Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	924,093	A→G	D277G (GAC→GGC)	clpA →	ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	924,093	0	A	G	100.0%	166.1 / NA	45	D277G (GAC→GGC)	clpA	ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/23);  total (22/23)

CAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATT  >  NC_000913/923960‑924220                                                                                                                                      |                                                                                                                                cAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTa                                                                                                                            >  2:1042402/1‑139 (MQ=255)      cGATGTGCCGGAAGTGATGGCTGTCTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCTGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGc                                                                                                                       <  1:1308603/139‑1 (MQ=255)        aTGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCAt                                                                                                                     <  2:120392/139‑1 (MQ=255)         tgtgCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATc                                                                                                                    <  2:201044/139‑1 (MQ=255)           tgCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCt                                                                                                                  <  1:1006020/139‑1 (MQ=255)                     gATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATtgat                                                                                                        <  1:1042402/139‑1 (MQ=255)                      aTGGCTGACTGTACGATTTACTCTCTCAATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATtgatg                                                                                                       <  2:819665/139‑1 (MQ=255)                       tGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATtgatga                                                                                                      >  1:1094652/1‑139 (MQ=255)                              cTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCcac                                                                                               <  2:658864/139‑1 (MQ=255)                                         aCTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATc                                                                                                                    >  1:102562/1‑107 (MQ=255)                                         aCTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATc                                                                                                                    <  2:102562/107‑1 (MQ=255)                                         aCTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCgg                                                                                    <  1:404680/139‑1 (MQ=255)                                           tctctcGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATc                                                                                      >  1:223486/1‑135 (MQ=255)                                           tctctcGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATc                                                                                      <  2:223486/135‑1 (MQ=255)                                              ctcGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCgg                                                                               >  1:363907/1‑139 (MQ=255)                                                         ttCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACa                                                                                                                         <  2:77268/86‑1 (MQ=255)                                                         ttCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACa                                                                                                                         >  1:77268/1‑86 (MQ=255)                                                         ttCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCt                                                                    >  1:1328390/1‑139 (MQ=255)                                                          tctcTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCtg                                                                   <  2:1094652/139‑1 (MQ=255)                                                           ctctGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGc                                                                                                                       <  2:3833/86‑1 (MQ=255)                                                           ctctGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGc                                                                                                                       >  1:3833/1‑86 (MQ=255)                                                            tctGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCa                                                                          <  1:341701/130‑1 (MQ=255)                                                            tctGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCa                                                                          >  2:341701/1‑130 (MQ=255)                                                              tGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCtggtgg                                                               >  1:724937/1‑139 (MQ=255)                                                                   gCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATc                                                                                                                    <  1:1036141/81‑1 (MQ=255)                                                                   gCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATc                                                                                                                    >  2:1036141/1‑81 (MQ=255)                                                                       gCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGa                                                      <  2:363907/139‑1 (MQ=255)                                                                        cacaAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATgag                                                                                                     >  2:705312/1‑91 (MQ=255)                                                                        cacaAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATgag                                                                                                     <  1:705312/91‑1 (MQ=255)                                                                                     ggcgACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTaa                                        >  2:52045/1‑139 (MQ=255)                                                                                      gcgACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATc                                                                                                                    >  2:238692/1‑62 (MQ=255)                                                                                      gcgACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATc                                                                                                                    <  1:238692/62‑1 (MQ=255)                                                                                             tGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCg                                >  2:1173787/1‑139 (MQ=255)                                                                                                      ttttAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTcc                       <  1:1173787/139‑1 (MQ=255)                                                                                                              cGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCcac                                                                                               <  1:222181/59‑1 (MQ=255)                                                                                                              cGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCcac                                                                                               >  2:222181/1‑59 (MQ=255)                                                                                                               gTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATcc                                                                                                                   >  2:1122685/1‑38 (MQ=255)                                                                                                               gTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATcc                                                                                                                   <  1:1122685/38‑1 (MQ=255)                                                                                                               gTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTaaa              >  1:354594/1‑139 (MQ=255)                                                                                                                ttGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTaaaa             >  1:480909/1‑139 (MQ=255)                                                                                                                 tGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTAt                                                                                                            <  2:1357672/43‑1 (MQ=255)                                                                                                                 tGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTAt                                                                                                            >  1:1357672/1‑43 (MQ=255)                                                                                                                        gcagcTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTaa               >  2:966346/1‑129 (MQ=255)                                                                                                                        gcagcTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTaa               <  1:966346/129‑1 (MQ=255)                                                                                                                           gcTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAAtt  >  2:324068/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                CAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATT  >  NC_000913/923960‑924220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑