Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,347,929	T→C	V272A (GTC→GCC)	yhbJ →	adaptor protein for GlmZ/GlmY sRNA decay, glucosamine‑6‑phosphate‑regulated, NTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,347,929	0	T	C	100.0%	293.5 / NA	78	V272A (GTC→GCC)	yhbJ	adaptor protein for GlmZ/GlmY sRNA decay, glucosamine‑6‑phosphate‑regulated, NTPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/38);  total (40/38)

AGCTATGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTG  >  NC_000913/3347794‑3348062                                                                                                                                         |                                                                                                                                      aGCTATGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCa                                                                                                                                    >  1:111210/1‑139 (MQ=255)     aTGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCa                                                                                                                                <  1:1160958/139‑1 (MQ=255)      tGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCCGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCAc                                                                                                                               >  1:691753/1‑139 (MQ=255)      tGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTccc                                                                                                                               >  2:892305/1‑137 (MQ=255)      tGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCAc                                                                                                                               >  1:963638/1‑139 (MQ=255)      tGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCAc                                                                                                                               >  2:1023162/1‑139 (MQ=255)      tGGTTACCTATGCTGGAAACCAAC‑ACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAg                                                                                                                                   <  1:1262037/134‑1 (MQ=255)      tGGTTACCTATGCTGGAAACCAAC‑ACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAg                                                                                                                                   >  2:1262037/1‑134 (MQ=255)       ggTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACg                                                                                                                              <  1:282159/139‑1 (MQ=255)        gTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGc                                                                                                                             >  1:133051/1‑139 (MQ=255)           aCCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCAt                                                                                                                          >  1:582725/1‑139 (MQ=255)           aCCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCAt                                                                                                                          >  2:1476515/1‑139 (MQ=255)           aCCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCAt                                                                                                                          >  1:1267673/1‑139 (MQ=255)             cTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCg                                                                                                                        <  2:1267673/139‑1 (MQ=255)                tGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTAc                                                                                                                     >  2:306276/1‑139 (MQ=255)                tGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTAc                                                                                                                     >  1:474271/1‑139 (MQ=255)                tGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTAc                                                                                                                     >  2:1359641/1‑139 (MQ=255)                        aCCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGaaa                                                                                                             <  1:219143/139‑1 (MQ=255)                          caacaaCCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGaaaaa                                                                                                           >  2:1374601/1‑139 (MQ=255)                                    aGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACCGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCa                                                                                                  >  1:1153373/1‑139 (MQ=255)                                    aGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCAt                                                                                                 <  1:1330394/139‑1 (MQ=255)                                            gACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGt                                                                                                                                  <  2:901652/98‑1 (MQ=255)                                            gACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGt                                                                                                                                  >  1:901652/1‑98 (MQ=255)                                              cGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAg                                                                                       >  2:343503/1‑139 (MQ=255)                                                gTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCa                                                                                     <  2:111210/139‑1 (MQ=255)                                                        tGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTg                                                                             <  1:1476515/139‑1 (MQ=255)                                                            tGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAAt                                                                         <  2:474271/139‑1 (MQ=255)                                                              tACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGt                                                                                           >  1:861524/1‑119 (MQ=255)                                                              tACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGt                                                                                           <  2:861524/119‑1 (MQ=255)                                                                  ggcggGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCTGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaaa                                                                   <  2:995435/139‑1 (MQ=255)                                                                  ggcggGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaaa                                                                   <  2:1488107/139‑1 (MQ=255)                                                                   gcggGAAACACCGTTCGGTGTATATTGCAGAGCAACTGGCATACTACTTCCTCTCGCGCAGTAAAAG‑CGCCCAGTCACGCCATCGTACGCCGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaaac                                                                  <  2:691753/139‑1 (MQ=255)                                                                    cggGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaaaca                                                                 >  2:1365512/1‑139 (MQ=255)                                                                    cggGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaaaca                                                                 >  2:255529/1‑139 (MQ=255)                                                                     gggAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACg                                                                                                         <  1:972335/98‑1 (MQ=255)                                                                     gggAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACg                                                                                                         >  2:972335/1‑98 (MQ=255)                                                                      ggAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAg                                                               <  2:133051/139‑1 (MQ=255)                                                                         aGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGaaa                                                                                                             >  1:1204135/1‑90 (MQ=255)                                                                         aGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGaaa                                                                                                             <  2:1204135/90‑1 (MQ=255)                                                                          gCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTgg                                                           <  2:963638/139‑1 (MQ=255)                                                                             ccGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACg                                                                                                         <  2:501781/90‑1 (MQ=255)                                                                             ccGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACg                                                                                                         >  1:501781/1‑90 (MQ=255)                                                                               gTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatg                                                      <  1:1085221/139‑1 (MQ=255)                                                                                ttCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaa                                                                    >  1:576481/1‑124 (MQ=255)                                                                                ttCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaa                                                                    <  2:576481/124‑1 (MQ=255)                                                                                     tgtATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGcc                                                <  1:1374601/139‑1 (MQ=255)                                                                                       tataTTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCg                                              <  1:1365512/139‑1 (MQ=255)                                                                                            tGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTg                                         >  1:212842/1‑139 (MQ=255)                                                                                               agagCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACTGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCaa                                      >  2:670174/1‑139 (MQ=255)                                                                                               agagCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCaa                                      >  1:1218283/1‑139 (MQ=255)                                                                                                 agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCCATg                                    >  2:107533/1‑139 (MQ=255)                                                                                                 agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATg                                    >  1:1441827/1‑139 (MQ=255)                                                                                                 agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATg                                    >  2:675027/1‑139 (MQ=255)                                                                                                   cAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGaa                                  >  1:633079/1‑139 (MQ=255)                                                                                                   cAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGaa                                  >  1:1251140/1‑139 (MQ=255)                                                                                                   cAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGaa                                  >  1:1238875/1‑139 (MQ=255)                                                                                                   cAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGaa                                  <  2:1238875/139‑1 (MQ=255)                                                                                                    aaCTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAg                                 <  2:1218283/139‑1 (MQ=255)                                                                                                    aaCTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAg                                 <  2:1346295/139‑1 (MQ=255)                                                                                                      cTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACg                                                                                                         >  1:776710/1‑65 (MQ=255)                                                                                                      cTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACg                                                                                                         <  2:776710/65‑1 (MQ=255)                                                                                                      cTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCt                               >  2:210535/1‑139 (MQ=255)                                                                                                         gCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGtt                            <  1:1265589/139‑1 (MQ=255)                                                                                                         gCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGtt                            <  1:306276/139‑1 (MQ=255)                                                                                                          cAGACTACTTCCGCTCGCGCGGTAAAAACCGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGtt                            <  2:1153373/139‑1 (MQ=255)                                                                                                              ctactTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAAt                       <  1:675027/139‑1 (MQ=255)                                                                                                                actTCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTa                     >  1:707223/1‑139 (MQ=255)                                                                                                                   tCCGCTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATg                  <  2:89265/139‑1 (MQ=255)                                                                                                                       cTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGaaa                                                                                                             <  2:142870/44‑1 (MQ=255)                                                                                                                       cTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGaaa                                                                                                             >  1:142870/1‑44 (MQ=255)                                                                                                                       cTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCaaa                                                                                   >  1:347351/1‑70 (MQ=255)                                                                                                                       cTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCaaa                                                                                   <  2:347351/70‑1 (MQ=255)                                                                                                                       cTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaaa                                                                   >  2:774398/1‑86 (MQ=255)                                                                                                                       cTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaaa                                                                   <  1:774398/86‑1 (MQ=255)                                                                                                                       cTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTggg                                                          <  2:225822/95‑1 (MQ=255)                                                                                                                       cTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTggg                                                          >  1:225822/1‑95 (MQ=255)                                                                                                                       cTCGCGCGGTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAgg              >  1:280364/1‑139 (MQ=255)                                                                                                                              ggTAAAAA‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGa       <  1:162642/139‑1 (MQ=255)                                                                                                                                   aaa‑CGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTg  <  1:754460/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      AGCTATGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAA‑CGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTG  >  NC_000913/3347794‑3348062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑