Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,274,204	G→A	A235V (GCA→GTA)	garP ←	putative (D)‑galactarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,274,204	0	G	A	100.0%	234.5 / NA	66	A235V (GCA→GTA)	garP	putative (D)‑galactarate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/36);  total (30/36)

TGCACCAGATAAATCGGGAACCAGGTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGC  >  NC_000913/3274072‑3274329                                                                                                                                     |                                                                                                                              tGCACCAGATAAATCGGGAACCAGGTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCg                                                                                                                         <  2:813405/139‑1 (MQ=255)        gATAAATCGGGAACCAGGTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGcc                                                                                                                  >  2:1106846/1‑139 (MQ=255)        gATAAATCGGGAACCAGGTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGcc                                                                                                                  <  1:398898/139‑1 (MQ=255)                 ggAACCAGGTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCttttt                                                                                                         <  2:1127577/139‑1 (MQ=255)                 ggAACCAGGTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCttttt                                                                                                         >  2:856722/1‑139 (MQ=255)                  gAACCAGGTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGc                                                                                                              >  2:165515/1‑133 (MQ=255)                  gAACCAGGTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGc                                                                                                              <  1:165515/133‑1 (MQ=255)                        ggTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTcc                                                                                                  <  2:73463/139‑1 (MQ=255)                        ggTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTcc                                                                                                  <  1:319794/139‑1 (MQ=255)                             ggAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTtgtg                                                                                                      <  1:440709/130‑1 (MQ=255)                             ggAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTtgtg                                                                                                      >  2:440709/1‑130 (MQ=255)                                   aCCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGaccac                                                                                       >  2:505301/1‑139 (MQ=255)                                    ccAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGaccacc                                                                                      >  1:1268318/1‑139 (MQ=255)                                              gtgtTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCAtttt                                                                            <  1:856722/139‑1 (MQ=255)                                              gtgtTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCAtttt                                                                            <  1:126238/139‑1 (MQ=255)                                              gtgtTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCAtttt                                                                            <  2:97261/139‑1 (MQ=255)                                                  tGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCaga                                                                        >  2:762135/1‑139 (MQ=255)                                                        aaTATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCAtttt                                                                            <  1:205363/129‑1 (MQ=255)                                                        aaTATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCAtttt                                                                            >  2:205363/1‑129 (MQ=255)                                                           atTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCag                                                                         >  1:1382126/1‑129 (MQ=255)                                                           atTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCag                                                                         <  2:1382126/129‑1 (MQ=255)                                                             tGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCa                                                             >  1:1018631/1‑139 (MQ=255)                                                              gTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAg                                                            >  2:180280/1‑139 (MQ=255)                                                              gTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCATGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAg                                                            >  2:1064452/1‑139 (MQ=255)                                                               tCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGGTAAACTTCAGc                                                           >  1:978858/1‑139 (MQ=255)                                                                ccGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGct                                                          <  2:1295651/139‑1 (MQ=255)                                                                     aaaTACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTcc                                                     <  2:482702/139‑1 (MQ=255)                                                                     aaaTACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTcc                                                     <  2:978858/139‑1 (MQ=255)                                                                      aaTACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCg                                                    <  1:762135/139‑1 (MQ=255)                                                                         aCGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAg                                                 <  1:1106846/139‑1 (MQ=255)                                                                              cAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATa                                            <  2:788574/139‑1 (MQ=255)                                                                              cAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATa                                            >  1:1267813/1‑139 (MQ=255)                                                                                 catcatGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATa                                            <  2:543773/136‑1 (MQ=255)                                                                                 catcatGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATa                                            >  1:543773/1‑136 (MQ=255)                                                                                 catcatGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGt                                         <  1:1288126/139‑1 (MQ=255)                                                                                      tGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCat                                                                                                >  1:1114655/1‑79 (MQ=255)                                                                                      tGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCat                                                                                                <  2:1114655/79‑1 (MQ=255)                                                                                        cGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGAt                                  <  2:1268318/139‑1 (MQ=255)                                                                                             agagagCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTc                             >  1:1075834/1‑139 (MQ=255)                                                                                                  gCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGtt                        <  2:1018631/139‑1 (MQ=255)                                                                                                  gCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGtt                        <  1:799103/139‑1 (MQ=255)                                                                                                   cAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTc                                                                           <  2:137134/87‑1 (MQ=255)                                                                                                   cAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTc                                                                           >  1:137134/1‑87 (MQ=255)                                                                                                   cAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTa                       >  2:1239691/1‑139 (MQ=255)                                                                                                     aTTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATg                     >  2:692551/1‑139 (MQ=255)                                                                                                       tGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGaa                   <  2:1267813/139‑1 (MQ=255)                                                                                                         cTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATc                 >  1:591021/1‑139 (MQ=255)                                                                                                                   tGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCttttt                                                                                                         >  2:285456/1‑41 (MQ=255)                                                                                                                   tGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCttttt                                                                                                         <  1:1496811/41‑1 (MQ=255)                                                                                                                   tGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCttttt                                                                                                         <  1:285456/41‑1 (MQ=255)                                                                                                                   tGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCttttt                                                                                                         >  2:1496811/1‑41 (MQ=255)                                                                                                                   tGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACcgc                                                                                    <  2:438271/62‑1 (MQ=255)                                                                                                                   tGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACcgc                                                                                    >  1:438271/1‑62 (MQ=255)                                                                                                                        tttGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGt                                                                                                    <  1:1329305/41‑1 (MQ=255)                                                                                                                        tttGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGt                                                                                                    >  2:1329305/1‑41 (MQ=255)                                                                                                                        tttGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCg                                                    <  2:295727/89‑1 (MQ=255)                                                                                                                        tttGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCg                                                    >  1:295727/1‑89 (MQ=255)                                                                                                                        tttGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAAt                  <  2:382924/123‑1 (MQ=255)                                                                                                                        tttGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAAt                  >  1:382924/1‑123 (MQ=255)                                                                                                                        tttGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAgc  <  1:692551/139‑1 (MQ=255)                                                                                                                          tGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAg                                                            <  2:730165/79‑1 (MQ=255)                                                                                                                          tGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAg                                                            >  1:730165/1‑79 (MQ=255)                                                                                                                          tGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGt                                     >  2:430962/1‑102 (MQ=255)                                                                                                                          tGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGt                                     <  1:430962/102‑1 (MQ=255)                                                                                                                             gTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTcc                                                     <  2:771834/83‑1 (MQ=255)                                                                                                                             gTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTcc                                                     >  1:771834/1‑83 (MQ=255)                                                                                                                                     |                                                                                                                              TGCACCAGATAAATCGGGAACCAGGTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGC  >  NC_000913/3274072‑3274329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑