cACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGa                                                          >  2:586448/1‑139 (MQ=255)
                                                                                        ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATgaga                                         >  2:1462818/1‑139 (MQ=255)
                                                                                        ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTAGATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAAAAAAATgaga                                         >  1:996653/1‑139 (MQ=255)
                                                                                          cATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAtt                                       >  2:911552/1‑139 (MQ=255)
                                                                                            ttAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGAAAGGAATCTCAATGGATGTTAAACAAAATGAGAtttt                                     >  2:949611/1‑139 (MQ=255)
                                                                                                                  ttGCATATGAGTATATTACCCCCCCCCTCAAAGAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGAc               <  2:582952/139‑1 (MQ=255)
                                                                                                                               tatTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGAtt  >  2:1450092/1‑139 (MQ=255)
                                                                                                                               tatTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGAt   >  2:1117589/1‑138 (MQ=255)
                                                                                                                               tatTACCCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGAt   <  1:1117589/138‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                     
AAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATT  >  NC_000913/1211949‑1212213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: