Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,485,541	C→T	G93D (GGT→GAT)	yhfA ←	OsmC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,485,541	0	C	T	100.0%	309.8 / NA	88	G93D (GGT→GAT)	yhfA	OsmC family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/46);  total (42/46)

AGTTAAGTCACGCGGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCA  >  NC_000913/3485407‑3485675                                                                                                                                       |                                                                                                                                       aGTTAAGTCACGCGGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGt                                                                                                                                    <  2:249160/139‑1 (MQ=255)   ttAAGTCACGCGGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCTATCGGTGa                                                                                                                                  <  1:981207/139‑1 (MQ=255)   ttAAGTCACGCGGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGa                                                                                                                                  <  2:687705/139‑1 (MQ=255)   ttAAGTCACGCGGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGa                                                                                                                                  <  1:1383900/139‑1 (MQ=255)        tCACGCGGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATa                                                                                                                             >  2:1099946/1‑139 (MQ=255)          aCGCGGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATaaa                                                                                                                           <  2:1231879/139‑1 (MQ=255)            gcgGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAAt                                                                                                                         >  2:1250393/1‑139 (MQ=255)            gcgGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAAt                                                                                                                         <  2:460869/139‑1 (MQ=255)            gcgGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAAt                                                                                                                         <  2:566491/139‑1 (MQ=255)            gcgGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAAt                                                                                                                         <  1:1448078/139‑1 (MQ=255)             cgGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATg                                                                                                                        >  2:219606/1‑139 (MQ=255)             cgGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATg                                                                                                                        <  1:219606/139‑1 (MQ=255)             cgGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATg                                                                                                                        >  2:1413082/1‑139 (MQ=255)                     aCTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTaa                                                                                                                <  2:794591/139‑1 (MQ=255)                     aCTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTaa                                                                                                                <  1:197423/139‑1 (MQ=255)                        tCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAAtgt                                                                                                             >  2:765301/1‑139 (MQ=255)                         cATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGCACAATACTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAAtgtg                                                                                                            >  2:823969/1‑139 (MQ=255)                                   agtAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGa                                                                                                                    <  1:168292/121‑1 (MQ=255)                                   agtAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGa                                                                                                                    >  2:168292/1‑121 (MQ=255)                                   agtAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAgg                                                                                                  >  1:1450083/1‑139 (MQ=255)                                     tAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCg                                                                                                >  1:229321/1‑139 (MQ=255)                                             aCCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTaa                                                                                                                >  1:322220/1‑115 (MQ=255)                                             aCCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTaa                                                                                                                <  2:322220/115‑1 (MQ=255)                                             aCCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCct                                                                                        >  2:272919/1‑139 (MQ=255)                                               cGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCctct                                                                                      >  2:1154792/1‑139 (MQ=255)                                                    tttCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTctcg                                                                                 >  2:349279/1‑139 (MQ=255)                                                    tttCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTcgcg                                                                                 <  1:43697/139‑1 (MQ=255)                                                         aGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGtt                                                                            <  1:272919/139‑1 (MQ=255)                                                           cATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCa                                                                          <  2:359597/139‑1 (MQ=255)                                                           cATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCa                                                                          >  2:1283201/1‑139 (MQ=255)                                                                aCGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGt                                                                     <  2:392175/139‑1 (MQ=255)                                                                aCGCCACTGAACAATACTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGt                                                                     <  1:823969/139‑1 (MQ=255)                                                                         aaCAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTAc                                                            >  2:921531/1‑139 (MQ=255)                                                                              tatTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCgtggtg                                                                                           >  2:1001511/1‑103 (MQ=255)                                                                              tatTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCgtggtg                                                                                           <  1:1001511/103‑1 (MQ=255)                                                                                tttCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACaa                                                     <  2:49642/139‑1 (MQ=255)                                                                                 ttCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTc                                                                    <  1:388944/123‑1 (MQ=255)                                                                                 ttCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTc                                                                    >  2:388944/1‑123 (MQ=255)                                                                                  tctcGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATGAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATc                                                   <  1:1413082/139‑1 (MQ=255)                                                                                   ctcGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATa                                                                                                                             <  2:959583/64‑1 (MQ=255)                                                                                   ctcGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCg                                                  >  1:101803/1‑139 (MQ=255)                                                                                   ctcGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGCGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCg                                                  >  1:296797/1‑139 (MQ=255)                                                                                   ctcGGCAGAGAGATCAACCGCACGCGCAACCGCTGCATCTTTCAGGTAGCGATCGGTGACGAtg                                                                                                                             >  1:959583/1‑63 (MQ=255)                                                                                     cGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTaa                                                                                                                >  1:518611/1‑75 (MQ=255)                                                                                     cGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTaa                                                                                                                >  1:812457/1‑75 (MQ=255)                                                                                     cGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTaa                                                                                                                <  2:812457/75‑1 (MQ=255)                                                                                     cGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTaa                                                                                                                <  2:518611/75‑1 (MQ=255)                                                                                        cAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGt                                                                                                                                    >  1:603457/1‑52 (MQ=255)                                                                                        cAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGt                                                                                                                                    <  2:603457/52‑1 (MQ=255)                                                                                        cAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATa                                                                                                                             <  1:1208545/59‑1 (MQ=255)                                                                                        cAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATa                                                                                                                             >  2:1208545/1‑59 (MQ=255)                                                                                        cAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTcgcg                                                                                 <  1:231892/103‑1 (MQ=255)                                                                                        cAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTcgcg                                                                                 >  2:231892/1‑103 (MQ=255)                                                                                                 cAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACg                                    <  1:1283201/139‑1 (MQ=255)                                                                                                  aaCCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGCGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGc                                   <  2:296797/139‑1 (MQ=255)                                                                                                   aCCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGcc                                  <  1:805705/139‑1 (MQ=255)                                                                                                    ccGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAgg                                                                                                  >  2:650444/1‑74 (MQ=255)                                                                                                    ccGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAgg                                                                                                  <  1:650444/74‑1 (MQ=255)                                                                                                      gCACGCGCGACCGGGGCGGCTTTCAGGTCGCTATCGGTGACGATAAAGTGAAGATTAATGTGCGTAAAAAGGCGTGGTACCTCGTGGCGGCGTTCAGGGGTCAGTTTGACGTCACAAGCGGAAACTTCCTGATGCCCtt                               >  2:1361867/1‑139 (MQ=255)                                                                                                       cACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCaa                                                                  <  2:603130/103‑1 (MQ=255)                                                                                                       cACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCaa                                                                  >  1:603130/1‑103 (MQ=255)                                                                                                       cACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCttt                              >  2:1381198/1‑139 (MQ=255)                                                                                                       cACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCttt                              >  1:114943/1‑139 (MQ=255)                                                                                                       cACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCttt                              >  2:1114524/1‑139 (MQ=255)                                                                                                       cACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCAGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCttt                              >  2:504784/1‑139 (MQ=255)                                                                                                         cgcgCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCg                                                                                                >  2:312731/1‑71 (MQ=255)                                                                                                         cgcgCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCg                                                                                                <  1:312731/71‑1 (MQ=255)                                                                                                         cgcgCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGa                                                 >  2:182933/1‑118 (MQ=255)                                                                                                         cgcgCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGa                                                 <  1:182933/118‑1 (MQ=255)                                                                                                         cgcgCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCttttt                            >  2:654744/1‑139 (MQ=255)                                                                                                          gcgcAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGAGAAAATGCAGATTAATGTGCGTAAACACGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTg                           <  1:916008/139‑1 (MQ=255)                                                                                                            gcAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCag                                                                         <  1:763627/91‑1 (MQ=255)                                                                                                            gcAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCag                                                                         >  2:763627/1‑91 (MQ=255)                                                                                                            gcAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCa                         <  2:229321/139‑1 (MQ=255)                                                                                                            gcAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCa                         <  1:654744/139‑1 (MQ=255)                                                                                                            gcAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCa                         <  1:1114524/139‑1 (MQ=255)                                                                                                            gcAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCa                         <  2:114943/139‑1 (MQ=255)                                                                                                               aCCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGa                      >  2:1263551/1‑139 (MQ=255)                                                                                                                ccGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGAt                     <  1:1263551/139‑1 (MQ=255)                                                                                                                      cGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAAtg                                                                                                              >  1:183055/1‑44 (MQ=255)                                                                                                                      cGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAAtg                                                                                                              <  2:183055/44‑1 (MQ=255)                                                                                                                          tttCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACcaca           >  2:379780/1‑139 (MQ=255)                                                                                                                            tCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCagag                                                                       >  2:698027/1‑77 (MQ=255)                                                                                                                            tCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCagag                                                                       <  1:698027/77‑1 (MQ=255)                                                                                                                               ggTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGAt      <  1:1250393/139‑1 (MQ=255)                                                                                                                                gTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATg     >  1:145647/1‑139 (MQ=255)                                                                                                                                 tCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATgg    >  2:147983/1‑139 (MQ=255)                                                                                                                                   gcgATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCa  <  1:765301/139‑1 (MQ=255)                                                                                                                                   gcgATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCa  >  1:894139/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       AGTTAAGTCACGCGGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCA  >  NC_000913/3485407‑3485675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑