Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 367,852 (GC)6→5 coding (568‑569/834 nt) mhpR ← mhp operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913367,8420G.100.0% 150.2 / NA 36coding (579/834 nt)mhpRmhp operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (19/17);  total (19/17)
*NC_000913367,8430C.100.0% 150.2 / NA 36coding (578/834 nt)mhpRmhp operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (19/17);  total (19/17)

TTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGT  >  NC_000913/367704‑367970
                                                                                                                                          ||                                                                                                                               
ttCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTg                                                                                                                                  >  1:64886/1‑139 (MQ=255)
                             ccAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTa                                                                                                   >  1:369608/1‑139 (MQ=255)
                                 aTTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACgg                                                                                               >  1:1060382/1‑139 (MQ=255)
                                 aTTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACgg                                                                                               >  1:723870/1‑139 (MQ=255)
                                 aTTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCGGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACgg                                                                                               >  1:700583/1‑139 (MQ=255)
                                    cAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTc                                                                                            <  2:723870/139‑1 (MQ=255)
                                     aGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTcc                                                                                           >  2:878920/1‑139 (MQ=255)
                                      gACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAGCCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTccc                                                                                          <  1:273852/139‑1 (MQ=255)
                                           ccAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATTCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                                                                     <  2:700583/139‑1 (MQ=255)
                                            cAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCa                                                                                    >  2:586376/1‑139 (MQ=255)
                                              aTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGt                                                                                  <  2:1060382/139‑1 (MQ=255)
                                                         ttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTGAACGGTTCCCGTGCCAGTTGATAGtcatc                                                                       >  2:713346/1‑139 (MQ=255)
                                                            aCTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCAcc                                                                    <  2:728735/139‑1 (MQ=255)
                                                             cTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCg                                                                   >  1:28882/1‑139 (MQ=255)
                                                                     cGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGa                                                           >  2:519200/1‑139 (MQ=255)
                                                                               aTAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCg                                                              <  1:122651/126‑1 (MQ=255)
                                                                               aTAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCg                                                              >  2:122651/1‑126 (MQ=255)
                                                                                     gCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGt                                                                                  <  2:905960/100‑1 (MQ=255)
                                                                                     gCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGt                                                                                  >  1:905960/1‑100 (MQ=255)
                                                                                      cGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTa                                          >  2:29509/1‑139 (MQ=255)
                                                                                              tcctccTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTg                                  <  2:28882/139‑1 (MQ=255)
                                                                                              tcctccTGATCCCAGCCGCGGTAGTACTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTg                                  <  1:713346/139‑1 (MQ=255)
                                                                                                   ctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GTGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTc                             >  1:355389/1‑139 (MQ=255)
                                                                                                   ctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTc                             >  1:827760/1‑139 (MQ=255)
                                                                                                   ctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTc                             >  1:403613/1‑139 (MQ=255)
                                                                                                             ccGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTCACGGTTCCCGTGCCAGATGa                                                                               <  1:749997/79‑1 (MQ=255)
                                                                                                             ccGCGGTAGTTCTGTCCGTAACCCCCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGa                                                                               >  2:749997/1‑79 (MQ=255)
                                                                                                                         tGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagc       <  1:878920/139‑1 (MQ=255)
                                                                                                                         tGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagc       <  2:827760/139‑1 (MQ=255)
                                                                                                                             cGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCgg                                                             <  2:1068061/81‑1 (MQ=255)
                                                                                                                             cGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCgg                                                             >  1:1068061/1‑81 (MQ=255)
                                                                                                                              gTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGt  <  2:403613/139‑1 (MQ=255)
                                                                                                                              gTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGt  <  1:1014835/139‑1 (MQ=255)
                                                                                                                                aaCCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg                                                                                       >  2:217911/1‑52 (MQ=255)
                                                                                                                                aaCCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg                                                                                       <  1:217911/52‑1 (MQ=255)
                                                                                                                                aaCCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGAt                                            >  1:182573/1‑95 (MQ=255)
                                                                                                                                aaCCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGAt                                            <  2:182573/95‑1 (MQ=255)
                                                                                                                                          ||                                                                                                                               
TTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGT  >  NC_000913/367704‑367970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: