Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,304,862	G→A	A319V (GCT→GTT)	mtr ←	tryptophan transporter of high affinity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,304,862	0	G	A	100.0%	173.6 / NA	51	A319V (GCT→GTT)	mtr	tryptophan transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/25);  total (26/25)

CCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACG  >  NC_000913/3304728‑3304993                                                                                                                                         |                                                                                                                                    ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCg                                                                                                                                  >  2:1295457/1‑139 (MQ=255)       cAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAgtcgt                                                                                                                            <  2:787235/139‑1 (MQ=255)          ttGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGa                                                                                                                         >  2:570649/1‑139 (MQ=255)               gccCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCa                                                                                                                    >  2:1453597/1‑139 (MQ=255)               gccCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCa                                                                                                                    >  1:1355125/1‑139 (MQ=255)                      tGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGAt                                                                                                             <  2:1203413/139‑1 (MQ=255)                           gCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGcc                                                                                                        >  1:1275755/1‑139 (MQ=255)                               cTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGAt                                                                                                    >  2:480307/1‑139 (MQ=255)                               cTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGAt                                                                                                    >  2:914035/1‑139 (MQ=255)                                       gCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAg                                                                                                               <  1:1307840/120‑1 (MQ=255)                                       gCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAg                                                                                                               >  2:1307840/1‑120 (MQ=255)                                        cATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTcaaaca                                                                                           >  2:5191/1‑139 (MQ=255)                                            aCCAATGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAAcc                                                                                       >  2:1205975/1‑139 (MQ=255)                                                aTGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGc                                                                                   >  1:781268/1‑139 (MQ=255)                                                 tGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCg                                                                                  >  1:400045/1‑139 (MQ=255)                                                      tACAGGAGTCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACg                                                                             >  2:535435/1‑139 (MQ=255)                                                      tACAGGAATCCGTTCGGGAACAACAGCCCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTAc                                                                              >  2:820286/1‑139 (MQ=255)                                                      tACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAAcc                                                                                       >  1:1423562/1‑129 (MQ=255)                                                      tACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAAcc                                                                                       <  2:1423562/129‑1 (MQ=255)                                                       aCAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGc                                                                            >  2:1499765/1‑139 (MQ=255)                                                       aCAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGc                                                                            >  1:383779/1‑139 (MQ=255)                                                             aTCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGga                                                                      <  1:1205975/139‑1 (MQ=255)                                                             aTCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGga                                                                      <  1:5191/139‑1 (MQ=255)                                                              tCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCCAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCCAGTCGTCGAAACCAAACAGAGCTGCCAGATAGTCAAACAAACCCAGCGCTACGCCGAGgaa                                                                     >  2:1307028/1‑139 (MQ=255)                                                                cGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaacg                                                                   >  1:1260155/1‑139 (MQ=255)                                                                  ttCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaacgaa                                                                 >  2:1337421/1‑139 (MQ=255)                                                                   tCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaacgaac                                                                >  2:1475903/1‑139 (MQ=255)                                                                    cGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACt                                                               <  1:480307/139‑1 (MQ=255)                                                                      ggAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCg                                                             <  2:1260155/139‑1 (MQ=255)                                                                               gcccccccccACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCa                                                     <  2:1157669/136‑1 (MQ=255)                                                                               agccccccccACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCa                                                     <  1:1089553/136‑1 (MQ=255)                                                                               agccccccccACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCa                                                     <  1:1453597/136‑1 (MQ=255)                                                                               agccccccccACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCa                                                     <  1:1479377/136‑1 (MQ=255)                                                                                 ccccccccACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAg                                                                                                               <  2:1441321/78‑1 (MQ=255)                                                                                 ccccccccACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAg                                                                                                               >  1:1441321/2‑79 (MQ=255)                                                                                           aaCTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAac                                         >  1:378316/1‑139 (MQ=255)                                                                                            aCTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAaca                                        <  2:781268/139‑1 (MQ=255)                                                                                            aCTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAaca                                        <  1:1499765/139‑1 (MQ=255)                                                                                                  ggggCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACca                                  <  2:428076/139‑1 (MQ=255)                                                                                                     gCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACcagca                               >  2:1231335/1‑139 (MQ=255)                                                                                                      cAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACcagcag                              <  2:378316/139‑1 (MQ=255)                                                                                                      cAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACcagcag                              <  2:1275755/139‑1 (MQ=255)                                                                                                      cAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACcagcag                              <  1:914035/139‑1 (MQ=255)                                                                                                                 aaTGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGAc                   <  2:1355125/139‑1 (MQ=255)                                                                                                                   tGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTa                 <  2:400045/139‑1 (MQ=255)                                                                                                                   tGCCGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTa                 >  1:1299345/1‑139 (MQ=255)                                                                                                                      cGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGAcc                                   >  2:284961/1‑118 (MQ=255)                                                                                                                      cGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGAcc                                   <  1:284961/118‑1 (MQ=255)                                                                                                                      cGTTTTCAAGCGGCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACgg              <  2:1299345/139‑1 (MQ=255)                                                                                                                                  gCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTAcg  <  2:383779/139‑1 (MQ=255)                                                                                                                                  gCCCACAACCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTAcg  <  1:1337421/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    CCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACG  >  NC_000913/3304728‑3304993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑