Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,306,934 A→G intergenic (‑180/+38) mqo ← / ← yojI malate dehydrogenase, FAD/NAD(P)‑binding domain/microcin J25 efflux ABC transporter permease/ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,306,9340AG95.9% 174.3 / ‑0.2 49intergenic (‑180/+38)mqo/yojImalate dehydrogenase, FAD/NAD(P)‑binding domain/microcin J25 efflux ABC transporter permease/ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (31/16);  minor base T (1/1);  total (32/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

TAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCG  >  NC_000913/2306826‑2307068
                                                                                                            |                                                                                                                                      
tAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGcgt                                                                                                          <  1:421320/139‑1 (MQ=255)
  acaacaTTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGcgtcg                                                                                                        <  1:906822/139‑1 (MQ=255)
                           ttATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACgg                                                                               >  2:883526/1‑139 (MQ=255)
                                        ttAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGc                                                                  >  1:507332/1‑139 (MQ=255)
                                          aGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCgg                                                                >  1:531350/1‑139 (MQ=255)
                                                          cGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGcc                                                >  1:834460/1‑139 (MQ=255)
                                                            ttGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg                                                                                                             <  2:1399776/76‑1 (MQ=255)
                                                            ttGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg                                                                                                             >  1:1399776/1‑76 (MQ=255)
                                                               attaattaTAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCa                                           <  2:834460/139‑1 (MQ=255)
                                                                         gggTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAg                                                                   <  2:1168020/105‑1 (MQ=255)
                                                                         gggTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAg                                                                   >  1:1168020/1‑105 (MQ=255)
                                                                         gggTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAg                                 >  2:84295/1‑139 (MQ=255)
                                                                              attaGAAATGAAGGTATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg                                                                                                             >  1:916378/1‑58 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg                                                                                                             >  1:248842/1‑58 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg                                                                                                             <  2:1109033/58‑1 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg                                                                                                             <  1:1280449/58‑1 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg                                                                                                             <  2:916378/58‑1 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg                                                                                                             <  2:248842/58‑1 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg                                                                                                             >  2:1280449/1‑58 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg                                                                                                             >  1:1109033/1‑58 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGcgtcg                                                                                                        >  2:760950/1‑63 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGcgtcg                                                                                                        <  1:760950/63‑1 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGc                                                                              <  2:10172/89‑1 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGc                                                                              >  1:10172/1‑89 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc                            >  2:415652/1‑139 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc                            >  2:494238/1‑139 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc                            >  2:1356666/1‑139 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc                            >  2:730493/1‑139 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc                            >  2:960439/1‑139 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc                            >  1:102219/1‑139 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc                            >  1:596652/1‑139 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc                            >  1:518236/1‑139 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc                            >  1:1116429/1‑139 (MQ=255)
                                                                              attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTACGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc                            >  1:1457279/1‑139 (MQ=255)
                                                                               ttaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCg                                                                 <  2:396195/101‑1 (MQ=255)
                                                                               ttaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCg                                                                 >  1:396195/1‑101 (MQ=255)
                                                                               ttaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGccc                           >  1:867573/1‑139 (MQ=255)
                                                                                 aGAAATGAAGGGATTTTTAGCATATTGTCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCAt                         >  1:400376/1‑139 (MQ=255)
                                                                                     aTGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATcgcg                                                         <  2:450144/103‑1 (MQ=255)
                                                                                     aTGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATcgcg                                                         >  1:450144/1‑103 (MQ=255)
                                                                                        aaGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCAt                  >  1:1304356/1‑139 (MQ=255)
                                                                                          gggATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATAGTAGGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCAttt                >  2:996280/1‑139 (MQ=255)
                                                                                                tttAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTgcg                     >  2:1164927/1‑128 (MQ=255)
                                                                                                tttAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTgcg                     <  1:1164927/128‑1 (MQ=255)
                                                                                                     cATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCgg     <  2:1044700/139‑1 (MQ=255)
                                                                                                     cATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCgg     >  2:53274/1‑139 (MQ=255)
                                                                                                      atatTGTCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGt    <  2:400376/139‑1 (MQ=255)
                                                                                                      atatTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGt    <  2:518236/139‑1 (MQ=255)
                                                                                                        atTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCg  >  1:211365/1‑139 (MQ=255)
                                                                                                            |                                                                                                                                      
TAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCG  >  NC_000913/2306826‑2307068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: