| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,475,008 | A→G | E263G (GAG→GGG) | ydbC → | putative NAD(P)‑binding oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,475,008 | 0 | A | G | 98.6% | 264.8 / ‑3.5 | 71 | coding (788/861 nt) | ydbC | putative NAD(P)‑binding oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (31/39); minor base . (1/0); total (32/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
CTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCCTTTTTTTCTTTTTTAATGGCATGGAGTGC > NC_000913/1474872‑1475141 | cTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGggga > 2:602374/1‑139 (MQ=255) cAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAata > 1:589427/1‑139 (MQ=255) cAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAata > 1:1205882/1‑139 (MQ=255) aaTCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAatat < 2:1291876/139‑1 (MQ=255) acacTTTCCGATGTTTCTGCGAGCCTGGGTGCATCACCAATGCAGGTGTCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATTCCAGGGACGTCTTCTGTTGCGCATTTACGGGGGAATATGGctgctg < 2:1297899/139‑1 (MQ=255) acacTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGctgctg < 2:67390/139‑1 (MQ=255) cacTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGa < 1:1397459/139‑1 (MQ=255) tCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTACGCATTTACGGGGGAATATGGCTGCTGAAAAAt > 2:92832/1‑139 (MQ=255) aTGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCa < 1:1164498/139‑1 (MQ=255) ttGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCt < 1:602374/139‑1 (MQ=255) ctgcGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTc > 2:1239278/1‑139 (MQ=255) ctgcGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTc > 2:735894/1‑139 (MQ=255) gAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAg < 2:1205882/139‑1 (MQ=255) tGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAgt < 1:1297968/139‑1 (MQ=255) tGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAgt < 1:1448061/139‑1 (MQ=255) gggTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAgtg < 2:589427/139‑1 (MQ=255) acacCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTa > 1:382631/1‑139 (MQ=255) acCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACg < 1:1180444/139‑1 (MQ=255) cAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGtt > 2:1280457/1‑139 (MQ=255) gTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTa > 2:1020751/1‑139 (MQ=255) ggCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTAtt > 1:1216092/1‑139 (MQ=255) cTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTcgc < 2:1216092/139‑1 (MQ=255) cTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTcgc < 1:1239278/139‑1 (MQ=255) ggCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATa < 1:1020751/139‑1 (MQ=255) cTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAAc < 2:70248/139‑1 (MQ=255) cTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAAc < 1:795340/139‑1 (MQ=255) cTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAAc < 1:799258/139‑1 (MQ=255) gTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGa < 2:382631/139‑1 (MQ=255) gTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGa < 1:1172445/139‑1 (MQ=255) tACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCa > 1:185223/1‑85 (MQ=255) tACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCa < 2:185223/85‑1 (MQ=255) cGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCAt < 1:853759/81‑1 (MQ=255) cGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCAt > 2:853759/1‑81 (MQ=255) ttCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACaaa < 2:1248681/139‑1 (MQ=255) ttGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATgg > 1:818927/1‑49 (MQ=255) ttGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATgg < 2:818927/49‑1 (MQ=255) ttGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCa > 2:276028/1‑139 (MQ=255) tGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCAt > 2:310392/1‑139 (MQ=255) gCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTcgcg > 2:1481208/1‑105 (MQ=255) gCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTcgcg < 1:1481208/105‑1 (MQ=255) cGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAgtg < 1:1413569/65‑1 (MQ=255) cGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAgtg > 2:1413569/1‑65 (MQ=255) cGTCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCCTTTTTTTCtt > 2:162934/1‑139 (MQ=255) tCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAAtt < 2:369263/44‑1 (MQ=255) tCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAAtt > 1:369263/1‑44 (MQ=255) tCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCa > 2:336932/1‑47 (MQ=255) tCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCa < 1:661322/47‑1 (MQ=255) tCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCa > 2:661322/1‑47 (MQ=255) tCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCa < 1:336932/47‑1 (MQ=255) tCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGtgttgt < 1:1060771/67‑1 (MQ=255) tCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGtgttgt > 2:1060771/1‑67 (MQ=255) tCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGAt < 2:1293847/78‑1 (MQ=255) tCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGAt > 1:1293847/1‑78 (MQ=255) tCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAAt < 2:767529/94‑1 (MQ=255) tCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAAt > 1:767529/1‑94 (MQ=255) tCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGa > 1:970014/1‑113 (MQ=255) tCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGa < 2:970014/113‑1 (MQ=255) tCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCCTTTTTTTCtttt > 2:285367/1‑139 (MQ=255) tCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCCTTTTTTTCtttt > 1:932162/1‑139 (MQ=255) tCTTCGGTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCCTTTTTTTCtttt > 1:1075176/1‑139 (MQ=255) tCTTCGGTTGCGCATTTACGGG‑GAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCCTTTTTTTCttttt > 2:1445666/1‑139 (MQ=255) gTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATgg < 2:285902/74‑1 (MQ=255) gTTGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATgg > 1:285902/1‑74 (MQ=255) tGCGCATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCCTTTTTTTCTTTTTTAATGGc < 1:285367/139‑1 (MQ=255) gcgcATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAgg > 2:384239/1‑49 (MQ=255) gcgcATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAgg < 1:384239/49‑1 (MQ=255) gcgcATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCCTTTTTTTCTTTTTTAATGGCa < 1:92832/139‑1 (MQ=255) cATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTcgcg < 2:1332271/77‑1 (MQ=255) cATTTACGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTcgcg > 1:1332271/1‑77 (MQ=255) aCGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGa < 1:627871/60‑1 (MQ=255) aCGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGa > 2:627871/1‑60 (MQ=255) aCGGGGGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCCTTTTTTTCTTTTTTAATGGCATGGAGTGc < 1:735894/139‑1 (MQ=255) | CTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCCTTTTTTTCTTTTTTAATGGCATGGAGTGC > NC_000913/1474872‑1475141 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |