Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,572,786 A→G V806A (GTT→GCT)  pqqL ← putative periplasmic M16 family zinc metalloendopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,572,7860AG100.0% 208.0 / NA 55V806A (GTT→GCT) pqqLputative periplasmic M16 family zinc metalloendopeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/33);  total (22/33)

GATCCCTTTAGCCAGACGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAACGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCAC  >  NC_000913/1572650‑1572914
                                                                                                                                        |                                                                                                                                
gATCCCTTTAGCCAGACGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCg                                                                                                                                <  1:201376/139‑1 (MQ=255)
gATCCCTTTAGCCAGACGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCg                                                                                                                                <  1:481979/139‑1 (MQ=255)
 aTCCCTTTAGCCAGACGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCga                                                                                                                               >  2:384233/1‑138 (MQ=255)
       ttAGCCAGACGCTTAACCATCACTTCAATCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg                                                                                                                         <  1:1084639/139‑1 (MQ=255)
         aGCCAGACGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCt                                                                                                                       >  1:333484/1‑139 (MQ=255)
               aCGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGAt                                                                                                                 >  1:454980/1‑139 (MQ=255)
               aCGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGAt                                                                                                                 >  1:947823/1‑139 (MQ=255)
               aCGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGAt                                                                                                                 >  1:646257/1‑139 (MQ=255)
                 gCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGc                                                                                                               <  2:599948/139‑1 (MQ=255)
                        cATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCa                                                                                                        <  2:811387/139‑1 (MQ=255)
                         aTCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCAc                                                                                                       <  1:515312/139‑1 (MQ=255)
                         aTCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCAc                                                                                                       <  1:458027/139‑1 (MQ=255)
                          tCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGc                                                                                                               >  2:292051/1‑130 (MQ=255)
                          tCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGc                                                                                                               <  1:292051/130‑1 (MQ=255)
                          tCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACg                                                                                                      <  2:646257/139‑1 (MQ=255)
                          tCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACg                                                                                                      <  1:984182/139‑1 (MQ=255)
                           cACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGa                                                                                                     <  2:355388/139‑1 (MQ=255)
                                 aTTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTa                                                                                               <  2:947823/139‑1 (MQ=255)
                                      cTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATAATAACACg                                                                                          >  2:249402/1‑139 (MQ=255)
                                        aaCGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTa                                                                                        >  1:760927/1‑139 (MQ=255)
                                        aaCGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTa                                                                                        <  1:249402/139‑1 (MQ=255)
                                        aaCGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTa                                                                                        <  1:169694/139‑1 (MQ=255)
                                               aCAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg                                                                                                                         <  1:254273/99‑1 (MQ=255)
                                               aCAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg                                                                                                                         >  2:254273/1‑99 (MQ=255)
                                                    tcatcaTGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGACGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTTCCa                                                                            >  1:627594/1‑139 (MQ=255)
                                                     catcatGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg                                                                                                                         >  1:287354/1‑93 (MQ=255)
                                                     catcatGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg                                                                                                                         <  2:287354/93‑1 (MQ=255)
                                                     catcatGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCAAg                                                                           >  1:275552/1‑139 (MQ=255)
                                                       tcatGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTg                                                                         <  2:333484/139‑1 (MQ=255)
                                                        catGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGc                                                                        <  2:275552/139‑1 (MQ=255)
                                                         atGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCg                                                                       <  1:250867/139‑1 (MQ=255)
                                                         atGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCg                                                                       <  1:11619/139‑1 (MQ=255)
                                                           gTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGAc                                                                     <  2:454980/139‑1 (MQ=255)
                                                               ttCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCt                                                                                                                              <  2:397278/78‑1 (MQ=255)
                                                               ttCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCt                                                                                                                              >  1:397278/1‑78 (MQ=255)
                                                               ttCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCt                                                                                                                              <  2:266731/78‑1 (MQ=255)
                                                               ttCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCt                                                                                                                              >  1:266731/1‑78 (MQ=255)
                                                                       gACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg                                                                                                                         >  2:200710/1‑75 (MQ=255)
                                                                       gACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg                                                                                                                         <  1:200710/75‑1 (MQ=255)
                                                                        aCAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCg                                                        <  2:760927/139‑1 (MQ=255)
                                                                                 aGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCAGCGAGCGCCATa                                               >  1:717290/1‑139 (MQ=255)
                                                                                      gCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGc                                                                                                               >  2:975672/1‑70 (MQ=255)
                                                                                      gCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGc                                                                                                               <  1:975672/70‑1 (MQ=255)
                                                                                       cAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCa                                                 >  2:1025544/1‑131 (MQ=255)
                                                                                       cAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCa                                                 <  1:1025544/131‑1 (MQ=255)
                                                                                          aTGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGc                                      <  2:717290/139‑1 (MQ=255)
                                                                                                 atatCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCg                                                                                                                                <  1:264107/42‑1 (MQ=255)
                                                                                                               aGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCgg                                       >  2:468370/1‑117 (MQ=255)
                                                                                                               aGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCgg                                       <  1:468370/117‑1 (MQ=255)
                                                                                                                     aaCCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATaa           >  2:1086659/1‑139 (MQ=255)
                                                                                                                        cGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGc        <  1:948534/139‑1 (MQ=255)
                                                                                                                           gagGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATAtt                                                                                                <  1:935473/48‑1 (MQ=255)
                                                                                                                           gagGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATAtt                                                                                                >  2:935473/1‑48 (MQ=255)
                                                                                                                            agtcGAGAAGAAGTGCTGTATGCGCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAATAGAATCGAGCGCCATACGCCTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTcc    <  2:730155/136‑1 (MQ=255)
                                                                                                                              gCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCAc  >  1:1012459/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                
GATCCCTTTAGCCAGACGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAACGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCAC  >  NC_000913/1572650‑1572914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: