Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,899,230	T→C	V63V (GTA→GTG)	ygcU ←	putative FAD‑linked oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,899,230	0	T	C	100.0%	225.8 / NA	60	V63V (GTA→GTG)	ygcU	putative FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/29);  total (31/29)

TTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGA  >  NC_000913/2899098‑2899362                                                                                                                                     |                                                                                                                                     ttgattCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGc                                                                                                                                >  2:29596/1‑139 (MQ=255) ttgattCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGc                                                                                                                                >  2:475501/1‑139 (MQ=255) ttgattCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGc                                                                                                                                >  1:991374/1‑139 (MQ=255)    attCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGctct                                                                                                                             >  2:1341529/1‑139 (MQ=255)     ttCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCtg                                                                                                                            <  2:58071/139‑1 (MQ=255)              gAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGa                                                                                                                   >  2:832570/1‑139 (MQ=255)              gAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGa                                                                                                                   >  2:672171/1‑139 (MQ=255)               aGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAg                                                                                                                  >  1:842532/1‑139 (MQ=255)                    tCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTa                                                                                                             >  2:1221351/1‑139 (MQ=255)                        gCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTGTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGAc                                                                                                         >  1:930142/1‑139 (MQ=255)                         caccacCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGAcc                                                                                                        >  2:1197481/1‑139 (MQ=255)                              ccGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGc                                                                                                   >  2:568732/1‑139 (MQ=255)                                 aGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCgg                                                                                                >  2:486470/1‑139 (MQ=255)                                         acaacaGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCa                                                                                        <  1:1423085/139‑1 (MQ=255)                                         acaacaGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCa                                                                                        <  1:672171/139‑1 (MQ=255)                                          caacaGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCaa                                                                                       <  1:832570/139‑1 (MQ=255)                                             caGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGt                                                                                    >  2:581148/1‑139 (MQ=255)                                              aGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGta                                                                                   <  1:482567/139‑1 (MQ=255)                                               gTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGtat                                                                                  <  2:249913/139‑1 (MQ=255)                                                tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGtata                                                                                 >  2:777982/1‑139 (MQ=255)                                                tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGtata                                                                                 <  2:703729/139‑1 (MQ=255)                                                tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGtata                                                                                 <  1:1230716/139‑1 (MQ=255)                                                tttCCAGCCCACCTGCGGTGGCGGAAGCACCTGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTACTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGtata                                                                                 >  2:121977/1‑139 (MQ=255)                                                 ttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATaa                                                                                <  1:581148/139‑1 (MQ=255)                                                    cAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATg                                                                             <  2:553690/139‑1 (MQ=255)                                                          aCCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCa                                                                                        >  2:374558/1‑122 (MQ=255)                                                          aCCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCa                                                                                        <  1:374558/122‑1 (MQ=255)                                                                     cGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGaaa                                                            >  1:583380/1‑139 (MQ=255)                                                                           cACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTg                                                                                                                                 <  1:632879/64‑1 (MQ=255)                                                                           cACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTg                                                                                                                                 >  2:632879/1‑64 (MQ=255)                                                                              cGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGaaa                                                   <  1:683342/139‑1 (MQ=255)                                                                              cGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGaaa                                                   >  1:113549/1‑139 (MQ=255)                                                                                   cgcgGCACACCGTTAATTTTGTGCGCATCCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGt                                              >  1:399105/1‑139 (MQ=255)                                                                                           acCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGc                                                                                         <  2:1280563/88‑1 (MQ=255)                                                                                           acCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGc                                                                                         >  1:1280563/1‑88 (MQ=255)                                                                                           acCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATa                                         <  2:97099/136‑1 (MQ=255)                                                                                           acCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATa                                         >  1:97099/1‑136 (MQ=255)                                                                                             cGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGtt                                    >  2:65199/1‑139 (MQ=255)                                                                                              gTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                   <  1:777982/139‑1 (MQ=255)                                                                                              gTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                   <  1:1221351/139‑1 (MQ=255)                                                                                              gTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                   <  2:583380/139‑1 (MQ=255)                                                                                                tAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttttt                                 <  1:568732/139‑1 (MQ=255)                                                                                                       gtgCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAAt                                                                                             <  1:716410/72‑1 (MQ=255)                                                                                                       gtgCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAAt                                                                                             >  2:716410/1‑72 (MQ=255)                                                                                                       gtgCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGGTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAAt                          >  2:927378/1‑139 (MQ=255)                                                                                                       gtgCGCATTCATAAAATTCAGCACACGGGACACTTGCTCAGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAAt                          >  1:588770/1‑139 (MQ=255)                                                                                                        tgCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTTTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAAATTACGAAAACGGTCAATACTGttt                                   >  2:13196/1‑129 (MQ=255)                                                                                                        tgCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                   <  1:13196/129‑1 (MQ=255)                                                                                                            cATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGt                     <  1:121977/139‑1 (MQ=255)                                                                                                               tCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTc                  >  1:469215/1‑139 (MQ=255)                                                                                                                cATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCa                 <  1:29596/139‑1 (MQ=255)                                                                                                                 aTAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCAt                <  1:1197481/139‑1 (MQ=255)                                                                                                                  tAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATGCGGAAATTTCCGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATc               <  2:930142/139‑1 (MQ=255)                                                                                                                         cAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                   <  1:312404/112‑1 (MQ=255)                                                                                                                         cAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                   >  2:312404/1‑112 (MQ=255)                                                                                                                           gCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATaa                                                                                >  2:1200935/1‑65 (MQ=255)                                                                                                                           gCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATaa                                                                                <  1:1200935/65‑1 (MQ=255)                                                                                                                           gCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTAc      <  1:475501/139‑1 (MQ=255)                                                                                                                               acGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGa  <  1:1341529/139‑1 (MQ=255)                                                                                                                               acGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGa  >  2:1104132/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                     TTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGA  >  NC_000913/2899098‑2899362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑