Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	337,217	T→C	V147A (GTC→GCC)	yahF →	putative NAD(P)‑binding succinyl‑CoA synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	337,217	0	T	C	100.0%	218.1 / NA	58	V147A (GTC→GCC)	yahF	putative NAD(P)‑binding succinyl‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/32);  total (26/32)

CTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGTCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACAT  >  NC_000913/337085‑337352                                                                                                                                     |                                                                                                                                        cTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGt                                                                                                                                   <  2:1139781/139‑1 (MQ=255) cTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGt                                                                                                                                   <  1:1236034/139‑1 (MQ=255)  tATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGtt                                                                                                                                  <  2:671108/139‑1 (MQ=255)         aGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATg                                                                                                                           >  2:905898/1‑139 (MQ=255)         aGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATg                                                                                                                           <  1:170261/139‑1 (MQ=255)           cgcCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATg                                                                                                                           <  2:545662/137‑1 (MQ=255)           cgcCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATg                                                                                                                           >  1:545662/1‑137 (MQ=255)           cgcCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGaa                                                                                                                         >  1:630968/1‑139 (MQ=255)           cgcCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGaa                                                                                                                         >  2:1432874/1‑139 (MQ=255)           cgcCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGaa                                                                                                                         >  1:943717/1‑139 (MQ=255)           cgcCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGaa                                                                                                                         >  2:1053918/1‑139 (MQ=255)           cgcCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGAGTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGaa                                                                                                                         >  1:375774/1‑139 (MQ=255)            gcCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAAc                                                                                                                        >  2:824058/1‑139 (MQ=255)                                  gTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCa                                                                                                  <  2:630968/139‑1 (MQ=255)                                    aaCCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACg                                                                                                >  2:1077551/1‑139 (MQ=255)                                    aaCCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACg                                                                                                >  2:415692/1‑139 (MQ=255)                                          gCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAACCGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCa                                                                                                               <  1:579312/118‑1 (MQ=255)                                          gCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCa                                                                                                               >  2:579312/1‑118 (MQ=255)                                           cGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCa                                                                                                  <  2:841884/130‑1 (MQ=255)                                           cGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCa                                                                                                  >  1:841884/1‑130 (MQ=255)                                            ggTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAgggg                                                                                        <  1:905898/139‑1 (MQ=255)                                            ggTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAggg                                                                                        <  1:1053918/139‑1 (MQ=255)                                            ggTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAggg                                                                                        <  1:223856/139‑1 (MQ=255)                                                  ttCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGctgctg                                                                                  <  1:1017209/139‑1 (MQ=255)                                                      gTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCtgatga                                                                              <  2:943717/139‑1 (MQ=255)                                                          aCGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACt                                                                                                                       >  2:31099/1‑94 (MQ=255)                                                          aCGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACt                                                                                                                       <  1:31099/94‑1 (MQ=255)                                                           cgcacggtgtGCCGCTCGCGAAGCGAGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATgggg                                                                         <  2:375774/130‑1 (MQ=255)                                                                                aGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGAtt                                                    >  1:1122405/1‑139 (MQ=255)                                                                                              tGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAAcggcgcggc                                      >  1:990320/1‑139 (MQ=255)                                                                                               gCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCa                                                                                                  <  1:1440535/78‑1 (MQ=255)                                                                                               gCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCa                                                                                                  >  2:1440535/1‑78 (MQ=255)                                                                                               gCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAAcggcgcggcg                                     >  1:620097/1‑139 (MQ=255)                                                                                                 aaaaCGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGct                                   <  1:1077551/139‑1 (MQ=255)                                                                                                 aaaaCGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGct                                   <  1:415692/139‑1 (MQ=255)                                                                                                     cGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGt                               <  1:1432874/139‑1 (MQ=255)                                                                                                       aTCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGttt                             <  2:1122405/139‑1 (MQ=255)                                                                                                       aTCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGttt                             <  1:816004/139‑1 (MQ=255)                                                                                                        tctcAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGtttt                            >  1:400907/1‑139 (MQ=255)                                                                                                           cAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGt                         <  1:594242/139‑1 (MQ=255)                                                                                                                tGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGc                    >  2:1169558/1‑139 (MQ=255)                                                                                                                 gATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGa                                                      >  1:230936/1‑104 (MQ=255)                                                                                                                 gATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGa                                                      <  2:230936/104‑1 (MQ=255)                                                                                                                  aTGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACggcgcggcgc                                    <  1:149576/121‑1 (MQ=255)                                                                                                                  aTGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACggcgcggcgc                                    >  2:149576/1‑121 (MQ=255)                                                                                                                     cTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGc               <  2:990320/139‑1 (MQ=255)                                                                                                                      tGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCa                                                                      <  1:1370348/83‑1 (MQ=255)                                                                                                                      tGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCa                                                                      >  2:1370348/1‑83 (MQ=255)                                                                                                                       gTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGt             <  2:620097/139‑1 (MQ=255)                                                                                                                             cGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGc       >  1:931211/1‑139 (MQ=255)                                                                                                                               aTAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCaa     <  1:1329591/139‑1 (MQ=255)                                                                                                                               aTAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCaa     >  2:1329591/1‑139 (MQ=255)                                                                                                                                tAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGc                                                                        <  2:654090/71‑1 (MQ=255)                                                                                                                                tAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGc                                                                        >  1:654090/1‑71 (MQ=255)                                                                                                                                tAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACggcgcggcgc                                    <  2:754534/107‑1 (MQ=255)                                                                                                                                tAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACggcgcggcgc                                    >  1:754534/1‑107 (MQ=255)                                                                                                                                  aCGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACAt  <  2:400907/139‑1 (MQ=255)                                                                                                                                  aCGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACAt  <  1:1169558/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        CTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGTCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACAT  >  NC_000913/337085‑337352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑