Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,351,196	(G)7→8	intergenic (‑157/+211)	fabI ← / ← ycjD	enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,351,189	1	.	G	100.0%	217.7 / NA	59	intergenic (‑150/+218)	fabI/ycjD	enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (31/28);  total (31/28)

TATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGA  >  NC_000913/1351052‑1351319                                                                                                                                           |                                                                                                                                    tATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGTCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑g                                                                                                                                   >  2:418829/1‑139 (MQ=255)        gATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑gggggggg                                                                                                                            >  2:10728/1‑138 (MQ=255)                    gCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCt                                                                                                                 >  1:877391/1‑139 (MQ=255)                     ccTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTc                                                                                                                >  2:1252040/1‑139 (MQ=255)                         aaaaTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCAccc                                                                                                            >  2:369820/1‑139 (MQ=255)                             tCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAAc                                                                                                        <  1:10728/139‑1 (MQ=255)                              cAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATTAACGGTTATTATAATCAACCTGGCTGGGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAAcc                                                                                                       <  1:316584/139‑1 (MQ=255)                                ggCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCct                                                                                                     <  1:1350956/139‑1 (MQ=255)                                     ttCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTccc                                                                                                >  1:1167700/1‑139 (MQ=255)                                                aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                     <  1:1252040/139‑1 (MQ=255)                                                aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                     <  2:362619/139‑1 (MQ=255)                                                aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                     <  2:429021/139‑1 (MQ=255)                                                aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                     <  1:369820/139‑1 (MQ=255)                                                     aCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACGGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGATGGGa                                                                                <  2:877391/139‑1 (MQ=255)                                                         tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGCGGGGCGAGGGGACCGt                                                                            >  1:602716/1‑139 (MQ=255)                                                         tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGt                                                                            >  2:557470/1‑139 (MQ=255)                                                         tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGt                                                                            >  2:791923/1‑139 (MQ=255)                                                          aCGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                           <  1:557470/139‑1 (MQ=255)                                                          aCGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGAGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                           <  1:418829/139‑1 (MQ=255)                                                           cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAt                                                                          >  2:1300022/1‑139 (MQ=255)                                                           cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAt                                                                          <  2:602716/139‑1 (MQ=255)                                                              aCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                     <  1:1455405/125‑1 (MQ=255)                                                              aCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                     >  2:1455405/1‑125 (MQ=255)                                                                 gATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa                                                                    >  1:1390605/1‑139 (MQ=255)                                                                   tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaaa                                                                  >  1:374776/1‑139 (MQ=255)                                                                            attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCttt                                                                                                                       >  1:262610/1‑77 (MQ=255)                                                                            attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCttt                                                                                                                       <  2:262610/77‑1 (MQ=255)                                                                            attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAc                                                         >  1:1290512/1‑139 (MQ=255)                                                                            attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAc                                                         >  2:66183/1‑139 (MQ=255)                                                                                   tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTccc                                                                                                                   <  1:850489/74‑1 (MQ=255)                                                                                   tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTccc                                                                                                                   >  2:850489/1‑74 (MQ=255)                                                                                       ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCt                                                                                                                 >  1:448921/1‑72 (MQ=255)                                                                                       ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCt                                                                                                                 <  2:448921/72‑1 (MQ=255)                                                                                       ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTACCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGcaacaa                                              >  1:998487/1‑139 (MQ=255)                                                                                        cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             >  1:949956/1‑139 (MQ=255)                                                                                        cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             <  2:1290512/139‑1 (MQ=255)                                                                                        cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             <  1:791923/139‑1 (MQ=255)                                                                                          ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa                                                                    <  1:907270/114‑1 (MQ=255)                                                                                          ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa                                                                    >  2:907270/1‑114 (MQ=255)                                                                                            cTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCCCCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAgg                                         >  2:990461/1‑139 (MQ=255)                                                                                                 aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCAt                                    >  1:1340072/1‑139 (MQ=255)                                                                                                     gCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa                                                                    >  1:466955/1‑103 (MQ=255)                                                                                                     gCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa                                                                    <  2:466955/103‑1 (MQ=255)                                                                                                         taGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGcccc                            >  1:882435/1‑139 (MQ=255)                                                                                                              gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAc                        >  2:497205/1‑139 (MQ=255)                                                                                                              gCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                     <  1:284104/77‑1 (MQ=255)                                                                                                              gCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                     >  2:284104/1‑77 (MQ=255)                                                                                                               ccAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCt                                                                                                                 <  1:634344/48‑1 (MQ=255)                                                                                                               ccAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCt                                                                                                                 >  2:634344/1‑48 (MQ=255)                                                                                                               ccAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             <  2:1253136/116‑1 (MQ=255)                                                                                                               ccAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             >  1:1253136/1‑116 (MQ=255)                                                                                                                cAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                     >  1:230913/1‑75 (MQ=255)                                                                                                                cAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                     <  2:230913/75‑1 (MQ=255)                                                                                                                cAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgtg                                                                      >  1:556192/1‑90 (MQ=255)                                                                                                                cAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgtg                                                                      <  2:556192/90‑1 (MQ=255)                                                                                                                        aCCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGc             >  1:696310/1‑139 (MQ=255)                                                                                                                             aGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCag                                                                                             <  1:838150/54‑1 (MQ=21)                                                                                                                             aGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCag                                                                                             >  2:838150/1‑54 (MQ=21)                                                                                                                                   cTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  2:696310/139‑1 (MQ=255)                                                                                                                                   cTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  1:1300022/139‑1 (MQ=255)                                                                                                                                   cTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  2:1140964/139‑1 (MQ=255)                                                                                                                                           |                                                                                                                                    TATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGA  >  NC_000913/1351052‑1351319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑