Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,607,418	C→T	A323V (GCC→GTC)	zntA →	zinc, cobalt and lead efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,607,418	0	C	T	100.0%	152.6 / NA	44	A323V (GCC→GTC)	zntA	zinc, cobalt and lead efflux system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/20);  total (24/20)

TCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCCGCGATTATGGCCGTCGCTCTGCTGGTGACGCTGGT  >  NC_000913/3607326‑3607553                                                                                             |                                                                                                                                        tCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGTTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAagag                                                                                           >  2:602321/1‑139 (MQ=255)        gAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCACCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTcgcg                                                                                    <  1:261324/139‑1 (MQ=255)          gcgcgcAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCg                                                                                                                          >  1:181126/1‑99 (MQ=255)          gcgcgcAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCg                                                                                                                          <  2:181126/99‑1 (MQ=255)             cgcAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTccc                                                                               >  1:95422/1‑139 (MQ=255)                    ggCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGc                                                                        <  1:549666/139‑1 (MQ=255)                             gTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCg                                                               <  1:170547/139‑1 (MQ=255)                               cccTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCg                                                                                                                          <  1:1431585/78‑1 (MQ=255)                               cccTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCg                                                                                                                          >  2:1431585/1‑78 (MQ=255)                                   gctgGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGCAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGc                                                                        <  1:998295/124‑1 (MQ=255)                                   gctgGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCAtt                                                                            >  2:467403/1‑120 (MQ=255)                                   gctgGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCAtt                                                                            <  1:467403/120‑1 (MQ=255)                                   gctgGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGc                                                                        >  2:998295/1‑124 (MQ=255)                                       gTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAg                                                     <  1:1026911/139‑1 (MQ=255)                                        tGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTcgcg                                                                                    <  2:495504/107‑1 (MQ=255)                                        tGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTcgcg                                                                                    >  1:495504/1‑107 (MQ=255)                                        gatgtgtataagaGACAGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGc                                                    <  2:920861/127‑1 (MQ=255)                                         gCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGcc                                                   <  1:602321/139‑1 (MQ=255)                                                          tCTGGTGACGTTGGAAGTGCTGTCAGACCCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTcgcg                                                                                    >  2:899042/1‑89 (MQ=255)                                                          tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTcgcg                                                                                    >  2:935745/1‑89 (MQ=255)                                                          tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTcgcg                                                                                    <  1:899042/89‑1 (MQ=255)                                                          tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTcgcg                                                                                    <  1:935745/89‑1 (MQ=255)                                                          tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCcgcg                                  >  1:1494274/1‑139 (MQ=255)                                                          tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCATTTCAGCCGTATCTATACGCCcgcg                                  >  2:917328/1‑139 (MQ=255)                                                           cTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGa                                                                                                                  <  2:607674/58‑1 (MQ=255)                                                           cTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGa                                                                                                                  >  1:607674/1‑58 (MQ=255)                                                           cTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGTGGTTTATCGACCGTTTCAg                                                     <  1:1004516/119‑1 (MQ=255)                                                           cTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAg                                                     >  2:1004516/1‑119 (MQ=255)                                                           cTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCCGCGa                                 >  1:259978/1‑139 (MQ=255)                                                           cTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCCGCGa                                 >  2:216368/1‑139 (MQ=255)                                                            tGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGa                                                                          <  1:334931/97‑1 (MQ=255)                                                            tGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGa                                                                          >  2:334931/1‑97 (MQ=255)                                                             ggTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAaga                                                                                            >  2:269235/1‑78 (MQ=255)                                                             ggTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAaga                                                                                            <  1:269235/78‑1 (MQ=255)                                                                  cGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCCGCGATTATGGc                          >  1:488706/1‑139 (MQ=255)                                                                        aaGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCCGCGATTATGGCCGTCGc                    >  2:772736/1‑139 (MQ=255)                                                                           tGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCCGCGATTATGGCCGTCGctct                 <  1:772736/139‑1 (MQ=255)                                                                             cTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAGCTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGc                                                                        <  2:41340/82‑1 (MQ=255)                                                                             cTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGc                                                                        >  1:41340/1‑82 (MQ=255)                                                                             cTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGt                                                 >  2:698681/1‑105 (MQ=255)                                                                             cTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGt                                                 <  1:698681/105‑1 (MQ=255)                                                                                tCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCCGCGATTATGGCCGTCGCTCTGCTgg            >  2:642854/1‑139 (MQ=255)                                                                                         ggAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCCGCGATTATGGCCGTCGCTCTGCTGGTGACGCTgg   >  1:682120/1‑139 (MQ=255)                                                                                          gAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCCGCGATTATGGCCGTCGCTCTGCTGGTGACGCTGGt  >  1:1314087/1‑139 (MQ=255)                                                                                             |                                                                                                                                        TCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCCGCGATTATGGCCGTCGCTCTGCTGGTGACGCTGGT  >  NC_000913/3607326‑3607553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑