Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,916,189	T→C	L378P (CTG→CCG)	barA →	hybrid sensory histidine kinase, in two‑component regulatory system with UvrY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,916,189	0	T	C	100.0%	218.7 / NA	57	L378P (CTG→CCG)	barA	hybrid sensory histidine kinase, in two‑component regulatory system with UvrY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/31);  total (26/31)

GATTGAACGTTCGGCAAATAATTTGCTGGCAATTATTAATGATGTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCTGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGACCCGCTGCGATTACAGCAAATCATCACTAACCTGGTGGGGAATGCAATTAAA  >  NC_000913/2916055‑2916322                                                                                                                                       |                                                                                                                                      gATTGAACGTTCGGCAAATAATTTGCTGGCAATTATTAATGATGTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTccgctg                                                                                                                                   <  2:1359418/139‑1 (MQ=255)   ttGAACGTTCGGCAAATAATTTGCTGGCAATTATTAATGATGTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGc                                                                                                                                 >  2:714533/1‑139 (MQ=255)        cGTTCGGCAAATAATTTGCTGGCAATTATTAATGATGTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACAtt                                                                                                                            <  2:1245436/139‑1 (MQ=255)              gCAAATAATTTGCTGGCAATTATTAATGATGTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTtctc                                                                                                                      <  2:644057/139‑1 (MQ=255)              gCAAATAATTTGCTGGCAATTATTAATGATGTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTtctc                                                                                                                      <  2:694782/139‑1 (MQ=255)              gCAAATAATTTGCTGGCAATTATTAATGATGTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTtctc                                                                                                                      <  1:652877/139‑1 (MQ=255)                     atTTGCTGGCAATTATTAATGATGTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATaa                                                                                                               <  2:358897/139‑1 (MQ=255)                     atTTGCTGGCAATTATTAATGATGTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATaa                                                                                                               <  1:1066133/139‑1 (MQ=255)                     atTTGCTGGCAATTATTAATGATGTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATaa                                                                                                               <  1:971345/139‑1 (MQ=255)                     atTTGCTGGCAATTATTAATGATGTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATaa                                                                                                               <  1:714533/139‑1 (MQ=255)                                attattAATGATGTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAAc                                                                                                    >  1:82624/1‑139 (MQ=255)                                    ttaATGATGTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGAc                                                                                                <  2:1046671/139‑1 (MQ=255)                                        tgatgTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTc                                                                                            <  2:992117/139‑1 (MQ=255)                                            gTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATaa                                                                                                               >  2:1313298/1‑116 (MQ=255)                                            gTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATaa                                                                                                               <  1:1313298/116‑1 (MQ=255)                                            gTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACg                                                                                               <  2:626181/132‑1 (MQ=255)                                            gTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACg                                                                                               >  1:626181/1‑132 (MQ=255)                                            gTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAata                                                                                        >  2:1130396/1‑139 (MQ=255)                                             ttCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAatat                                                                                       >  1:1209465/1‑139 (MQ=255)                                               ctcGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATaa                                                                                                               >  2:1264063/1‑113 (MQ=255)                                               ctcGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATaa                                                                                                               <  1:1264063/113‑1 (MQ=255)                                                tcGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTg                                                                                                  <  1:926365/125‑1 (MQ=255)                                                tcGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTg                                                                                                  >  2:926365/1‑125 (MQ=255)                                                     ttCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCg                                                                               <  1:819806/139‑1 (MQ=255)                                                       ctcGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGTCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGAc                                                                             >  2:775479/1‑139 (MQ=255)                                                       ctcGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGAc                                                                             >  2:1460238/1‑139 (MQ=255)                                                        tcGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACg                                                                            <  2:82624/139‑1 (MQ=255)                                                        tcGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACg                                                                            <  2:1160551/139‑1 (MQ=255)                                                                       ggTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGa                                                             <  1:1207053/139‑1 (MQ=255)                                                                         tAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAAATGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATc                                                           >  2:553773/1‑139 (MQ=255)                                                                           aGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCgg                                                         >  2:83837/1‑139 (MQ=255)                                                                            gCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGc                                                        <  1:553773/139‑1 (MQ=255)                                                                                 ttCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGAccc                                                   <  1:1130396/139‑1 (MQ=255)                                                                                    tGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGACCCGCt                                                <  2:1067304/139‑1 (MQ=255)                                                                                           tATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGACCCGCTGCGATTa                                         >  1:610209/1‑139 (MQ=255)                                                                                              tccattccCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATaa                                                                                                               >  2:1232666/1‑66 (MQ=255)                                                                                              tccattccCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATaa                                                                                                               <  1:1232666/66‑1 (MQ=255)                                                                                              tccattccCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGACCCGCTGCGATTACAg                                      >  2:1276140/1‑139 (MQ=255)                                                                                               ccattccCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGACCCGCTGCGATTACAGc                                     <  2:612636/139‑1 (MQ=255)                                                                                                  ttccCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGACCCGCTGCGATTACAGCaaa                                  >  2:872309/1‑139 (MQ=255)                                                                                                   tccCACTACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGACCCGCTGCGATTACAGCAAat                                 <  1:872309/139‑1 (MQ=255)                                                                                                         tACGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGACCCGCTGCGATTACAGCAAATCATCAc                           >  2:815145/1‑139 (MQ=255)                                                                                                          aCGCAGCACGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGACCCGCTGCGATTACAGCAAATCATCACt                          >  2:1235481/1‑139 (MQ=255)                                                                                                            gcagcaCGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTc                                                                                            >  1:1318427/1‑71 (MQ=255)                                                                                                            gcagcaCGCTGGATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTc                                                                                            <  2:1318427/71‑1 (MQ=255)                                                                                                                       gATGAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGACCCGCTGCGATTACAGCAAATCATCACTAACCTGGTGGGGa             >  1:361724/1‑139 (MQ=255)                                                                                                                          gAAGTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGACCCGCTGCGATTACAGCAAATCATCACTAACCTGGTGGGGAATg          >  1:1399173/1‑139 (MQ=255)                                                                                                                             gTCGTTACTCCGCTGGCACATTCTTCTCACGATaa                                                                                                               <  2:1100877/35‑1 (MQ=255)                                                                                                                             gTCGTTACTCCGCTGGCACATTCTTCTCACGATaa                                                                                                               >  1:1100877/1‑35 (MQ=255)                                                                                                                             gTCGTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGACCCGCTGCGATTACAGCAAATCATCACTAACCTGGTGGGGAATGCaa       >  1:560869/1‑139 (MQ=255)                                                                                                                                gTTACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGACCCGCTGCGATTACAGCAAATCATCACTAACCTGGTGGGGAATGCAATTa    <  1:1460238/139‑1 (MQ=255)                                                                                                                                 ttACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCATTATTAATAGCGACGTGCCTGATCACGTGATCGGCGACCCGCTGCGATTACAg                                      <  2:871438/104‑1 (MQ=255)                                                                                                                                 ttACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGt                                                                           >  2:423645/1‑67 (MQ=255)                                                                                                                                 ttACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGt                                                                           <  1:423645/67‑1 (MQ=255)                                                                                                                                 ttACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGACCCGCTGCGATTAc                                        >  2:232365/1‑102 (MQ=255)                                                                                                                                 ttACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGACCCGCTGCGATTAc                                        <  1:232365/102‑1 (MQ=255)                                                                                                                                 ttACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGACCCGCTGCGATTACAg                                      >  1:871438/1‑104 (MQ=255)                                                                                                                                  tACTCCGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGACCCGCTGCGATTACAGCAAATCATCACTAACCTGGTGGGGAATGCAATTaaa  <  1:1276140/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      GATTGAACGTTCGGCAAATAATTTGCTGGCAATTATTAATGATGTTCTCGACTTCTCGAAACTGGAAGCAGGTAAGCTGATTCTGGAAAGTATTCCATTCCCACTACGCAGCACGCTGGATGAAGTCGTTACTCTGCTGGCACATTCTTCTCACGATAAAGGGTTAGAACTGACGCTCAATATTAAAAGCGACGTGCCTGATAACGTGATCGGCGACCCGCTGCGATTACAGCAAATCATCACTAACCTGGTGGGGAATGCAATTAAA  >  NC_000913/2916055‑2916322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑