Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,481,041	A→G	T45A (ACG→GCG)	ynbD →	putative phosphatase inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,481,041	0	A	G	100.0%	203.3 / NA	54	T45A (ACG→GCG)	ynbD	putative phosphatase inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/25);  total (29/25)

TGCTACAAGGCGCTGGCTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTA  >  NC_000913/1480910‑1481175                                                                                                                                    |                                                                                                                                       tGCTACAAGGCGCTGGCTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGAt                                                                                                                                 <  2:382562/139‑1 (MQ=255)         ggCGCTGGCTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTttc                                                                                                                         >  1:258199/1‑139 (MQ=255)                                ggCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTAc                                                                                                  >  1:1034747/1‑139 (MQ=255)                                         tttCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTg                                                                                         <  2:1034747/139‑1 (MQ=255)                                                         ggATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGAt                                                                         >  2:1057282/1‑139 (MQ=255)                                                          gATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTcc                                                                                                                     >  2:961186/1‑94 (MQ=255)                                                          gATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTcc                                                                                                                     <  1:961186/94‑1 (MQ=255)                                                               ctTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTcc                                                                                                                     <  2:902961/89‑1 (MQ=255)                                                               ctTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTcc                                                                                                                     >  1:902961/1‑89 (MQ=255)                                                               ctTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGc                                                                   >  1:258117/1‑139 (MQ=255)                                                                ttAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTcc                                                                                                      <  2:860127/103‑1 (MQ=255)                                                                ttAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTcc                                                                                                      >  1:860127/1‑103 (MQ=255)                                                                  aaTCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGt                                                                <  2:991368/139‑1 (MQ=255)                                                                        ttCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGa                                                                                                                >  1:798591/1‑85 (MQ=255)                                                                        ttCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGa                                                                                                                <  2:798591/85‑1 (MQ=255)                                                                        ttCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGttt                                                          >  1:612556/1‑139 (MQ=255)                                                                         tCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTg                                                         >  1:674481/1‑139 (MQ=255)                                                                          cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGAt                                                                         <  1:899514/122‑1 (MQ=255)                                                                          cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGAt                                                                         >  2:899514/1‑122 (MQ=255)                                                                          cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGt                                                        >  2:26410/1‑139 (MQ=255)                                                                          cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGt                                                        >  2:469257/1‑139 (MQ=255)                                                                               cgGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTTTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCAc                                                   <  2:612556/139‑1 (MQ=255)                                                                                  ttCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTg                                                                 >  1:762073/1‑122 (MQ=255)                                                                                  ttCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTg                                                                 <  2:762073/122‑1 (MQ=255)                                                                                        agcTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCGTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGa                                          <  1:721501/137‑1 (MQ=255)                                                                                            tAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATcc                                                                                                                               >  1:526069/1‑50 (MQ=255)                                                                                            tAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATcc                                                                                                                               <  2:526069/50‑1 (MQ=255)                                                                                            tAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTgg                                                                                        >  1:1078523/1‑89 (MQ=255)                                                                                            tAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTgg                                                                                        <  2:1078523/89‑1 (MQ=255)                                                                                            tAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAg                                      >  1:588678/1‑139 (MQ=255)                                                                                            tAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAg                                      >  2:963469/1‑139 (MQ=255)                                                                                              aCAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAgcg                                    <  2:258199/139‑1 (MQ=255)                                                                                                aGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGcc                                  <  1:1057282/139‑1 (MQ=255)                                                                                                  ccATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTc                                                                                           <  2:1099259/80‑1 (MQ=255)                                                                                                  ccATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTc                                                                                           >  1:1099259/1‑80 (MQ=255)                                                                                                    aTGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCtttt                                                                                                                          >  2:455830/1‑47 (MQ=255)                                                                                                    aTGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCtttt                                                                                                                          <  1:455830/47‑1 (MQ=255)                                                                                                    aTGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGtt                                                                                                        <  2:298323/65‑1 (MQ=255)                                                                                                    aTGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGtt                                                                                                        >  1:298323/1‑65 (MQ=255)                                                                                                    aTGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACt                              >  1:413204/1‑139 (MQ=255)                                                                                                     tGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGCGATTCGAACAGCGCCGAcct                             >  2:721501/1‑137 (MQ=255)                                                                                                       atatCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCtt                                                                                   <  1:807180/83‑1 (MQ=255)                                                                                                       atatCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCtt                                                                                   >  2:807180/1‑83 (MQ=255)                                                                                                             ccAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCg                     >  1:907288/1‑139 (MQ=255)                                                                                                                gTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTg                                                                                                          >  1:982558/1‑51 (MQ=255)                                                                                                                gTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTg                                                                                                          <  2:982558/51‑1 (MQ=255)                                                                                                                gTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACAt                                              >  1:1104267/1‑111 (MQ=255)                                                                                                                gTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGAACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACAt                                              <  2:1104267/111‑1 (MQ=255)                                                                                                                 tCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCtt                 >  1:66929/1‑139 (MQ=255)                                                                                                                    ggTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTAtt              <  2:907288/139‑1 (MQ=255)                                                                                                                        ttCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGCTTTTCGCTGTTCGTTTGTAGCACGACTTTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTgg          >  2:442813/1‑139 (MQ=255)                                                                                                                           ggTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCaa       <  1:963469/139‑1 (MQ=255)                                                                                                                           ggTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCaa       <  1:26410/139‑1 (MQ=255)                                                                                                                                ggAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTa  <  2:674481/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       TGCTACAAGGCGCTGGCTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTA  >  NC_000913/1480910‑1481175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑