Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	61,437	C→T	V610M (GTG→ATG)	rapA ←	RNA polymerase remodeling/recycling factor ATPase, RNA polymerase‑associated, ATP‑dependent RNA translocase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	61,437	0	C	T	100.0%	261.5 / NA	75	V610M (GTG→ATG)	rapA	RNA polymerase remodeling/recycling factor ATPase, RNA polymerase‑associated, ATP‑dependent RNA translocase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/38);  total (37/38)

CCAGATAGTTAATCAGATCGTTGTATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCACATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGA  >  NC_000913/61302‑61571                                                                                                                                        |                                                                                                                                       ccAGATAGTTAATCAGATCGTTGTATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATg                                                                                                                                     <  1:451931/139‑1 (MQ=255) ccAGATAGTTAATCAGATCGTTGTATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATg                                                                                                                                     <  1:82803/139‑1 (MQ=255)                 aTCGTTGTATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCATACCTTCGTGATACCAGCGCACCAGCACCGACTTATCGGTTTTCTCCAGATAAGGCATATGTATCTGAATATCGTgc                                                                                                                     >  2:1366424/1‑139 (MQ=255)                 aTCGTTGTATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATg                                                                                                                                     <  2:894646/123‑1 (MQ=255)                 aTCGTTGTATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATg                                                                                                                                     >  1:894646/1‑123 (MQ=255)                 aTCGTTGTATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTgc                                                                                                                     >  1:1247577/1‑139 (MQ=255)                 aTCGTTGTATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTgc                                                                                                                     >  2:365117/1‑139 (MQ=255)                             gCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATa                                                                                                         <  2:1370369/139‑1 (MQ=255)                               tATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACGAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATg                                                                                                                                     >  1:853940/1‑109 (MQ=255)                               tATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATg                                                                                                                                     <  2:853940/109‑1 (MQ=255)                               tATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACg                                                                                                       <  1:365117/139‑1 (MQ=255)                               tATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACg                                                                                                       >  1:1307509/1‑139 (MQ=255)                                aTCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGa                                                                                                      <  1:1366424/139‑1 (MQ=255)                                aTCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGa                                                                                                      >  2:1273595/1‑139 (MQ=255)                                 tCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGAt                                                                                                     >  2:106498/1‑139 (MQ=255)                                 tCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAACGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGAt                                                                                                     >  2:1434870/1‑139 (MQ=255)                                  cGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATa                                                                                                         >  2:1015154/1‑134 (MQ=255)                                  cGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATc                                                                                                    >  1:184227/1‑139 (MQ=255)                                  cGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATc                                                                                                    <  2:857440/139‑1 (MQ=255)                                  cGTAAATAGTGCGGCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATa                                                                                                         <  1:1015154/134‑1 (MQ=255)                                         aGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGa                                                                                                                                   >  2:484891/1‑101 (MQ=255)                                         aGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGa                                                                                                                                   <  1:484891/101‑1 (MQ=255)                                           tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATg                                                                                                                                     >  2:700434/1‑97 (MQ=255)                                           tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATg                                                                                                                                     <  1:700434/97‑1 (MQ=255)                                           tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGaa                                                                                                                             <  1:1480002/105‑1 (MQ=255)                                           tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGaa                                                                                                                             <  2:1324878/105‑1 (MQ=255)                                           tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGaa                                                                                                                             >  1:1324878/1‑105 (MQ=255)                                           tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGaa                                                                                                                             >  2:1480002/1‑105 (MQ=255)                                           tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCg                                                                                                                        <  2:469646/110‑1 (MQ=255)                                           tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCg                                                                                                                        >  1:919344/1‑110 (MQ=255)                                           tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCg                                                                                                                        <  2:919344/110‑1 (MQ=255)                                           tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCg                                                                                                                        >  1:469646/1‑110 (MQ=255)                                           tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGa                                                                                                           >  2:1200304/1‑123 (MQ=255)                                           tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGa                                                                                                           <  1:1200304/123‑1 (MQ=255)                                           tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGAcc                                                                                           >  1:622174/1‑139 (MQ=255)                                           tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGAcc                                                                                           >  1:589241/1‑139 (MQ=255)                                           tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGAcc                                                                                           >  1:573490/1‑139 (MQ=255)                                           tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGAcc                                                                                           >  2:835635/1‑139 (MQ=255)                                           tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGAcc                                                                                           >  2:1049595/1‑139 (MQ=255)                                           tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGAcc                                                                                           >  1:551669/1‑139 (MQ=255)                                            gCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCa                                                                                          <  2:551669/139‑1 (MQ=255)                                            gCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCa                                                                                          >  2:1416943/1‑139 (MQ=255)                                             cGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATg                                                                                                                                     <  1:733710/95‑1 (MQ=255)                                             cGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATg                                                                                                                                     >  2:733710/1‑95 (MQ=255)                                                ccGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATAc                                                                                      <  1:1049595/139‑1 (MQ=255)                                                 cGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACg                                                                                     <  2:278039/139‑1 (MQ=255)                                                  ggTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACgc                                                                                    <  1:1274469/139‑1 (MQ=255)                                                     cGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACgctgc                                                                                 <  1:835635/139‑1 (MQ=255)                                                         cAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCa                                                                             <  2:173363/139‑1 (MQ=255)                                                          aGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAg                                                                            <  2:1035001/139‑1 (MQ=255)                                                               tgCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAgac                                                                                               >  2:1141279/1‑115 (MQ=255)                                                               tgCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAgac                                                                                               <  1:1141279/115‑1 (MQ=255)                                                                 cTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATcc                                                                     <  1:1416943/139‑1 (MQ=255)                                                                 cTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATcc                                                                     >  2:1412465/1‑139 (MQ=255)                                                                 cTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATcc                                                                     >  1:932651/1‑139 (MQ=255)                                                                         cATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGaa                                                             >  2:1166276/1‑139 (MQ=255)                                                                         cATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGaa                                                             <  1:1074847/139‑1 (MQ=255)                                                                            ccAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATgg                                                          <  2:622174/139‑1 (MQ=255)                                                                             cAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGc                                                         <  2:589241/139‑1 (MQ=255)                                                                                 ccTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGt                                                     <  2:1307509/139‑1 (MQ=255)                                                                                  cTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAACGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTc                                                    <  1:1434870/139‑1 (MQ=255)                                                                                           aCCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGAGCCTGGCCGATACGATCCAGACGACCAATACgc                                                                                    <  2:1367270/98‑1 (MQ=255)                                                                                           aCCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGAGCCTGGCCGATACGATCCAGACGACCAATACgc                                                                                    >  1:1367270/1‑98 (MQ=255)                                                                                                cgcACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGc                                                                                                              >  2:336041/1‑67 (MQ=255)                                                                                                cgcACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGc                                                                                                              <  1:336041/67‑1 (MQ=255)                                                                                                    ccAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCg                                                                    <  2:363426/105‑1 (MQ=255)                                                                                                    ccAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCg                                                                    >  1:363426/1‑105 (MQ=255)                                                                                                       gCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCAc                                           <  1:466794/127‑1 (MQ=255)                                                                                                       gCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCAc                                           >  2:466794/1‑127 (MQ=255)                                                                                                                 aGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAg                     <  1:106498/139‑1 (MQ=255)                                                                                                                  gCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGt                    >  2:467430/1‑139 (MQ=255)                                                                                                                      ttttCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACg                <  2:1247577/139‑1 (MQ=255)                                                                                                                                  aaGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAgac                                                                                               >  1:333497/1‑48 (MQ=255)                                                                                                                                  aaGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAgac                                                                                               <  2:333497/48‑1 (MQ=255)                                                                                                                                    ggCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGa  >  2:1148789/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       CCAGATAGTTAATCAGATCGTTGTATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCACATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGA  >  NC_000913/61302‑61571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑