Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,189,993 A→G E38G (GAG→GGG)  yqiH → putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,189,9930AG100.0% 324.7 / NA 84E38G (GAG→GGG) yqiHputative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/38);  total (46/38)

AATTAATTCAGGATGAAACTAATGCGCTACTTAAATACAAAGAATATCATTGCAGCAGGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTTCCTCCAATGGTGCGT  >  NC_000913/3189860‑3190126
                                                                                                                                     |                                                                                                                                     
aattaattCAGGATGAAACTAATGCGCTACTTAAATACAAAGAATATCATTGCAGCAGGAGTATTACTCTCCTGTAGGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAg                                                                                                                                  >  2:1080023/1‑139 (MQ=255)
          ggATGAAACTAATGCGCTACTTAAATACAAAGAATATCATTGCAGCAGGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGa                                                                                                                        <  1:1000821/139‑1 (MQ=255)
                aaCTAATGCGCTACTTAAATACAAAGAATATCATTGCAGCAGGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCAtt                                                                                                                  >  2:1000215/1‑139 (MQ=255)
                   tAATGCGCTACTTAAATACAAAGAATATCATTGCAGCAGGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTaaa                                                                                                               <  2:335338/139‑1 (MQ=255)
                             cTTAAATACAAAGAATATCATTGCAGCAGGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAAt                                                                                                     >  2:937492/1‑139 (MQ=255)
                                       aaGAATATCATTGCAGCAGGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaa                                                                                           >  1:328856/1‑139 (MQ=255)
                                       aaGAATATCATTGCAGCAGGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaa                                                                                           >  1:1224460/1‑139 (MQ=255)
                                       aaGAATATCATTGCAGCAGGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaa                                                                                           >  1:1271278/1‑139 (MQ=255)
                                       aaGAATATCATTGCAGCAGGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaa                                                                                           >  2:725196/1‑139 (MQ=255)
                                        aGAATATCATTGCAGCAGGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaaa                                                                                          <  1:1091803/139‑1 (MQ=255)
                                              acaatGCAGCAAGAATATTAATCTCCTTTATGAGCAGCATTGGCTTGGGTTCCATTAACCCGGATCGTTCCCCTAATAAAATTAATGGGTCAAGTAAAGGCGAAACAATAAAACCTACCAATCAAAGTAAAAACCTGcc                                                                                    <  2:264555/135‑1 (MQ=255)
                                                   gcagcaGGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATc                                                                               <  1:1068793/139‑1 (MQ=255)
                                                    cagcagGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCt                                                                              >  2:36633/1‑139 (MQ=255)
                                                    cagcagGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCt                                                                              >  2:669531/1‑139 (MQ=255)
                                                    cagcagGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCt                                                                              >  1:883574/1‑139 (MQ=255)
                                                         ggAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGccc                                                                         <  1:669531/139‑1 (MQ=255)
                                                             tATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAAc                                                                     <  1:1046431/139‑1 (MQ=255)
                                                                  ctctCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGa                                                                <  1:725196/139‑1 (MQ=255)
                                                                   tctcCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGAt                                                               <  1:1000215/139‑1 (MQ=255)
                                                                    ctcCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATa                                                              >  2:842552/1‑139 (MQ=255)
                                                                     tcCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATag                                                             >  2:1014058/1‑139 (MQ=255)
                                                                       cTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGa                                                                <  1:762903/134‑1 (MQ=255)
                                                                       cTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGa                                                                >  2:762903/1‑134 (MQ=255)
                                                                       cTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATagaa                                                           >  2:467275/1‑139 (MQ=255)
                                                                          tATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCg                                                                                                                         <  1:110027/74‑1 (MQ=255)
                                                                          tATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCg                                                                                                                         >  2:110027/1‑74 (MQ=255)
                                                                          tATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGcc                                                                          >  2:1483846/1‑121 (MQ=255)
                                                                          tATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGcc                                                                          <  1:1483846/121‑1 (MQ=255)
                                                                          tATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGa                                                                >  2:1178116/1‑131 (MQ=255)
                                                                          tATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGa                                                                <  1:1178116/131‑1 (MQ=255)
                                                                          tATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGAt                                                        >  1:1378895/1‑139 (MQ=255)
                                                                          tATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGAt                                                        >  1:33572/1‑139 (MQ=255)
                                                                            tGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATAc                                                      >  1:704922/1‑139 (MQ=255)
                                                                               gcagcaTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGa                                                                <  2:260527/126‑1 (MQ=255)
                                                                               gcagcaTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGa                                                                >  1:260527/1‑126 (MQ=255)
                                                                               gcagcaTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGaa                                                   <  2:1271278/139‑1 (MQ=255)
                                                                               gcagcaTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGaa                                                   <  1:1014058/139‑1 (MQ=255)
                                                                               gcagcaTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGaa                                                   >  1:1347269/1‑139 (MQ=255)
                                                                                 agcaTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAg                                                 >  1:1331299/1‑139 (MQ=255)
                                                                                 agcaTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAg                                                 >  1:1426455/1‑139 (MQ=255)
                                                                                 agcaTTGCCTGGGGAGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCAGTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAg                                                 >  1:916747/1‑139 (MQ=255)
                                                                                   caTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGa                                                                >  2:1471625/1‑122 (MQ=255)
                                                                                   caTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGa                                                                <  1:1471625/122‑1 (MQ=255)
                                                                                             ggTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATc                                                                                                    >  1:1367409/1‑76 (MQ=255)
                                                                                             ggTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATc                                                                                                    <  2:1367409/76‑1 (MQ=255)
                                                                                             ggTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACTTGcc                                                                                    <  1:1460925/92‑1 (MQ=255)
                                                                                             ggTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACTTGcc                                                                                    >  2:1460925/1‑92 (MQ=255)
                                                                                             ggTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATcgc                                     >  1:1211822/1‑139 (MQ=255)
                                                                                              gTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATcgcg                                    <  2:1331299/139‑1 (MQ=255)
                                                                                               tGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATc                                                                               <  1:398784/95‑1 (MQ=255)
                                                                                               tGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATc                                                                               >  2:398784/1‑95 (MQ=255)
                                                                                                 ccATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGa                                 >  2:623259/1‑139 (MQ=255)
                                                                                                 ccATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGa                                 >  1:900519/1‑139 (MQ=255)
                                                                                                 ccATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGa                                 >  1:1340618/1‑139 (MQ=255)
                                                                                                 ccATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGa                                 >  2:82995/1‑139 (MQ=255)
                                                                                                 ccATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGa                                 >  2:1108097/1‑139 (MQ=255)
                                                                                                  catcatCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCa                                                                                                   <  2:959674/72‑1 (MQ=255)
                                                                                                  catcatCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCa                                                                                                   >  1:959674/1‑72 (MQ=255)
                                                                                                  catcatCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATc                                                                               <  2:1300224/92‑1 (MQ=255)
                                                                                                  catcatCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATc                                                                               >  1:1300224/1‑92 (MQ=255)
                                                                                                  catcatCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGAc                                >  2:1016326/1‑139 (MQ=255)
                                                                                                       tcCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTAt                           <  2:1347269/139‑1 (MQ=255)
                                                                                                            gATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCa                      <  2:1378895/139‑1 (MQ=255)
                                                                                                             aTCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCAc                     <  2:1211822/139‑1 (MQ=255)
                                                                                                                gTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGt                  <  2:328856/139‑1 (MQ=255)
                                                                                                                gTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCAAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGt                  <  2:33572/139‑1 (MQ=255)
                                                                                                                 tACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAAc                                                                     <  2:1311558/87‑1 (MQ=255)
                                                                                                                 tACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAAc                                                                     >  1:1311558/1‑87 (MQ=255)
                                                                                                                 tACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGtt                 >  1:340930/1‑139 (MQ=255)
                                                                                                                 tACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGAGACTTTATTGTCACTGtt                 >  1:1013088/1‑139 (MQ=255)
                                                                                                                  aCCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGcc                                                                          >  2:833701/1‑81 (MQ=255)
                                                                                                                  aCCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGcc                                                                          <  1:833701/81‑1 (MQ=255)
                                                                                                                  aCCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCaaa                                                                      >  1:1236690/1‑85 (MQ=255)
                                                                                                                  aCCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCaaa                                                                      <  2:1236690/85‑1 (MQ=255)
                                                                                                                  aCCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTtc                >  1:69181/1‑139 (MQ=255)
                                                                                                                       tATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTTCCTCCa           >  1:1409034/1‑139 (MQ=255)
                                                                                                                        aTCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTTCCTCCaa          >  1:10343/1‑139 (MQ=255)
                                                                                                                            tAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTTCCTCCAATGGt      <  1:1016326/139‑1 (MQ=255)
                                                                                                                            tAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTTCCTCCAATGGt      <  1:36633/139‑1 (MQ=255)
                                                                                                                            tAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTTCCTCCAATGGt      <  1:937492/139‑1 (MQ=255)
                                                                                                                            tAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTTCCTCCAATGGt      <  2:900519/139‑1 (MQ=255)
                                                                                                                            tAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTTCCTCCAATGGt      <  2:1426455/139‑1 (MQ=255)
                                                                                                                            tAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTTCCTCCAATGGt      <  2:1409034/139‑1 (MQ=255)
                                                                                                                            tAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTTCCTCCAATGGt      <  2:1340618/139‑1 (MQ=255)
                                                                                                                              atgaatggGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaaa                                                                                          <  1:867883/53‑1 (MQ=255)
                                                                                                                              atgaatggGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaaa                                                                                          >  2:867883/1‑53 (MQ=255)
                                                                                                                                gaatggGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTTCCTCCAATGGTGCGt  <  2:1224460/139‑1 (MQ=255)
                                                                                                                                 aatggGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACa                                                                    <  1:292636/72‑1 (MQ=255)
                                                                                                                                 aatggGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACa                                                                    >  2:292636/1‑72 (MQ=255)
                                                                                                                                 aatggGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATa                                                              >  1:28784/1‑78 (MQ=255)
                                                                                                                                 aatggGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATa                                                              <  2:28784/78‑1 (MQ=255)
                                                                                                                                  atggGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCAc                     >  2:1097792/1‑118 (MQ=255)
                                                                                                                                  atggGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCAc                     <  1:1097792/118‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
AATTAATTCAGGATGAAACTAATGCGCTACTTAAATACAAAGAATATCATTGCAGCAGGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTTCCTCCAATGGTGCGT  >  NC_000913/3189860‑3190126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: