| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 989,969 | T→C | S130G (AGT→GGT) | pncB ← | nicotinate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 989,969 | 0 | T | C | 97.3% | 136.9 / NA | 37 | S130G (AGT→GGT) | pncB | nicotinate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (15/21); minor base A (0/1); total (15/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAA > NC_000913/989846‑990095 | gATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCaa < 1:243309/139‑1 (MQ=255) aaGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGaa > 2:127465/1‑139 (MQ=255) aaGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACAGATGACCGCCAGCAAAGGaa < 2:1216028/139‑1 (MQ=255) aGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGCCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGAAc < 1:1422115/139‑1 (MQ=255) gcggTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCAc > 1:1266447/1‑139 (MQ=255) gcggTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCAc > 2:470882/1‑139 (MQ=255) aaCGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACg > 1:1374474/1‑139 (MQ=255) agaAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCt < 2:210721/139‑1 (MQ=255) aaGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTa < 2:431889/139‑1 (MQ=255) tCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAAc > 2:786906/1‑139 (MQ=255) tAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGAAGTTCCCCGAGGATGACTTCACGCCACGGGCCGCTTAAACGAata < 1:1008853/139‑1 (MQ=255) tttCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCtt < 1:470882/139‑1 (MQ=255) tCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGc < 2:1258961/139‑1 (MQ=255) aGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGcc > 1:1097068/1‑97 (MQ=255) aGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGcc < 2:1097068/97‑1 (MQ=255) ttGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACg < 1:539241/139‑1 (MQ=255) ttGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACg < 1:707285/139‑1 (MQ=255) ttGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACg < 1:786906/139‑1 (MQ=255) tGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCt > 2:154274/1‑101 (MQ=255) tGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCt < 1:154274/101‑1 (MQ=255) gcgcAACGTCGGCCTGCGGTGAGCGATAGTGATGTACCATTTCACCGATAACCGCCAGCaa < 1:429518/61‑1 (MQ=255) gcgcAACGTCGGCCTGCGGTGAGCGATAGTGATGTACCATTTCACCGATAACCGCCAGCaa > 2:429518/1‑61 (MQ=255) cgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGc > 1:797259/1‑54 (MQ=255) cgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGc < 2:797259/54‑1 (MQ=255) cGGTGAGCGATAGCGATGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTa > 1:422081/1‑139 (MQ=255) agcgatagcgatGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCg > 2:1498417/1‑139 (MQ=255) cgatGTACCATTTCACCGATAACCGCCAGCAAAGGaa < 2:259906/37‑1 (MQ=255) cgatGTACCATTTCACCGATAACCGCCAGCAAAGGaa > 1:259906/1‑37 (MQ=37) atGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt < 1:1027193/92‑1 (MQ=255) atGTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt > 2:1027193/1‑92 (MQ=255) gTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAc < 2:259997/109‑1 (MQ=255) gTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAc > 1:259997/1‑109 (MQ=255) gTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa < 1:769145/139‑1 (MQ=255) gTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa < 1:1498417/139‑1 (MQ=255) gTACCATTTCACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa < 1:127465/139‑1 (MQ=255) cACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa > 1:816372/1‑130 (MQ=255) cACCGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa < 2:816372/130‑1 (MQ=255) | GATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAA > NC_000913/989846‑990095 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |