Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 304,952 (G)6→7 coding (254/711 nt) ecpE ← ECP production pilus chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913304,9461.G100.0% 248.4 / NA 65R87P (CGC→CCC) ecpEECP production pilus chaperone
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (32/33);  total (32/33)

GCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAA  >  NC_000913/304819‑305078
                                                                                                                                |                                                                                                                                    
gCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCgggggggcgaa                                                                                                                           >  2:1280421/1‑134 (MQ=255)
       gTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGt                                                                                                                     <  1:982999/139‑1 (MQ=255)
        tATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCTAGCTGGCGGGGGGGCGAAAAGCAGtt                                                                                                                    <  1:871085/139‑1 (MQ=255)
        tATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGtt                                                                                                                    <  1:645466/139‑1 (MQ=255)
              tACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCAt                                                                                                              >  1:1191463/1‑139 (MQ=255)
               aCGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATc                                                                                                             >  2:1183123/1‑139 (MQ=255)
                 gAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCa                                                                                                           <  1:593167/139‑1 (MQ=255)
                  aGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCAc                                                                                                          >  2:343856/1‑139 (MQ=255)
                     ggggACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCgg                                                                                                       >  2:372945/1‑139 (MQ=255)
                      gggACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGt                                                                                                      >  2:91965/1‑139 (MQ=255)
                      gggACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGt                                                                                                      >  1:527074/1‑139 (MQ=255)
                      gggACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGt                                                                                                      >  2:4496/1‑139 (MQ=255)
                      gggACCTCGCGAAATGAGACCCGGAAGTAGCGCTCGCGGTTATCCCGTGGACCATGAAAGAAAAATTTAAAATACTCCCTCTCACCAGCCTGCACCGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACAACCCACCGGt                                                                                                      >  2:1474317/1‑139 (MQ=255)
                       ggACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTc                                                                                                     >  2:167165/1‑139 (MQ=255)
                         aCCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCgg                                                                                                   <  1:91965/139‑1 (MQ=255)
                                gAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCa                                                                                            <  1:1474317/139‑1 (MQ=255)
                                             gtagtaGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGcc                                                                               <  1:1280421/139‑1 (MQ=255)
                                              tagtagCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGccc                                                                              <  1:695327/139‑1 (MQ=255)
                                                 tagCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCggg                                                                           <  1:343856/139‑1 (MQ=255)
                                                 tagCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCggg                                                                           <  1:1037154/139‑1 (MQ=255)
                                                  agCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGc                                                                          <  2:1191463/139‑1 (MQ=255)
                                                      cTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATc                                                                      >  1:211226/1‑139 (MQ=255)
                                                      cTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATc                                                                      >  1:192797/1‑139 (MQ=255)
                                                      cTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATc                                                                      >  2:1254752/1‑139 (MQ=255)
                                                        cgcgGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTAt                                                                       <  2:550245/136‑1 (MQ=255)
                                                        cgcgGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTAt                                                                       >  1:550245/1‑136 (MQ=255)
                                                        cgcgGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCaa                                                                    >  2:936986/1‑139 (MQ=255)
                                                               aTCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGt                                                                                                 >  2:1065494/1‑103 (MQ=255)
                                                               aTCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGt                                                                                                 <  1:1065494/103‑1 (MQ=255)
                                                                  ccGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGAt                                                          <  2:211226/139‑1 (MQ=255)
                                                                     tGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGc                                                       >  1:985567/1‑139 (MQ=255)
                                                                       gACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCaa                                                     >  1:1217319/1‑139 (MQ=255)
                                                                       gACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCaa                                                     <  2:1217319/139‑1 (MQ=255)
                                                                       gACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCaa                                                     <  1:936986/139‑1 (MQ=255)
                                                                         ccATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATa                                                   <  1:1467347/139‑1 (MQ=255)
                                                                            tGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCt                                                                         <  1:1477181/114‑1 (MQ=255)
                                                                            tGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCt                                                                         >  2:1477181/1‑114 (MQ=255)
                                                                            tGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACgg                                                >  1:1076073/1‑139 (MQ=255)
                                                                             gATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCTGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCaa                                                     <  1:1176393/133‑1 (MQ=255)
                                                                             gATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCTGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCaa                                                     >  2:1176393/1‑133 (MQ=255)
                                                                                       tttAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCaa                                                     >  1:816196/1‑123 (MQ=255)
                                                                                       tttAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCaa                                                     <  2:816196/123‑1 (MQ=255)
                                                                                       tttAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTgc                                     >  1:29414/1‑139 (MQ=255)
                                                                                            aaTACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCttt                                <  1:372945/139‑1 (MQ=255)
                                                                                                 tCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCaa                                                     <  1:632492/113‑1 (MQ=255)
                                                                                                 tCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCaa                                                     >  2:632492/1‑113 (MQ=255)
                                                                                                 tCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTttgttg                           <  1:167165/139‑1 (MQ=255)
                                                                                                   gCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTgttgtt                         <  1:1183123/139‑1 (MQ=255)
                                                                                                    ctctCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTa                        <  1:127073/139‑1 (MQ=255)
                                                                                                    ctctCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTa                        <  2:192797/139‑1 (MQ=255)
                                                                                                    ctctCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTa                        <  1:1254752/139‑1 (MQ=255)
                                                                                                    ctctCACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTa                        <  1:4496/139‑1 (MQ=255)
                                                                                                      ctcACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCt                                                                         >  1:1067457/1‑88 (MQ=255)
                                                                                                      ctcACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGCTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCt                                                                         <  2:1067457/88‑1 (MQ=255)
                                                                                                        cACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCAc                                                                                                                 <  2:1374570/46‑1 (MQ=255)
                                                                                                        cACCAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCAc                                                                                                                 >  1:1374570/1‑46 (MQ=255)
                                                                                                          ccAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGc                                                                >  2:132842/1‑93 (MQ=255)
                                                                                                          ccAGCCTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGc                                                                <  1:132842/93‑1 (MQ=255)
                                                                                                              ccTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCa                                                                                                                  >  2:947781/1‑39 (MQ=255)
                                                                                                              ccTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCa                                                                                                                  <  1:947781/39‑1 (MQ=255)
                                                                                                              ccTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTacac              >  2:163346/1‑139 (MQ=255)
                                                                                                              ccTGCAACGCCAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTacac              >  1:950975/1‑139 (MQ=255)
                                                                                                                   aaCGCCATCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTg         >  2:1284106/1‑139 (MQ=255)
                                                                                                                        cAGCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCCCCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGAc    <  2:510657/139‑1 (MQ=255)
                                                                                                                          gCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACaa  >  1:1326183/1‑139 (MQ=255)
                                                                                                                          gCTGGCGGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACaa  >  1:1453040/1‑139 (MQ=255)
                                                                                                                                |                                                                                                                                    
GCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAA  >  NC_000913/304819‑305078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: