Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,390,113	A→G	C162C (TGT→TGC)	mpaA ←	murein peptide amidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,390,113	0	A	G	100.0%	194.4 / NA	52	C162C (TGT→TGC)	mpaA	murein peptide amidase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/25);  total (27/25)

TGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGC  >  NC_000913/1389985‑1390247                                                                                                                                 |                                                                                                                                       tgcaaGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTg                                                                                                                              >  2:205039/1‑139 (MQ=255)                gCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTgg                                                                                                                             >  1:692277/1‑125 (MQ=255)                gCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTgg                                                                                                                             <  2:692277/125‑1 (MQ=255)                     ccAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGaccacc                                                                                                          <  1:205039/139‑1 (MQ=255)                      cAACTGCCGAAAGAGCCAGGCGTTTCATGACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCa                                                                                                                                      <  2:247734/110‑1 (MQ=255)                      cAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCa                                                                                                                                      >  1:247734/1‑110 (MQ=255)                        aCTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCcag                                                                                                       >  1:987166/1‑139 (MQ=255)                         cTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTgg                                                                                                                             >  2:848653/1‑116 (MQ=255)                         cTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTgg                                                                                                                             <  1:848653/116‑1 (MQ=255)                                    gCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATg                                                                                           <  2:348444/139‑1 (MQ=255)                                                    ccGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGta                                                                                    <  1:193425/130‑1 (MQ=255)                                                    ccGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGta                                                                                    >  2:193425/1‑130 (MQ=255)                                                       aCGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATg                                                                                           <  1:1207885/120‑1 (MQ=255)                                                       aCGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATg                                                                                           >  2:1207885/1‑120 (MQ=255)                                                       aCGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGcaca                                                                        >  2:648110/1‑139 (MQ=255)                                                                aCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTggg                                                               >  1:1201918/1‑139 (MQ=255)                                                                       gCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCa                                                                                                                                      <  2:99109/61‑1 (MQ=255)                                                                       gCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGac                                                                                                              >  2:306490/1‑85 (MQ=255)                                                                       gCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGac                                                                                                              <  1:306490/85‑1 (MQ=255)                                                                       gCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAgg                                                        >  2:1260739/1‑139 (MQ=255)                                                                           ttCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCg                                                    >  2:681495/1‑139 (MQ=255)                                                                                 cGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCa                                              >  2:1296682/1‑139 (MQ=255)                                                                                   ccTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAgg                                            <  1:966181/139‑1 (MQ=255)                                                                                      gAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGttt                                         >  1:515448/1‑139 (MQ=255)                                                                                       agccagccATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTg                                        >  2:921885/1‑139 (MQ=255)                                                                                       agccagccATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGATGTATAAGCTGGCACAGCGCCTGGGTTTCAGGtt                                                      <  1:291902/125‑1 (MQ=255)                                                                                       agccagccATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGATGTATAAGCTGGCACAGCGCCTGGGTTTCAGGtt                                                      >  2:291902/1‑125 (MQ=255)                                                                                            gccaTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCAcc                                   >  1:793498/1‑139 (MQ=255)                                                                                             ccaTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGac                                                                                                              <  2:187361/63‑1 (MQ=255)                                                                                             ccaTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGac                                                                                                              >  1:187361/1‑63 (MQ=255)                                                                                             ccaTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGcc                                               <  1:1222017/126‑1 (MQ=255)                                                                                             ccaTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGcc                                               >  2:1222017/1‑126 (MQ=255)                                                                                             ccaTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCt                                  <  2:1224040/139‑1 (MQ=255)                                                                                              caTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTg                                 >  2:665344/1‑139 (MQ=255)                                                                                               aTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGt                                <  1:648110/139‑1 (MQ=255)                                                                                                ttCGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTc                               <  1:1296682/139‑1 (MQ=255)                                                                                                  cGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGc                                                                                          <  1:129560/78‑1 (MQ=255)                                                                                                  cGCCTAATTCGCTGTGTCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAg                             <  2:515448/139‑1 (MQ=255)                                                                                                  cGCCTAATTCGCTGTGTCTGGGATATTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGc                                                                                          >  2:129560/1‑78 (MQ=255)                                                                                                               gtgtCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTc                               >  1:1167947/1‑124 (MQ=255)                                                                                                               gtgtCTGGGATCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTc                               <  2:1167947/124‑1 (MQ=255)                                                                                                                         tCTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAg      <  2:1201918/139‑1 (MQ=255)                                                                                                                          cTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGcc                                                                   <  2:497425/77‑1 (MQ=255)                                                                                                                          cTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGcc                                                                   >  1:497425/1‑77 (MQ=255)                                                                                                                          cTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGtt                                                      >  2:201568/1‑90 (MQ=255)                                                                                                                          cTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGtt                                                      <  1:201568/90‑1 (MQ=255)                                                                                                                          cTTCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAgc     >  2:595645/1‑139 (MQ=255)                                                                                                                            tCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTc                                                           <  2:88973/83‑1 (MQ=255)                                                                                                                            tCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTc                                                           >  1:88973/1‑83 (MQ=255)                                                                                                                            tCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAgcgg   >  1:134923/1‑139 (MQ=255)                                                                                                                            tCAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAgcgg   <  1:921885/139‑1 (MQ=255)                                                                                                                             cAATGCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAgcggc  <  1:1260739/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                       TGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGC  >  NC_000913/1389985‑1390247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑