Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,081,402	G→A	T78T (ACC→ACT)	tktA ←	transketolase 1, thiamine triphosphate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,081,402	0	G	A	100.0%	233.0 / NA	66	T78T (ACC→ACT)	tktA	transketolase 1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/32);  total (34/32)

CTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCGGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGTCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTC  >  NC_000913/3081266‑3081533                                                                                                                                         |                                                                                                                                    cTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGTGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGt                                                                                                                                   <  1:341801/139‑1 (MQ=255)          cAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcag                                                                                                                          <  1:697754/139‑1 (MQ=255)          cAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcag                                                                                                                          <  2:495497/139‑1 (MQ=255)          cAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcag                                                                                                                          <  2:912315/139‑1 (MQ=255)           aaTACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcagc                                                                                                                         <  1:87793/139‑1 (MQ=255)            aTACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcagca                                                                                                                        <  1:649960/139‑1 (MQ=255)            aTACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcagca                                                                                                                        <  2:716751/139‑1 (MQ=255)              aCCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAgg                                                                                                                      >  2:96913/1‑139 (MQ=255)               cccTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGc                                                                                                                     <  1:913451/139‑1 (MQ=255)                   gACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTg                                                                                                                   >  2:86875/1‑137 (MQ=255)                   gACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTg                                                                                                                   <  1:86875/137‑1 (MQ=255)                   gACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTa                                                                                                                 >  2:393540/1‑139 (MQ=255)                         gCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcagca                                                                                                                        >  1:1361428/1‑126 (MQ=255)                         gCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcagca                                                                                                                        <  2:1361428/126‑1 (MQ=255)                         gCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAg                                                                                                           >  2:919834/1‑139 (MQ=255)                              ccGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATgg                                                                                                      <  1:919834/139‑1 (MQ=255)                              ccGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATgg                                                                                                      >  1:1416859/1‑139 (MQ=255)                               cGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGc                                                                                                                     >  1:953025/1‑123 (MQ=255)                               cGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGc                                                                                                                     <  2:953025/123‑1 (MQ=255)                                    gtggtTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGt                                                                                                >  1:845064/1‑139 (MQ=255)                                           ccACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGtt                                                                                         >  2:1320847/1‑139 (MQ=255)                                                 cAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAg                                                                                   >  2:487778/1‑139 (MQ=255)                                                 cAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTC‑TCCATCGGCAGATCGTAACCAGTGAGGTgcag                                                                                                                          <  1:1387918/99‑1 (MQ=255)                                                 cAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTC‑TCCATCGGCAGATCGTAACCAGTGAGGTgcag                                                                                                                          >  2:1387918/1‑99 (MQ=255)                                                   gCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcag                                                                                                                          <  1:601012/98‑1 (MQ=255)                                                   gCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcag                                                                                                                          >  2:601012/1‑98 (MQ=255)                                                      gtgtAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGa                                                                              >  1:1498428/1‑139 (MQ=255)                                                        gtAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcagca                                                                                                                        >  1:131279/1‑95 (MQ=255)                                                        gtAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcagca                                                                                                                        <  2:131279/95‑1 (MQ=255)                                                         tAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcagca                                                                                                                        >  2:437128/1‑94 (MQ=255)                                                         tAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcagca                                                                                                                        <  1:437128/94‑1 (MQ=255)                                                              cACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGcggtca                                                                      >  2:1447287/1‑139 (MQ=255)                                                                 ttCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcag                                                                                                                          <  2:242309/84‑1 (MQ=255)                                                                 ttCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcag                                                                                                                          >  1:242309/1‑84 (MQ=255)                                                                 ttCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAgg                                                                                                                      >  1:1129291/1‑88 (MQ=255)                                                                 ttCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAgg                                                                                                                      <  2:1129291/88‑1 (MQ=255)                                                                 ttCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGcggtcacgg                                                                   >  2:850091/1‑139 (MQ=255)                                                                  tCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGcggtcacggt                                                                  <  2:1498428/139‑1 (MQ=255)                                                                  tCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGcggtcacggt                                                                  <  2:203470/139‑1 (MQ=255)                                                                   ccGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGcggtcacggtc                                                                 >  1:64815/1‑139 (MQ=255)                                                                      ggTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcagca                                                                                                                        <  2:888566/81‑1 (MQ=255)                                                                      ggTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcagca                                                                                                                        >  1:888566/1‑81 (MQ=255)                                                                      ggTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGTCAGc                                                              >  2:458227/1‑139 (MQ=255)                                                                           cccGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGc                                                                                                   >  1:1312657/1‑97 (MQ=255)                                                                           cccGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGc                                                                                                   <  2:1312657/97‑1 (MQ=255)                                                                           cccGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGTCAGCCCAgg                                                         >  1:495910/1‑139 (MQ=255)                                                                                 gTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGTCAGCCCAGGACGGAt                                                   <  2:1416859/139‑1 (MQ=255)                                                                                     tAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcagca                                                                                                                        <  2:484409/66‑1 (MQ=255)                                                                                     tAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgcagca                                                                                                                        >  1:484409/1‑66 (MQ=255)                                                                                           gCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGTCAGCCCAGGACGGATTCTGCGGGtt                                         <  2:845064/139‑1 (MQ=255)                                                                                            cAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTg                                                                                                                   >  1:1355094/1‑64 (MQ=255)                                                                                            cAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTg                                                                                                                   <  2:1355094/64‑1 (MQ=255)                                                                                            cAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGcggtcacggt                                                                  <  1:852687/113‑1 (MQ=255)                                                                                            cAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGcggtcacggt                                                                  >  2:852687/1‑113 (MQ=255)                                                                                            cAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGTCAGCCCAGGACGGATTCTGCGGGTtg                                        >  2:434576/1‑139 (MQ=255)                                                                                                 gACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGTCAGCCCAGGACGGATTCTGCGGGTTGTGttt                                   <  1:850091/139‑1 (MQ=255)                                                                                                         tttttCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgca                                                                                                                           <  2:1094963/43‑1 (MQ=255)                                                                                                         tttttCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTgca                                                                                                                           >  1:1094963/1‑43 (MQ=255)                                                                                                         tttttCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGTGGTCACGGTCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAAt                           >  2:111254/1‑139 (MQ=255)                                                                                                           tttCAGTTCTTCCATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGTCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCa                         >  1:602147/1‑139 (MQ=255)                                                                                                                      ccATCGGCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGTCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGAc              >  1:851934/1‑139 (MQ=255)                                                                                                                           ggCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGTCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCgg         <  2:602147/139‑1 (MQ=255)                                                                                                                           ggCAGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGTCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCgg         <  1:1447287/139‑1 (MQ=255)                                                                                                                              aGATCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGTCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCaa      <  1:393540/139‑1 (MQ=255)                                                                                                                                aTCGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGTCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATg    >  1:1293071/1‑139 (MQ=255)                                                                                                                                  cGTAACCAGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGTCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTc  >  2:606018/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                    CTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCGGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGTCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTC  >  NC_000913/3081266‑3081533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑