Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,597,778	A→G	intergenic (‑160/+53)	purC ← / ← bamC	phosphoribosylaminoimidazole‑succinocarboxamide synthetase/BamABCDE complex OM biogenesis lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,597,778	0	A	G	100.0%	244.0 / NA	63	intergenic (‑160/+53)	purC/bamC	phosphoribosylaminoimidazole‑succinocarboxamide synthetase/BamABCDE complex OM biogenesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/36);  total (27/36)

GTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACACGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATG  >  NC_000913/2597646‑2597912                                                                                                                                     |                                                                                                                                       gTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTg                                                                                                                                  <  2:1191117/139‑1 (MQ=255) gTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTg                                                                                                                                  <  1:790708/139‑1 (MQ=255)             tttGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGttt                                                                                                                                   <  1:1020374/126‑1 (MQ=255)             tttGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGttt                                                                                                                                   >  2:1020374/1‑126 (MQ=255)                   gTCTTGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTACGTATCATATCAGaaaaa                                                                                                                <  1:993309/139‑1 (MQ=255)                    tCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGTGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGaaaaaa                                                                                                               >  1:1030368/1‑139 (MQ=255)                    tCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCCGCAGACGCACGCGTTTGCGTATCATATCAGaaaaaa                                                                                                               >  2:1045108/1‑139 (MQ=255)                        gtgtGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGc                                                                                                           <  1:1227908/139‑1 (MQ=255)                        gtgtGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGc                                                                                                           <  1:601238/139‑1 (MQ=255)                           tgCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCgg                                                                                                        >  2:952053/1‑139 (MQ=255)                             ccGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGta                                                                                                                              <  2:586397/115‑1 (MQ=255)                             ccGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGta                                                                                                                              >  1:586397/1‑115 (MQ=255)                             ccGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGgat                                                                                                      <  1:613535/139‑1 (MQ=255)                              cGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGga                                                                                                       >  2:1334489/1‑137 (MQ=255)                              cGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGga                                                                                                       <  1:1334489/137‑1 (MQ=255)                              cGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGAGTTTTCCTGCAGACGCACGCGTTTGCGTATAATATCAGAAAAAAGGGCCGgatg                                                                                                     >  2:993309/1‑139 (MQ=255)                                       tGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGcc                                                                                            <  1:952053/139‑1 (MQ=255)                                        gTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGccc                                                                                           <  2:1411140/139‑1 (MQ=255)                                        gTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGccc                                                                                           <  2:418859/139‑1 (MQ=255)                                            gATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTa                                                                                       >  2:1330982/1‑139 (MQ=255)                                              tGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGtat                                                                                                                             >  1:414304/1‑99 (MQ=255)                                              tGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGtat                                                                                                                             <  2:414304/99‑1 (MQ=255)                                                       aaCGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGc                                                                            >  1:681688/1‑139 (MQ=255)                                                        aCGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGTGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCt                                                                           <  2:1030368/139‑1 (MQ=255)                                                             ttATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACg                                                                      >  2:672537/1‑139 (MQ=255)                                                                         cGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGa                                                          >  1:1412139/1‑139 (MQ=255)                                                                          gtacGACAAACAAATTTCATGCGAATTACAGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTg                                                                                                                                  <  1:957879/63‑1 (MQ=255)                                                                          gTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTg                                                                                                                                  >  2:957879/1‑66 (MQ=255)                                                                          gTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGccc                                                                                           >  1:179693/1‑105 (MQ=255)                                                                          gTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGccc                                                                                           <  2:179693/105‑1 (MQ=255)                                                                          gTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGAc                                                         >  2:47203/1‑139 (MQ=255)                                                                          gTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGAc                                                         >  2:289870/1‑139 (MQ=255)                                                                           tCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACa                                                        >  2:176732/1‑139 (MQ=255)                                                                                   acaaaTTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTg                                                                                                                                  >  1:326179/1‑57 (MQ=255)                                                                                   acaaaTTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTg                                                                                                                                  <  2:326179/57‑1 (MQ=255)                                                                                    caaaTTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAgc                                               <  2:33248/139‑1 (MQ=255)                                                                                       aTTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGcgcg                                            <  1:1330982/139‑1 (MQ=255)                                                                                       aTTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGcgcg                                            <  1:176732/139‑1 (MQ=255)                                                                                        tttCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGcgt                                           <  1:672537/139‑1 (MQ=255)                                                                                        tttCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGcgt                                           <  1:47203/139‑1 (MQ=255)                                                                                           cGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATAACAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTA‑CAGCGCGTCGtt                                       <  1:1007283/139‑1 (MQ=255)                                                                                             tGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGgat                                                                                                      <  1:1151370/75‑1 (MQ=255)                                                                                             tGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGgat                                                                                                      >  2:1151370/1‑75 (MQ=255)                                                                                             tGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACTGCTACCAGCGCGTCGTTc                                      >  1:1013536/1‑139 (MQ=255)                                                                                                  aTTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGAct                                 <  2:1412139/139‑1 (MQ=255)                                                                                                  aTTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGAct                                 <  2:1215877/139‑1 (MQ=255)                                                                                                    tACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGActct                               <  1:1486446/139‑1 (MQ=255)                                                                                                        gCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGt                           <  2:603671/139‑1 (MQ=255)                                                                                                            aaaTCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAg                                                                                              >  2:195402/1‑68 (MQ=255)                                                                                                            aaaTCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAg                                                                                              <  1:195402/68‑1 (MQ=255)                                                                                                                cgcgTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTAt                   >  2:179395/1‑139 (MQ=255)                                                                                                                 gcgTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCt                                     >  2:212605/1‑120 (MQ=255)                                                                                                                 gcgTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCt                                     <  1:212605/120‑1 (MQ=255)                                                                                                                      ttCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGcc                                                                 >  1:1397485/1‑87 (MQ=255)                                                                                                                      ttCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGcc                                                                 <  2:1397485/87‑1 (MQ=255)                                                                                                                       tCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCttt            >  2:402724/1‑139 (MQ=255)                                                                                                                           gCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGa        <  1:1045108/139‑1 (MQ=255)                                                                                                                                cGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCGGAGTATGACCTTTCGGATCGAt   <  1:289870/139‑1 (MQ=255)                                                                                                                                 gCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACg                                                                      >  1:512651/1‑71 (MQ=255)                                                                                                                                 gCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACg                                                                      <  2:512651/71‑1 (MQ=255)                                                                                                                                 gCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGAct                                 >  2:711602/1‑108 (MQ=255)                                                                                                                                 gCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGAct                                 <  1:711602/108‑1 (MQ=255)                                                                                                                                 gCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATg  <  1:402724/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       GTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACACGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATG  >  NC_000913/2597646‑2597912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑