Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,629,913	G→A	P334L (CCG→CTG)	ydfI ←	putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,629,913	0	G	A	100.0%	220.5 / NA	63	P334L (CCG→CTG)	ydfI	putative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/36);  total (27/36)

CCGCTGTGGCAATTTCTGGCTACCATCCATCGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAATGAATGACT  >  NC_000913/1629783‑1630031                                                                                                                                   |                                                                                                                       ccGCTGTGGCAATTTCTGGCTACCATCCATCGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGt                                                                                                                <  1:40224/139‑1 (MQ=255)      gtgGCAATTTCTGGCTACCATCCATCGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTg                                                                                                           <  1:1048902/139‑1 (MQ=255)                cTGGCTACCATCCATCGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAg                                                                                                 <  1:582703/139‑1 (MQ=255)                   gCTACCATCCATCGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACa                                                                                                      <  2:412597/131‑1 (MQ=255)                   gCTACCATCCATCGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACa                                                                                                      >  1:412597/1‑131 (MQ=255)                   gCTACCATCCATCGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCa                                                                                              >  2:283953/1‑139 (MQ=255)                          tccatcGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATAcgccg                                                                                       <  2:108944/139‑1 (MQ=255)                               cGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCa                                                                                     <  1:847969/136‑1 (MQ=255)                               cGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCa                                                                                     >  2:847969/1‑136 (MQ=255)                                  aaTCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATa                                                                                            >  1:390259/1‑126 (MQ=255)                                  aaTCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATa                                                                                            <  2:390259/126‑1 (MQ=255)                                          aGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTc                                                                       <  2:910512/139‑1 (MQ=255)                                          aGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTc                                                                       <  1:151992/139‑1 (MQ=255)                                            gTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCAt                                                                     >  2:549415/1‑139 (MQ=255)                                             ttCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCTAGCCATACGCCGCATAACGATAATGTTCATc                                                                    <  1:869581/139‑1 (MQ=255)                                                  tGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCa                                                               <  1:565681/139‑1 (MQ=255)                                                    aCGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATa                                                             <  1:283953/139‑1 (MQ=255)                                                    aCGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATa                                                             <  1:982926/139‑1 (MQ=255)                                                     cGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATAc                                                            <  2:177237/139‑1 (MQ=255)                                                             cGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGACATTa                                                    <  1:216034/139‑1 (MQ=255)                                                                    cTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCt                                             <  1:891961/139‑1 (MQ=255)                                                                          cGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATc                                       >  2:668562/1‑139 (MQ=255)                                                                           gcgCAATTAATCGGTTAGCGTATTCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATcc                                      >  2:868672/3‑139 (MQ=255)                                                                           gTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCAt                                                              >  1:968820/1‑115 (MQ=255)                                                                           gTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCAt                                                              <  2:968820/115‑1 (MQ=255)                                                                            tGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATAcgccg                                                                                       >  2:322253/1‑89 (MQ=255)                                                                            tGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATAcgccg                                                                                       <  1:322253/89‑1 (MQ=255)                                                                            tGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTc                                                                       >  1:48316/1‑105 (MQ=255)                                                                            tGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTc                                                                       >  2:966118/1‑105 (MQ=255)                                                                            tGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTc                                                                       <  2:48316/105‑1 (MQ=255)                                                                            tGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTc                                                                       <  1:966118/105‑1 (MQ=255)                                                                            tGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATAc                                                            <  2:667274/116‑1 (MQ=255)                                                                            tGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATAc                                                            >  1:667274/1‑116 (MQ=255)                                                                            tGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCt                                     >  2:100755/1‑139 (MQ=255)                                                                              cAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATa                                                                                            >  2:858601/1‑82 (MQ=255)                                                                              cAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATa                                                                                            <  1:858601/82‑1 (MQ=255)                                                                              cAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGc                                   >  1:605270/1‑139 (MQ=255)                                                                              cAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGc                                   >  1:179531/1‑139 (MQ=255)                                                                              cAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGc                                   >  1:733578/1‑139 (MQ=255)                                                                                  tAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGa                               <  1:549415/139‑1 (MQ=255)                                                                                         ttAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGa                               >  1:468211/1‑132 (MQ=255)                                                                                         ttAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGa                               <  2:468211/132‑1 (MQ=255)                                                                                             cGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCAt                                                                     <  1:546791/90‑1 (MQ=255)                                                                                             cGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCAt                                                                     >  2:546791/1‑90 (MQ=255)                                                                                               tAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACg                  <  2:1015117/139‑1 (MQ=255)                                                                                                 aTCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGcc                <  1:668562/139‑1 (MQ=255)                                                                                                  tCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGata                                         <  2:114431/113‑1 (MQ=255)                                                                                                  tCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGata                                         >  1:114431/1‑113 (MQ=255)                                                                                                      gCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGgaa           >  2:476578/1‑139 (MQ=255)                                                                                                       cAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGc                                                                                                          >  1:947476/1‑43 (MQ=255)                                                                                                       cAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGc                                                                                                          <  2:947476/43‑1 (MQ=255)                                                                                                             aaCGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAatgaatga    <  2:179531/139‑1 (MQ=255)                                                                                                             aaCGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAatgaatga    <  2:336474/139‑1 (MQ=255)                                                                                                             aaCGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAatgaatga    <  1:100755/139‑1 (MQ=255)                                                                                                               cGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAATGAATGACt  >  2:460847/1‑139 (MQ=255)                                                                                                                    tGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCt                                             >  1:426758/1‑91 (MQ=255)                                                                                                                    tGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCt                                             <  2:426758/91‑1 (MQ=255)                                                                                                                      cACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTc                                                                       >  2:698619/1‑63 (MQ=255)                                                                                                                      cACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTc                                                                       <  1:698619/63‑1 (MQ=255)                                                                                                                           tCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGata                                         >  2:831282/1‑88 (MQ=255)                                                                                                                           tCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGata                                         <  1:831282/88‑1 (MQ=255)                                                                                                                           tCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAg                                <  2:165405/97‑1 (MQ=255)                                                                                                                           tCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAg                                >  1:165405/1‑97 (MQ=255)                                                                                                                                   |                                                                                                                       CCGCTGTGGCAATTTCTGGCTACCATCCATCGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAATGAATGACT  >  NC_000913/1629783‑1630031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑