Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,121,934	A→G	I225T (ATC→ACC)	pyrC ←	dihydro‑orotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,121,934	0	A	G	100.0%	196.7 / NA	51	I225T (ATC→ACC)	pyrC	dihydro‑orotase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/29);  total (22/29)

GAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTG  >  NC_000913/1121798‑1122068                                                                                                                                         |                                                                                                                                       gAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTg                                                                                                                                      >  1:1306822/1‑139 (MQ=255)  aaGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTgg                                                                                                                                     <  2:91041/139‑1 (MQ=255)  aaGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTgg                                                                                                                                     <  2:605617/139‑1 (MQ=255)     cagcCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTa                                                                                                                                  <  2:815954/139‑1 (MQ=255)     cagcCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTa                                                                                                                                  <  1:1229656/139‑1 (MQ=255)                               tttGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGTTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCt                                                                                                        <  2:1239598/139‑1 (MQ=255)                               tttGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCt                                                                                                        <  1:766233/139‑1 (MQ=255)                                   cGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCaa                                                                                                    <  2:1306822/139‑1 (MQ=255)                                    gATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAAc                                                                                                   <  1:1057898/139‑1 (MQ=255)                                    gATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAAc                                                                                                   <  1:732799/139‑1 (MQ=255)                                    gATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAAc                                                                                                   <  1:1446073/139‑1 (MQ=255)                                         cGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCt                                                                                                        <  2:235911/129‑1 (MQ=255)                                         cGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCt                                                                                                        >  1:235911/1‑129 (MQ=255)                                         cGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCa                                                                                              >  1:592378/1‑139 (MQ=255)                                               tgtgGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGt                                                                                        <  2:592378/139‑1 (MQ=255)                                                    cgcAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGacgcacgc                                                                                                          <  2:469246/116‑1 (MQ=255)                                                    cgcAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGacgcacgc                                                                                                          >  1:469246/1‑116 (MQ=255)                                                      cAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGtt                                                                                 >  1:465766/1‑139 (MQ=255)                                                           tCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACACAATGCCTGTTGGTGAATATTACGTTTGAGGGTgg                                                                                                                                     <  2:1112783/82‑1 (MQ=255)                                                           tCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACACAATGCCTGTTGGTGAATATTACGTTTGAGGGTgg                                                                                                                                     >  1:1112783/1‑82 (MQ=255)                                                                aCCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGa                                                                       <  2:159925/139‑1 (MQ=255)                                                                aCCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTAGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGa                                                                       <  1:154203/139‑1 (MQ=255)                                                                   gAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGAtgc                                                                    >  1:115894/1‑139 (MQ=255)                                                                    aGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAAc                                                                                                   >  2:1184582/1‑107 (MQ=255)                                                                    aGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAAc                                                                                                   <  1:1184582/107‑1 (MQ=255)                                                                                   aaaCCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCt                                                                                                        >  1:1127407/1‑87 (MQ=255)                                                                                   aaaCCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCt                                                                                                        <  2:1127407/87‑1 (MQ=255)                                                                                        gCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGcagcc                                               >  1:1085257/1‑139 (MQ=255)                                                                                         cTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAAc                                                                                                   <  1:725276/86‑1 (MQ=255)                                                                                         cTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAAc                                                                                                   >  2:725276/1‑86 (MQ=255)                                                                                         cTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCg                                                                <  2:1454273/121‑1 (MQ=255)                                                                                         cTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCg                                                                >  1:1454273/1‑121 (MQ=255)                                                                                         cTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGcagcca                                              <  2:5026/139‑1 (MQ=255)                                                                                                      cACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTcc                                 <  2:465766/139‑1 (MQ=255)                                                                                                         gCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTc                              >  1:125450/1‑139 (MQ=255)                                                                                                             tGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCa                             <  1:835920/136‑1 (MQ=255)                                                                                                             tGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCa                             >  2:835920/1‑136 (MQ=255)                                                                                                             tGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACgg                          >  1:1072539/1‑139 (MQ=255)                                                                                                             tGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACgg                          >  1:1473116/1‑139 (MQ=255)                                                                                                                  gTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGt                                                                                  <  2:521867/78‑1 (MQ=255)                                                                                                                  gTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGt                                                                                  >  1:521867/1‑78 (MQ=255)                                                                                                                   ttGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAg                    >  2:823149/1‑139 (MQ=255)                                                                                                                       tGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCgg                >  1:315576/1‑139 (MQ=255)                                                                                                                         aaTATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGgca              <  1:1142458/139‑1 (MQ=255)                                                                                                                         aaTATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGgca              >  2:1142458/1‑139 (MQ=255)                                                                                                                         aaTATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGgca              <  2:1085257/139‑1 (MQ=255)                                                                                                                                cGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCtt       <  2:651898/139‑1 (MQ=255)                                                                                                                                     gAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAAc                                                                                                   <  2:158943/42‑1 (MQ=255)                                                                                                                                     gAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAAc                                                                                                   >  1:158943/1‑42 (MQ=255)                                                                                                                                     gAGGGTGGGTAGACAATACAGGTGCGGAAGCACGCCTCCAACCAGCATATGGTTGCGGTTAACCATCCGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTtggtg  >  2:880307/1‑139 (MQ=255)                                                                                                                                      aGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGcagccag                                             <  1:1383090/95‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTG  >  NC_000913/1121798‑1122068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑