Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,361,357 A→G L193S (TTG→TCG)  cadC ← cadBA operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,361,3570AG100.0% 281.6 / NA 74L193S (TTG→TCG) cadCcadBA operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/34);  total (40/34)

AAGGTGGAGAAGGTGTTAAGTGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGA  >  NC_000913/4361225‑4361490
                                                                                                                                    |                                                                                                                                     
aaGGTGGAGAAGGTGTTAAGTGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATAc                                                                                                                                 <  2:1154111/139‑1 (MQ=255)
    tGGAGAAGGTGTTAAGTGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGa                                                                                                                             <  1:170465/139‑1 (MQ=255)
      gagaAGGTGTTAAGTGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCgaaa                                                                                                                                    <  1:818715/130‑1 (MQ=255)
      gagaAGGTGTTAAGTGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCgaaa                                                                                                                                    >  2:818715/1‑130 (MQ=255)
      gagaAGGTGTTAAGTGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGAtt                                                                                                                           >  2:55180/1‑139 (MQ=255)
      gagaAGGTGTTAAGTGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGAtt                                                                                                                           >  1:1383827/1‑139 (MQ=255)
      gagaAGGTGTTAAGTGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGAtt                                                                                                                           >  2:420844/1‑139 (MQ=255)
         aaGGTGTTAAGTGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGc                                                                                                                        <  2:460865/139‑1 (MQ=255)
          aGGTGTTAAGTGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCt                                                                                                                       <  2:1383827/139‑1 (MQ=255)
           ggTGTTAAGTGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTc                                                                                                                      >  1:795594/1‑139 (MQ=255)
             tgtTAAGTGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCAt                                                                                                                    >  2:530731/1‑139 (MQ=255)
                tAAGTGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAgg                                                                                                                 >  2:1074924/1‑139 (MQ=255)
                       tGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGAtt                                                                                                                           >  2:467277/1‑122 (MQ=255)
                       tGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGAtt                                                                                                                           <  1:467277/122‑1 (MQ=255)
                       tGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTg                                                                                                                         <  2:1147871/124‑1 (MQ=255)
                       tGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTg                                                                                                                         >  1:1147871/1‑124 (MQ=255)
                       tGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACgt                                                                                                          >  2:676058/1‑139 (MQ=255)
                       tGTAGCCACCAAATCACACACGCCTATCGCGTAAGCGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTGTTTACCATATTAATGTCACTACCGCGTGGATTCAGAGACATACGTGATTTGCTCATAGGAACACgt                                                                                                          >  2:232357/1‑139 (MQ=255)
                         tCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGaa                                                                                                               >  2:1465347/1‑132 (MQ=255)
                         tCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGaa                                                                                                               <  1:1465347/132‑1 (MQ=255)
                         tCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACgtgt                                                                                                        >  1:1203757/1‑139 (MQ=255)
                            ccaccaAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATc                                                                                                     <  1:788970/139‑1 (MQ=255)
                                   aTCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACtt                                                                                              >  2:1176353/1‑139 (MQ=255)
                                              cTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTAc                                                                                   <  1:55180/139‑1 (MQ=255)
                                              cTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTAc                                                                                   >  1:1047946/1‑139 (MQ=255)
                                              cTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTAc                                                                                   <  2:1203757/139‑1 (MQ=255)
                                              cTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTAc                                                                                   <  1:1074924/139‑1 (MQ=255)
                                                aTCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCa                                                                                 <  1:256303/139‑1 (MQ=255)
                                                         gagagCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTacac                                                                        >  1:877672/1‑139 (MQ=255)
                                                                      gggAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGa                                                           <  2:800149/139‑1 (MQ=255)
                                                                       ggAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGATATACGCGATTTGCt                                                                                                                       <  1:915217/78‑1 (MQ=255)
                                                                       ggAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCt                                                                                                                       >  2:915217/1‑78 (MQ=255)
                                                                       ggAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGac                                                          >  1:1273206/1‑139 (MQ=255)
                                                                           cTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTAcaca                                                                       >  2:372661/1‑122 (MQ=255)
                                                                           cTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTAcaca                                                                       <  1:372661/122‑1 (MQ=255)
                                                                            tCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAg                                                     <  1:420844/139‑1 (MQ=255)
                                                                              cAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGa                                                   <  1:1176353/139‑1 (MQ=255)
                                                                                     tACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGaaaaaaaa                                            <  2:451488/139‑1 (MQ=255)
                                                                                         aCTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCAt                                        <  1:450726/139‑1 (MQ=255)
                                                                                                    ccATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTAc                                                                                   <  2:429735/85‑1 (MQ=255)
                                                                                                    ccATATTAATGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTAc                                                                                   >  1:429735/1‑85 (MQ=255)
                                                                                                             tGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTg                                                                                                                         >  1:536100/1‑38 (MQ=255)
                                                                                                             tGTCAATATCGCGTGGATTGAGCGAAATACGCGATTTg                                                                                                                         <  2:536100/38‑1 (MQ=255)
                                                                                                               tCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTaca                                                                         <  2:1141793/84‑1 (MQ=255)
                                                                                                               tCAATATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTaca                                                                         >  1:1141793/1‑84 (MQ=255)
                                                                                                                 aaTATCGCGTGGATTGAGCGAAATACGCTATTTGTTCATAGGAAGACGTATATCAAGACTTAAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTg                >  2:975907/1‑139 (MQ=255)
                                                                                                                 aaTATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAgg                                                                                                                 <  1:1079013/42‑1 (MQ=255)
                                                                                                                 aaTATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAgg                                                                                                                 >  2:1079013/1‑42 (MQ=255)
                                                                                                                 aaTATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGa                                                                                                                <  2:520107/43‑1 (MQ=255)
                                                                                                                 aaTATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGa                                                                                                                >  1:520107/1‑43 (MQ=255)
                                                                                                                 aaTATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTg                                                                              >  1:1457703/1‑77 (MQ=255)
                                                                                                                 aaTATCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTg                                                                              <  2:1457703/77‑1 (MQ=255)
                                                                                                                  atatCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTAc                                                                                   <  2:661789/71‑1 (MQ=255)
                                                                                                                  atatCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTAc                                                                                   >  1:661789/1‑71 (MQ=255)
                                                                                                                  atatCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGc               >  1:267056/1‑139 (MQ=255)
                                                                                                                   tatCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGt                   <  2:936263/134‑1 (MQ=255)
                                                                                                                   tatCGCGTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCt              >  1:272378/1‑139 (MQ=255)
                                                                                                                   tatCGCGTGGATTGAGCGAAATACGCGAATTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGt                   >  1:936263/1‑134 (MQ=255)
                                                                                                                      cgcgTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCtttt           >  1:1315989/1‑139 (MQ=255)
                                                                                                                       gcgTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTa          >  1:1360303/1‑139 (MQ=255)
                                                                                                                        cgTGGATTGAGCGAAATACGCGATTTGCTCATAgg                                                                                                                 <  1:1257269/35‑1 (MQ=255)
                                                                                                                        cgTGGATTGAGCGAAATACGCGATTTGCTCATAgg                                                                                                                 >  2:1257269/1‑35 (MQ=255)
                                                                                                                        cgTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCaa                                                                                                   <  2:1107692/49‑1 (MQ=255)
                                                                                                                        cgTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCaa                                                                                                   >  1:1107692/1‑49 (MQ=255)
                                                                                                                        cgTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGaaa                                                                                          >  1:545290/1‑58 (MQ=255)
                                                                                                                        cgTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGaaa                                                                                          <  2:545290/58‑1 (MQ=255)
                                                                                                                        cgTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATAc                                      <  1:1295660/110‑1 (MQ=255)
                                                                                                                        cgTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATAc                                      >  2:1295660/1‑110 (MQ=255)
                                                                                                                        cgTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTaa         >  1:596050/1‑139 (MQ=255)
                                                                                                                        cgTGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTaa         >  1:527445/1‑139 (MQ=255)
                                                                                                                          tGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTAc                                                                                   >  2:1426210/1‑63 (MQ=255)
                                                                                                                          tGGATTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTAc                                                                                   <  1:1426210/63‑1 (MQ=255)
                                                                                                                             aTTGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTggg    >  1:762261/1‑139 (MQ=255)
                                                                                                                               tGAGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGa  >  2:631422/1‑139 (MQ=255)
                                                                                                                                 aGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCt                                                                                     <  1:326513/54‑1 (MQ=255)
                                                                                                                                 aGCGAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCa                                   <  2:801207/104‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                     
AAGGTGGAGAAGGTGTTAAGTGATGTCGCCACCAAATCACCCACGCCTATCGCGTAAGAGAGCTGATACGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGA  >  NC_000913/4361225‑4361490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: