Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,401,103 G→A T151T (ACC→ACT)  nuoF ← NADH:ubiquinone oxidoreductase, chain F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,401,1030GA100.0% 105.0 / NA 31T151T (ACC→ACT) nuoFNADH:ubiquinone oxidoreductase, chain F
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/18);  total (13/18)

GGTTAGCACGACGTCCTTCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCGGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGC  >  NC_000913/2400967‑2401232
                                                                                                                                        |                                                                                                                                 
ggTTAGCACGACGTCCTTCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGt                                                                                                                                 <  2:1504122/139‑1 (MQ=255)
           cgTCCTTCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCaa                                                                                                                      >  1:1090690/1‑139 (MQ=255)
                ttCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCa                                                                                                                 <  2:1250498/139‑1 (MQ=255)
                   cAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACgg                                                                                                              <  1:126935/139‑1 (MQ=255)
                                aaCGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTg                                                                                                 <  1:84633/139‑1 (MQ=255)
                                                 gCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCg                                                                                >  1:55125/1‑139 (MQ=255)
                                                  cAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGc                                                                               <  2:1091046/139‑1 (MQ=255)
                                                    gATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCa                                                                             <  1:1150773/139‑1 (MQ=255)
                                                          gcgcCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAgg                                                                       <  2:549112/139‑1 (MQ=255)
                                                          gcgcCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAgg                                                                       >  1:549112/1‑139 (MQ=255)
                                                           cgcCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGa                                                                      <  2:1173912/139‑1 (MQ=255)
                                                                     cGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAACAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGcc                                                            <  1:54254/139‑1 (MQ=255)
                                                                       gTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCa                                                                                                                 <  2:1057253/84‑1 (MQ=255)
                                                                       gTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCa                                                                                                                 >  1:1057253/1‑84 (MQ=255)
                                                                            gACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCa                                                                                                                 >  1:399399/1‑79 (MQ=255)
                                                                            gACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCa                                                                                                                 <  2:399399/79‑1 (MQ=255)
                                                                            gACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTaa                                                     >  1:1384292/1‑139 (MQ=255)
                                                                                 aCAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCttt                                                <  1:1083144/139‑1 (MQ=255)
                                                                                  cAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGACGCAGATTAACTGCCGCTTCGATATATTCGCCACg                                                                           <  1:342947/111‑1 (MQ=255)
                                                                                  cAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGACGCAGATTAACTGCCGCTTCGATATATTCGCCACg                                                                           >  2:342947/1‑111 (MQ=255)
                                                                                       cgaaatcgaaaCCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAgcgc                                          <  1:1099284/139‑1 (MQ=255)
                                                                                            tcgaaaCCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAAcg                                     <  2:1056629/139‑1 (MQ=255)
                                                                                                           aTAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATAc                      >  2:1293406/1‑139 (MQ=255)
                                                                                                                   tttGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAgg                                                                       >  1:374104/1‑82 (MQ=255)
                                                                                                                   tttGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAgg                                                                       <  2:374104/82‑1 (MQ=255)
                                                                                                                   tttGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTccacc              >  1:1227203/1‑139 (MQ=255)
                                                                                                                           gCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGa                                                                      <  1:557177/75‑1 (MQ=255)
                                                                                                                           gCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGa                                                                      >  2:557177/1‑75 (MQ=255)
                                                                                                                           gCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTg      >  2:718309/1‑139 (MQ=255)
                                                                                                                            cAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGc     <  2:336998/139‑1 (MQ=255)
                                                                                                                               aCCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGc  >  2:844525/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                 
GGTTAGCACGACGTCCTTCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCGGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGC  >  NC_000913/2400967‑2401232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑