Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,306,934 | A→G | intergenic (‑180/+38) | mqo ← / ← yojI | malate dehydrogenase, FAD/NAD(P)‑binding domain/microcin J25 efflux ABC transporter permease/ATPase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,306,934 | 0 | A | G | 95.9% | 174.3 / ‑0.2 | 49 | intergenic (‑180/+38) | mqo/yojI | malate dehydrogenase, FAD/NAD(P)‑binding domain/microcin J25 efflux ABC transporter permease/ATPase |
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (31/16); minor base T (1/1); total (32/17) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01 |
TAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCG > NC_000913/2306826‑2307068 | tAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGcgt < 1:421320/139‑1 (MQ=255) acaacaTTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGcgtcg < 1:906822/139‑1 (MQ=255) ttATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACgg > 2:883526/1‑139 (MQ=255) ttAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGc > 1:507332/1‑139 (MQ=255) aGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCgg > 1:531350/1‑139 (MQ=255) cGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGcc > 1:834460/1‑139 (MQ=255) ttGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg < 2:1399776/76‑1 (MQ=255) ttGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg > 1:1399776/1‑76 (MQ=255) attaattaTAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCa < 2:834460/139‑1 (MQ=255) gggTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAg < 2:1168020/105‑1 (MQ=255) gggTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAg > 1:1168020/1‑105 (MQ=255) gggTTATTAGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAg > 2:84295/1‑139 (MQ=255) attaGAAATGAAGGTATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg > 1:916378/1‑58 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg > 1:248842/1‑58 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg < 2:1109033/58‑1 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg < 1:1280449/58‑1 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg < 2:916378/58‑1 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg < 2:248842/58‑1 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg > 2:1280449/1‑58 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAg > 1:1109033/1‑58 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGcgtcg > 2:760950/1‑63 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGcgtcg < 1:760950/63‑1 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGc < 2:10172/89‑1 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGc > 1:10172/1‑89 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc > 2:415652/1‑139 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc > 2:494238/1‑139 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc > 2:1356666/1‑139 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc > 2:730493/1‑139 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc > 2:960439/1‑139 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc > 1:102219/1‑139 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc > 1:596652/1‑139 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc > 1:518236/1‑139 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc > 1:1116429/1‑139 (MQ=255) attaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTACGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGcc > 1:1457279/1‑139 (MQ=255) ttaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCg < 2:396195/101‑1 (MQ=255) ttaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCg > 1:396195/1‑101 (MQ=255) ttaGAAATGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGccc > 1:867573/1‑139 (MQ=255) aGAAATGAAGGGATTTTTAGCATATTGTCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCAt > 1:400376/1‑139 (MQ=255) aTGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATcgcg < 2:450144/103‑1 (MQ=255) aTGAAGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATcgcg > 1:450144/1‑103 (MQ=255) aaGGGATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCAt > 1:1304356/1‑139 (MQ=255) gggATTTTTAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATAGTAGGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCAttt > 2:996280/1‑139 (MQ=255) tttAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTgcg > 2:1164927/1‑128 (MQ=255) tttAGCATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTgcg < 1:1164927/128‑1 (MQ=255) cATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCgg < 2:1044700/139‑1 (MQ=255) cATATTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCgg > 2:53274/1‑139 (MQ=255) atatTGTCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGt < 2:400376/139‑1 (MQ=255) atatTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGt < 2:518236/139‑1 (MQ=255) atTGGCCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCg > 1:211365/1‑139 (MQ=255) | TAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCG > NC_000913/2306826‑2307068 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |