Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,071,304	A→G	intergenic (+93/‑13)	yeeP → / → isrC	pseudogene, CP4‑44 prophage, 50S ribosome‑binding GTPase family,Phage or Prophage Related, putative histone/novel sRNA, CP4‑44, putative prophage remnant

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,071,304	0	A	G	100.0%	193.7 / NA	51	intergenic (+93/‑13)	yeeP/isrC	pseudogene, CP4‑44 prophage, 50S ribosome‑binding GTPase family,Phage or Prophage Related, putative histone/novel sRNA, CP4‑44, putative prophage remnant
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/29);  total (22/29)

TCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGAACAACAGAACCA  >  NC_000913/2071173‑2071427                                                                                                                                    |                                                                                                                            tctGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAAt                                                                                                                      >  1:699686/1‑139 (MQ=255)  ctGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATa                                                                                                                     <  1:394092/139‑1 (MQ=255)    gTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATaaa                                                                                                                   >  1:546059/1‑139 (MQ=255)     ttGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATaaaa                                                                                                                  <  2:56558/139‑1 (MQ=255)      tGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAAc                                                                                                                 <  1:682764/139‑1 (MQ=255)        cccGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGa                                                                                                               >  2:257592/1‑139 (MQ=255)        cccGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGa                                                                                                               >  2:185528/1‑139 (MQ=255)         ccGTGCGGTATGGCACTGGATTTTCTTCTGAACCTGTCGTTACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGAt                                                                                                              <  2:741824/139‑1 (MQ=255)         ccGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGAt                                                                                                              <  1:257592/139‑1 (MQ=255)             gCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAat                                                                                                          <  2:662720/139‑1 (MQ=255)             gCGGTATGGAACTGGATTTTCTTCTGAACCGGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGTTAGAATAAAACGATCAat                                                                                                          >  2:809143/1‑139 (MQ=255)                      aaCTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTAtttt                                                                                                 >  2:860683/1‑139 (MQ=255)                          ggATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                             <  2:840161/139‑1 (MQ=255)                           gATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcga                                                                                            <  1:580132/139‑1 (MQ=255)                           gATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                             >  2:596463/1‑138 (MQ=255)                           gATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                             >  2:263282/1‑138 (MQ=255)                           gATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                             <  1:726343/138‑1 (MQ=255)                           gATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                             >  2:726343/1‑138 (MQ=255)                           gATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                             <  1:596463/138‑1 (MQ=255)                           gATTTTCTTCTGAACCTGTCGTGACTGATGCCCCCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                             <  1:263282/138‑1 (MQ=255)                            aTTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcgat                                                                                           <  2:1038169/139‑1 (MQ=255)                                      gAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATatc                                                                                 <  1:741273/139‑1 (MQ=255)                                            gTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATAtcgatcga                                                                           <  1:384166/139‑1 (MQ=255)                                                  aCTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATATCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATGGATAAGCt                                                                     <  2:699686/139‑1 (MQ=255)                                                  aCTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCt                                                                     <  2:546059/139‑1 (MQ=255)                                                           ctccctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAAc                                                            >  1:235698/1‑139 (MQ=255)                                                                    tctGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCt                                                                     <  1:531433/121‑1 (MQ=255)                                                                    tctGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCt                                                                     >  2:531433/1‑121 (MQ=255)                                                                      tGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTa                                                 <  1:76464/139‑1 (MQ=255)                                                                        aGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATaaaa                                                                                                                  >  2:907359/1‑72 (MQ=255)                                                                        aGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATaaaa                                                                                                                  <  1:907359/72‑1 (MQ=255)                                                                        aGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                             <  1:525719/93‑1 (MQ=255)                                                                        aGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                             >  2:525719/1‑93 (MQ=255)                                                                              cTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                             >  2:1026220/1‑87 (MQ=255)                                                                              cTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                             <  1:1026220/87‑1 (MQ=255)                                                                                  caAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGAt                                     >  1:28463/1‑139 (MQ=255)                                                                                  caAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGAt                                     <  1:1011088/139‑1 (MQ=255)                                                                                        cACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATcgatcgat                                                                          <  2:510007/96‑1 (MQ=255)                                                                                        cACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATcgatcgat                                                                          >  1:510007/1‑96 (MQ=255)                                                                                                   ttACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACAtt                    <  2:458294/139‑1 (MQ=255)                                                                                                       tGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAATGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACGTTTGTCAGTAACATGca                                          <  2:632236/113‑1 (MQ=255)                                                                                                       tGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTTAGTAACATGca                                          >  1:632236/1‑113 (MQ=255)                                                                                                                     gTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATcgatcgat                                                                          <  1:286905/67‑1 (MQ=255)                                                                                                                     gTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATcgatcgat                                                                          >  2:286905/1‑67 (MQ=255)                                                                                                                     gTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGAACAACAGAACca  >  2:188710/1‑139 (MQ=255)                                                                                                                      tGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCa                                                    >  2:585076/1‑88 (MQ=255)                                                                                                                      tGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCa                                                    <  1:585076/88‑1 (MQ=255)                                                                                                                       gCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAAc                                               >  2:599121/1‑92 (MQ=255)                                                                                                                       gCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAAc                                               <  1:599121/92‑1 (MQ=255)                                                                                                                       gCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGaac            <  1:351542/127‑1 (MQ=255)                                                                                                                       gCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGaac            >  2:351542/1‑127 (MQ=255)                                                                                                                                    |                                                                                                                            TCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGAACAACAGAACCA  >  NC_000913/2071173‑2071427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑