Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,450,674	G→A	P231S (CCA→TCA)	rplB ←	50S ribosomal subunit protein L2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,450,674	0	G	A	100.0%	177.2 / NA	51	P231S (CCA→TCA)	rplB	50S ribosomal subunit protein L2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/20);  total (31/20)

TAAAATTATTTGCTACGGCGACGTACGATGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGGGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTCTG  >  NC_000913/3450538‑3450808                                                                                                                                         |                                                                                                                                       tAAAATTATTTGCTACGGCGACGTACGATGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATgtgagt                                                                                                                                      >  2:1035975/1‑139 (MQ=255) tAAAATTATTTGCTACGGCGACGTACGATGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATgtgagt                                                                                                                                      >  2:171654/1‑139 (MQ=255) tAAAATTATTTGCTACGACGACGTACGATGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTAGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATgtgagt                                                                                                                                      >  2:864392/1‑139 (MQ=255)         tttGCTACGGCGACGTACGATGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGagtggt                                                                                                                                   >  1:148843/1‑134 (MQ=255)         tttGCTACGGCGACGTACGATGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGagtggt                                                                                                                                   <  2:148843/134‑1 (MQ=255)           tGCTACGGCGACGTACGATGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCgg                                                                                                                            >  1:1042117/1‑139 (MQ=255)              tACGGCGACGTACGATGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGtt                                                                                                                         <  1:171654/139‑1 (MQ=255)                     aCGTACGATGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGagtggt                                                                                                                                   >  1:648221/1‑122 (MQ=255)                     aCGTACGATGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGagtggt                                                                                                                                   <  2:648221/122‑1 (MQ=255)                        tACGATGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTa                                                                                                               >  2:909777/1‑139 (MQ=255)                             tGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACcgcg                                                                                                          >  1:1121253/1‑139 (MQ=255)                               aaTTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATgtgagt                                                                                                                                      >  1:1333814/1‑109 (MQ=255)                               aaTTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGaa                                                                                                        >  1:740428/1‑139 (MQ=255)                                         tACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACg                                                                                              >  1:1200451/1‑139 (MQ=255)                                         tACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACg                                                                                              >  1:1066436/1‑139 (MQ=255)                                         tACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACg                                                                                              >  1:727752/1‑139 (MQ=255)                                           cGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGaa                                                                                            >  2:538917/1‑139 (MQ=255)                                                  tgctgcGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACg                                                                                     <  1:771744/139‑1 (MQ=255)                                                     tgcGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCa                                                                                  <  2:1015794/139‑1 (MQ=255)                                                                ttACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCAc                                                                       <  1:909777/139‑1 (MQ=255)                                                                   ccTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATgtgagtg                                                                                                                                     <  1:394477/74‑1 (MQ=255)                                                                   ccTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATgtgagtg                                                                                                                                     >  2:394477/1‑74 (MQ=255)                                                                      ttGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCtt                                                                 >  2:495065/1‑139 (MQ=255)                                                                       tGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCAc                                                                       >  1:416284/1‑132 (MQ=255)                                                                       tGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCAc                                                                       <  2:416284/132‑1 (MQ=255)                                                                       tGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCttt                                                                >  1:898123/1‑139 (MQ=255)                                                                         gTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATgtgagtg                                                                                                                                     <  2:972522/68‑1 (MQ=255)                                                                         gTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATgtgagtg                                                                                                                                     >  1:972522/1‑68 (MQ=255)                                                                         gTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTAc                                                              >  1:626359/1‑139 (MQ=255)                                                                         gTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGCCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTAc                                                              >  2:916918/1‑139 (MQ=255)                                                                            tGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCa                                                           >  2:1378798/1‑139 (MQ=255)                                                                                  ccccACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAAcgc                                                     <  1:1472876/139‑1 (MQ=255)                                                                                   cccACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAAcgcg                                                    >  2:429916/1‑139 (MQ=255)                                                                                       cGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCgcagc                                                <  1:495065/139‑1 (MQ=255)                                                                                         gAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCat                                              <  2:1121253/139‑1 (MQ=255)                                                                                             tACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAAcg                                                      <  1:172365/127‑1 (MQ=255)                                                                                             tACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAAcg                                                      >  2:172365/1‑127 (MQ=255)                                                                                             tACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGc                                          >  1:2755/1‑139 (MQ=255)                                                                                                  ggTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGc                                     >  2:1327902/1‑139 (MQ=255)                                                                                                    tGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCAt                                   >  2:889002/1‑139 (MQ=255)                                                                                                    tGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGATCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCAt                                   >  2:928851/1‑139 (MQ=255)                                                                                                      cTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGa                                                         >  2:269462/1‑115 (MQ=255)                                                                                                      cTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGa                                                         <  1:269462/115‑1 (MQ=255)                                                                                                       ttACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGc                                <  2:1066436/139‑1 (MQ=255)                                                                                                         aCCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCa                              >  2:1411975/1‑139 (MQ=255)                                                                                                             aaGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACtt                          >  2:529415/1‑139 (MQ=255)                                                                                                                 tACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGcc                      <  1:894829/139‑1 (MQ=255)                                                                                                                  aCGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCt                                                                  >  1:1228069/1‑94 (MQ=255)                                                                                                                  aCGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCt                                                                  <  2:1228069/94‑1 (MQ=255)                                                                                                                   cGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCa                    >  2:272575/1‑139 (MQ=255)                                                                                                                         tCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTg              >  1:1389104/1‑139 (MQ=255)                                                                                                                          caccaccacCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGc             >  2:229297/1‑139 (MQ=255)                                                                                                                                caccaTGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCa       >  2:373864/1‑139 (MQ=255)                                                                                                                                 accaTGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAg      >  2:938181/1‑139 (MQ=255)                                                                                                                                 accaTGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAg      <  1:850200/139‑1 (MQ=255)                                                                                                                                  ccaTGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGt     <  1:373864/139‑1 (MQ=255)                                                                                                                                   caTGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTc    <  1:1327902/139‑1 (MQ=255)                                                                                                                                   caTGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTc    <  1:1396084/139‑1 (MQ=255)                                                                                                                                   caTGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTc    <  2:531080/139‑1 (MQ=255)                                                                                                                                   caTGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTc    <  2:1200451/139‑1 (MQ=255)                                                                                                                                    aTGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCa            <  1:1446925/130‑1 (MQ=255)                                                                                                                                    aTGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCa            >  2:1446925/1‑130 (MQ=255)                                                                                                                                     tgtgAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTCTg  >  1:144122/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                       TAAAATTATTTGCTACGGCGACGTACGATGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGGGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTCTG  >  NC_000913/3450538‑3450808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑