Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,186,292	G→A	A315T (GCG→ACG)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,186,292	0	G	A	100.0%	239.9 / NA	68	A315T (GCG→ACG)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/36);  total (32/36)

CGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAG  >  NC_000913/4186157‑4186421                                                                                                                                        |                                                                                                                                  cgtcgCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGa                                                                                                                                >  1:225419/1‑139 (MQ=255)   tcgCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATc                                                                                                                              >  1:1002675/1‑139 (MQ=255)    cgcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCa                                                                                                                             >  2:1072762/1‑139 (MQ=255)    cgcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCa                                                                                                                             >  1:246644/1‑139 (MQ=255)     gcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                            <  1:1158430/139‑1 (MQ=255)     gcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                            <  1:824368/139‑1 (MQ=255)                   aacaacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATTATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                            >  2:1064402/1‑125 (MQ=255)                   aacaacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                            <  1:1064402/125‑1 (MQ=255)                   aacaacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGc                                                                                                              >  1:549578/1‑139 (MQ=255)                   aacaacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGc                                                                                                              >  2:1475233/1‑139 (MQ=255)                    acaacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCg                                                                                                             >  1:779730/1‑139 (MQ=255)                                     ctgctgGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTgg                                                                                            >  1:1283825/1‑139 (MQ=255)                                      tgctgGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGc                                                                                           >  2:273070/1‑139 (MQ=255)                                       gctgGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGcc                                                                                          >  1:478317/1‑139 (MQ=255)                                       gctgGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGcc                                                                                          <  1:403053/139‑1 (MQ=255)                                          ggATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGAc                                                                                       <  2:1283825/139‑1 (MQ=255)                                           gATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACa                                                                                      <  1:1475233/139‑1 (MQ=255)                                           gATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACa                                                                                      <  2:779730/139‑1 (MQ=255)                                               tGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                  <  1:755841/139‑1 (MQ=255)                                               tGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                  <  2:1042408/139‑1 (MQ=255)                                                ggCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATc                                                                                 <  1:1034368/139‑1 (MQ=255)                                                 gCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCa                                                                                >  1:1149993/1‑139 (MQ=255)                                                  cTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGc                                                                                           >  1:1313263/1‑127 (MQ=255)                                                  cTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGc                                                                                           <  2:1313263/127‑1 (MQ=255)                                                     cgcCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGAc                                                                                       >  2:454183/1‑128 (MQ=255)                                                     cgcCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGAc                                                                                       <  1:454183/128‑1 (MQ=255)                                                       ccGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAAc                                                                                                                  <  1:519097/99‑1 (MQ=255)                                                       ccGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAAc                                                                                                                  >  2:519097/1‑99 (MQ=255)                                                              tcatcGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAggg                                                                   <  2:549578/139‑1 (MQ=255)                                                               catcGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGt                                                                  >  1:1387012/1‑139 (MQ=255)                                                                    tacgtaACGAAAAACGTATGCTGGAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGttt                                                             <  2:8609/139‑1 (MQ=255)                                                                         aaCGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTc                                                        >  2:1103741/1‑139 (MQ=255)                                                                           cGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                  <  1:663282/111‑1 (MQ=255)                                                                           cGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                  >  2:663282/1‑111 (MQ=255)                                                                           cGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAg                                                      >  2:1242539/1‑139 (MQ=255)                                                                            gAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGa                                                     <  2:173986/139‑1 (MQ=255)                                                                               aaaCGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAAcc                                                  <  1:1103741/139‑1 (MQ=255)                                                                                     aTGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTTGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCg                                            >  1:1391509/1‑139 (MQ=255)                                                                                        ctgcAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTa                                         <  1:57094/139‑1 (MQ=255)                                                                                           cAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGAt                                                                                                                               <  2:127219/50‑1 (MQ=255)                                                                                           cAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGAt                                                                                                                               >  1:127219/1‑50 (MQ=255)                                                                                           cAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGc                                      <  2:1149993/139‑1 (MQ=255)                                                                                             ggAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCgt                                    <  2:917731/139‑1 (MQ=255)                                                                                                aGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGtt                                 <  1:273070/139‑1 (MQ=255)                                                                                                   ggTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                            >  1:630197/1‑45 (MQ=255)                                                                                                   ggTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCAc                                                                                                                            <  2:630197/45‑1 (MQ=255)                                                                                                     tAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCt                                              >  2:1276508/1‑121 (MQ=255)                                                                                                     tAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCt                                              <  1:1276508/121‑1 (MQ=255)                                                                                                            ccTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGt                     >  2:855271/1‑139 (MQ=255)                                                                                                            ccTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGt                     >  2:1384882/1‑139 (MQ=255)                                                                                                            ccTGCTGGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGt                     >  1:375742/1‑139 (MQ=255)                                                                                                             ctgctgGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTc                    <  2:375742/139‑1 (MQ=255)                                                                                                               gctgGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGt                  >  1:364762/1‑139 (MQ=255)                                                                                                                ctgGATAACGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGtt                 <  1:1242539/139‑1 (MQ=255)                                                                                                                       aCGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAAt          <  1:791747/139‑1 (MQ=255)                                                                                                                       aCGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAAt          <  2:1387012/139‑1 (MQ=255)                                                                                                                       aCGGTCGTCGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAAt          <  2:225419/139‑1 (MQ=255)                                                                                                                          gtcgtcGCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCAc       >  1:1317700/1‑139 (MQ=255)                                                                                                                             gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGt                                                                           <  1:1168426/68‑1 (MQ=255)                                                                                                                             gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGt                                                                           >  2:1168426/1‑68 (MQ=255)                                                                                                                             gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt    <  1:1072762/139‑1 (MQ=255)                                                                                                                             gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt    <  1:1296419/139‑1 (MQ=255)                                                                                                                             gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt    >  2:287400/1‑139 (MQ=255)                                                                                                                             gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt    <  2:246644/139‑1 (MQ=255)                                                                                                                             gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt    <  1:940572/139‑1 (MQ=255)                                                                                                                             gtcgCGGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt    >  1:966798/1‑139 (MQ=255)                                                                                                                               cgcgGTCGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAg  <  1:61955/139‑1 (MQ=255)                                                                                                                                     cGTACGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                  >  2:1137486/1‑53 (MQ=255)                                                                                                                                        |                                                                                                                                  CGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAG  >  NC_000913/4186157‑4186421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑