Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,572,786 | A→G | V806A (GTT→GCT) | pqqL ← | putative periplasmic M16 family zinc metalloendopeptidase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,572,786 | 0 | A | G | 100.0% | 208.0 / NA | 55 | V806A (GTT→GCT) | pqqL | putative periplasmic M16 family zinc metalloendopeptidase |
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/33); total (22/33) |
GATCCCTTTAGCCAGACGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAACGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCAC > NC_000913/1572650‑1572914 | gATCCCTTTAGCCAGACGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCg < 1:201376/139‑1 (MQ=255) gATCCCTTTAGCCAGACGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCg < 1:481979/139‑1 (MQ=255) aTCCCTTTAGCCAGACGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCga > 2:384233/1‑138 (MQ=255) ttAGCCAGACGCTTAACCATCACTTCAATCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg < 1:1084639/139‑1 (MQ=255) aGCCAGACGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCt > 1:333484/1‑139 (MQ=255) aCGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGAt > 1:454980/1‑139 (MQ=255) aCGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGAt > 1:947823/1‑139 (MQ=255) aCGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGAt > 1:646257/1‑139 (MQ=255) gCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGc < 2:599948/139‑1 (MQ=255) cATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCa < 2:811387/139‑1 (MQ=255) aTCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCAc < 1:515312/139‑1 (MQ=255) aTCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCAc < 1:458027/139‑1 (MQ=255) tCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGc > 2:292051/1‑130 (MQ=255) tCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGc < 1:292051/130‑1 (MQ=255) tCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACg < 2:646257/139‑1 (MQ=255) tCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACg < 1:984182/139‑1 (MQ=255) cACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGa < 2:355388/139‑1 (MQ=255) aTTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTa < 2:947823/139‑1 (MQ=255) cTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATAATAACACg > 2:249402/1‑139 (MQ=255) aaCGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTa > 1:760927/1‑139 (MQ=255) aaCGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTa < 1:249402/139‑1 (MQ=255) aaCGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTa < 1:169694/139‑1 (MQ=255) aCAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg < 1:254273/99‑1 (MQ=255) aCAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg > 2:254273/1‑99 (MQ=255) tcatcaTGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGACGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTTCCa > 1:627594/1‑139 (MQ=255) catcatGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg > 1:287354/1‑93 (MQ=255) catcatGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg < 2:287354/93‑1 (MQ=255) catcatGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCAAg > 1:275552/1‑139 (MQ=255) tcatGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTg < 2:333484/139‑1 (MQ=255) catGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGc < 2:275552/139‑1 (MQ=255) atGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCg < 1:250867/139‑1 (MQ=255) atGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCg < 1:11619/139‑1 (MQ=255) gTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGAc < 2:454980/139‑1 (MQ=255) ttCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCt < 2:397278/78‑1 (MQ=255) ttCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCt > 1:397278/1‑78 (MQ=255) ttCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCt < 2:266731/78‑1 (MQ=255) ttCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCt > 1:266731/1‑78 (MQ=255) gACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg > 2:200710/1‑75 (MQ=255) gACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg < 1:200710/75‑1 (MQ=255) aCAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCg < 2:760927/139‑1 (MQ=255) aGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCAGCGAGCGCCATa > 1:717290/1‑139 (MQ=255) gCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGc > 2:975672/1‑70 (MQ=255) gCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGc < 1:975672/70‑1 (MQ=255) cAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCa > 2:1025544/1‑131 (MQ=255) cAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCa < 1:1025544/131‑1 (MQ=255) aTGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGc < 2:717290/139‑1 (MQ=255) atatCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCg < 1:264107/42‑1 (MQ=255) aGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCgg > 2:468370/1‑117 (MQ=255) aGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCgg < 1:468370/117‑1 (MQ=255) aaCCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATaa > 2:1086659/1‑139 (MQ=255) cGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGc < 1:948534/139‑1 (MQ=255) gagGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATAtt < 1:935473/48‑1 (MQ=255) gagGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATAtt > 2:935473/1‑48 (MQ=255) agtcGAGAAGAAGTGCTGTATGCGCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAATAGAATCGAGCGCCATACGCCTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTcc < 2:730155/136‑1 (MQ=255) gCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCAc > 1:1012459/1‑139 (MQ=255) | GATCCCTTTAGCCAGACGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAACGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCAC > NC_000913/1572650‑1572914 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |