Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,735,897	T→C	Y174H (TAT→CAT)	sodB →	superoxide dismutase, Fe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,735,897	0	T	C	100.0%	148.8 / NA	40	Y174H (TAT→CAT)	sodB	superoxide dismutase, Fe
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/23);  total (17/23)

AAACAGCGATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTA  >  NC_000913/1735761‑1736027                                                                                                                                         |                                                                                                                                   aaaCAGCGATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCAt                                                                                                                                  <  1:133908/139‑1 (MQ=255)       cGATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCACTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAg                                                                                                                            >  1:863548/1‑139 (MQ=255)        gATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGc                                                                                                                           <  2:1009034/139‑1 (MQ=255)              aaaCTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCt                                                                                                                     <  1:363250/139‑1 (MQ=255)                           tttCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTggg                                                                                                                  >  2:902231/1‑129 (MQ=255)                           tttCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTggg                                                                                                                  <  1:902231/129‑1 (MQ=255)                            ttCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAAc                                                                                                       <  1:924034/139‑1 (MQ=255)                                            ggTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGa                                                                                       >  1:230472/1‑139 (MQ=255)                                                     cTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCg                                                                              <  2:393861/139‑1 (MQ=255)                                                     cTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCg                                                                              <  1:62416/139‑1 (MQ=255)                                                      ttgctaccGGTCCGACCCCGGTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTACTGGCCATCTGGAGCATTTCTGGGCTGTGGTGAACTGGAAATTCGTAACGAAAACTCTCGc                                                                             >  2:1049451/6‑139 (MQ=255)                                                      tGACCCCCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATTCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGc                                                                             <  1:688707/139‑1 (MQ=255)                                                      tGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGc                                                                             <  2:508439/139‑1 (MQ=255)                                                        accaccGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTg                                                                           <  1:771759/139‑1 (MQ=255)                                                        accaccGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTg                                                                           <  1:501832/139‑1 (MQ=255)                                                                        cGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGc                                                           <  2:899901/139‑1 (MQ=255)                                                                          ctgctgACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGc                                                                             <  2:50621/119‑1 (MQ=255)                                                                          ctgctgACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGc                                                                             >  1:50621/1‑119 (MQ=255)                                                                                  cGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCg                                                                              <  1:879933/110‑1 (MQ=255)                                                                                  cGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCg                                                                              >  2:879933/1‑110 (MQ=255)                                                                                                        tattaCATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTg                                                                           <  1:906043/91‑1 (MQ=255)                                                                                                        tattaCATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTg                                                                           >  2:906043/1‑91 (MQ=255)                                                                                                        tattaCATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTAt                           <  1:965790/139‑1 (MQ=255)                                                                                                        tattaCATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTAt                           <  2:83755/139‑1 (MQ=255)                                                                                                          ttaCATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCa                         >  1:632817/1‑139 (MQ=255)                                                                                                               tCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGa                                                                                                         <  1:795575/54‑1 (MQ=255)                                                                                                               tCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGa                                                                                                         >  2:795575/1‑54 (MQ=255)                                                                                                               tCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCt                    >  2:493215/1‑139 (MQ=255)                                                                                                                 gACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTa                   >  2:179066/1‑138 (MQ=255)                                                                                                                 gACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTa                   <  1:179066/138‑1 (MQ=255)                                                                                                                 gACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTAc                  >  2:20565/1‑139 (MQ=255)                                                                                                                  aCTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTAca                 >  1:329162/1‑138 (MQ=255)                                                                                                                       cGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTg                                                                           >  1:716844/1‑76 (MQ=255)                                                                                                                       cGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTg                                                                           <  2:716844/76‑1 (MQ=255)                                                                                                                         cAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGatca          <  2:230472/139‑1 (MQ=255)                                                                                                                            tGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGc                                                                             >  2:599554/1‑69 (MQ=255)                                                                                                                            tGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGc                                                                             <  1:599554/69‑1 (MQ=255)                                                                                                                                 gTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAAtctc                                                                               <  2:674163/62‑1 (MQ=255)                                                                                                                                 gTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAAtctc                                                                               >  1:674163/1‑62 (MQ=255)                                                                                                                                 gTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTa  >  2:546416/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                   AAACAGCGATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTA  >  NC_000913/1735761‑1736027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑