| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,763,869 | (T)7→8 | coding (1285/1617 nt) | yfjL ← | CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,763,862 | 1 | . | T | 98.2% | 183.9 / ‑3.5 | 54 | coding (1292/1617 nt) | yfjL | CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (29/24); total (30/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
TAGATTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑TTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGG > NC_000913/2763732‑2763995 | tagATTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑ttt‑ttttt < 1:973401/139‑2 (MQ=255) aTTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑ttt‑tttttgcg < 2:904848/139‑5 (MQ=255) tAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTgcgcg < 2:1048055/139‑1 (MQ=255) tAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTgcgcg > 1:539517/1‑139 (MQ=255) agatTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGCGGTTAGCATCGAAAGGTTTGCTGCTATCAAGAACTTCGTCAATGTCTTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTgcgcgc < 2:807945/139‑1 (MQ=255) agTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCgg > 1:880594/1‑139 (MQ=255) tCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTa > 1:299701/1‑139 (MQ=255) tCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTa > 1:50132/1‑139 (MQ=255) tAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCg > 1:918762/1‑139 (MQ=255) cACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCa > 1:630676/1‑139 (MQ=255) cGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAAt > 1:798943/1‑139 (MQ=255) caAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGt < 1:853165/139‑1 (MQ=255) aaaCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTc < 2:50132/139‑1 (MQ=255) aaCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc < 2:798943/139‑1 (MQ=255) aCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCt > 1:745366/1‑139 (MQ=255) aaaCGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTgcgcgc < 1:824277/108‑1 (MQ=255) aaaCGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTgcgcgc > 2:824277/1‑108 (MQ=255) aaaCGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc > 2:33582/1‑133 (MQ=255) aaaCGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc < 1:33582/133‑1 (MQ=255) aaaCGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTAc > 1:483373/1‑139 (MQ=255) gttcaaGGTGAGCATCGAAATGTTTGCTTCCATCAAGACTTTCGTCACTCTCGTGGCGAACCAGGTAGTTATCGTCTTTAATGACGACAATTTT‑TTTTGCGCGTCGTACCGACGCACTCCCAAATGTCCTCGTACAACt < 2:686835/134‑1 (MQ=255) gATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACt > 2:527188/1‑139 (MQ=255) aTCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc > 2:1006425/1‑128 (MQ=255) aTCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc < 1:1006425/128‑1 (MQ=255) cGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGAt > 1:1105466/1‑139 (MQ=255) aGCATCGAAAGGTTGACTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACt < 2:588198/129‑1 (MQ=255) aGCATCGAAAGGTTGACTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑TTTATTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACt > 1:588198/1‑129 (MQ=255) cATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTgcgcg < 1:747127/91‑1 (MQ=255) cATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTgcgcg > 2:747127/1‑91 (MQ=255) gCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGAt < 1:247706/88‑1 (MQ=255) gCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGAt > 2:247706/1‑88 (MQ=255) gCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGc < 1:228096/139‑1 (MQ=255) gCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGc < 2:1105466/139‑1 (MQ=255) gCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGc < 2:73302/139‑1 (MQ=255) ccATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 2:918762/139‑1 (MQ=255) ccATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 2:299701/139‑1 (MQ=255) cGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGc > 2:618779/1‑73 (MQ=255) cGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGc < 1:618779/73‑1 (MQ=255) aTGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCAcc > 1:339126/1‑139 (MQ=255) tCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAAc < 2:745366/139‑1 (MQ=255) aaGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGaa > 1:629064/1‑139 (MQ=255) aGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAg > 1:984698/1‑139 (MQ=255) gCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGc > 2:779815/1‑107 (MQ=255) gCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGc < 1:779815/107‑1 (MQ=255) gCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGc > 1:533434/1‑139 (MQ=255) gCAGGTAGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGc < 2:984698/139‑1 (MQ=255) aGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa > 2:205229/1‑102 (MQ=255) aGTTATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 1:205229/102‑1 (MQ=255) tATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCaa > 1:249769/1‑138 (MQ=255) tATCGTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCaa < 2:249769/138‑1 (MQ=255) gTCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTg > 2:146126/1‑139 (MQ=255) tCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGa > 1:259466/1‑139 (MQ=255) tCTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGa > 2:99652/1‑139 (MQ=255) cTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGAt < 2:630676/139‑1 (MQ=255) cTTTAATGTCGAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGAt > 2:853919/1‑139 (MQ=255) gAGGATTTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCACGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCGAAGCTGATCACCTTCTgg > 2:783475/1‑139 (MQ=255) | TAGATTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑TTT‑TTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGG > NC_000913/2763732‑2763995 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |