Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,244,769	A→G	intergenic (+23/+9)	yeiG → / ← cirA	S‑formylglutathione hydrolase/colicin IA outer membrane receptor and translocator, ferric iron‑catecholate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,244,769	0	A	G	100.0%	231.9 / NA	61	intergenic (+23/+9)	yeiG/cirA	S‑formylglutathione hydrolase/colicin IA outer membrane receptor and translocator, ferric iron‑catecholate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/25);  total (36/25)

CGTATTCAGCCGGGATATGATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTAGTC  >  NC_000913/2244654‑2244901                                                                                                                    |                                                                                                                                     cGTATTCAGCCGGGATATGATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGtc                                                                                                                                    >  1:328373/1‑118 (MQ=255)         gCCGGGATATGATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCa                                                                                                       <  1:775829/139‑1 (MQ=255)                        caGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGAcgtc                                                                                        >  2:517461/1‑139 (MQ=255)                        caGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGAAGTATTTACTGAAGGGAAAGTCCGCCCGGTTCGCCGTGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGAcgtc                                                                                        >  2:646950/1‑139 (MQ=255)                         aGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGAcgtcc                                                                                       <  2:933518/139‑1 (MQ=255)                             actactTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCt                                                                                   >  2:66599/1‑139 (MQ=255)                              ctactTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGACGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCtt                                                                                  >  2:29765/1‑139 (MQ=255)                                   tCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTta                                                                             >  2:372365/1‑139 (MQ=255)                                   tCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTta                                                                             >  1:931903/1‑139 (MQ=255)                                     aTCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTtata                                                                           >  2:1048174/1‑139 (MQ=255)                                     aTCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTtata                                                                           >  2:456954/1‑139 (MQ=255)                                         ccTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTg                                                                       >  2:851472/1‑139 (MQ=255)                                            ctTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCa                                                                                                       <  2:137660/104‑1 (MQ=255)                                            ctTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCa                                                                                                       <  2:529264/104‑1 (MQ=255)                                            ctTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCa                                                                                                       >  1:529264/1‑104 (MQ=255)                                            ctTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCa                                                                                                       >  1:137660/1‑104 (MQ=255)                                                    gagGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACg                                                            <  1:29765/139‑1 (MQ=255)                                                       gATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCtcatc                                                                                                                            <  1:539041/72‑1 (MQ=255)                                                       gATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCtcatc                                                                                                                            >  2:539041/1‑72 (MQ=255)                                                       gATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCAc                                                                                                            <  2:653942/88‑1 (MQ=255)                                                       gATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCAc                                                                                                            >  1:653942/1‑88 (MQ=255)                                                       gATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTtat                                                                            <  2:851306/120‑1 (MQ=255)                                                       gATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTtat                                                                            >  1:851306/1‑120 (MQ=255)                                                       gATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACt                                                         >  1:651101/1‑139 (MQ=255)                                                          cACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTgag                                                      >  2:369749/1‑139 (MQ=255)                                                                    tCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCg                                            >  1:500060/1‑139 (MQ=255)                                                                      cATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGcc                                          <  1:487220/139‑1 (MQ=255)                                                                       aTGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCa                                                                                                       <  1:282974/77‑1 (MQ=255)                                                                       aTGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCa                                                                                                       >  2:282974/1‑77 (MQ=255)                                                                       aTGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCa                                         <  2:651101/139‑1 (MQ=255)                                                                            cAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGtt                                    <  1:369749/139‑1 (MQ=255)                                                                             aGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATc                                                                                                               <  2:484637/63‑1 (MQ=255)                                                                             aGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATc                                                                                                               >  1:484637/1‑63 (MQ=255)                                                                               tATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCa                                                                                                       <  2:466074/69‑1 (MQ=255)                                                                               tATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCa                                                                                                       >  1:466074/1‑69 (MQ=255)                                                                               tATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAgg                                      >  2:296162/1‑134 (MQ=255)                                                                               tATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAgg                                      <  1:296162/134‑1 (MQ=255)                                                                               tATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAg                                 >  2:1048746/1‑139 (MQ=255)                                                                                       gAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTg                         >  2:913201/1‑139 (MQ=255)                                                                                         aGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGa                                                                                                                         >  1:1056106/1‑41 (MQ=255)                                                                                         aGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGa                                                                                                                         <  2:1056106/41‑1 (MQ=255)                                                                                         aGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCa                                                                                                             >  2:477713/1‑53 (MQ=255)                                                                                         aGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCa                                                                                                             <  1:477713/53‑1 (MQ=255)                                                                                         aGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAgg                                      >  2:722844/1‑124 (MQ=255)                                                                                         aGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAgg                                      <  1:722844/124‑1 (MQ=255)                                                                                           tGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCAc                                                                                                            <  2:731334/52‑1 (MQ=255)                                                                                           tGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCAc                                                                                                            >  2:89347/1‑52 (MQ=255)                                                                                           tGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCAc                                                                                                            <  1:89347/52‑1 (MQ=255)                                                                                           tGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCAc                                                                                                            >  1:731334/1‑52 (MQ=255)                                                                                           tGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTgcgcg                     >  2:4334/1‑139 (MQ=255)                                                                                           tGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTgcgcg                     >  2:281599/1‑139 (MQ=255)                                                                                             aaaGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGtt                                    <  2:362933/122‑1 (MQ=255)                                                                                             aaaGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGtt                                    >  1:362933/1‑122 (MQ=255)                                                                                                 tCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGttt               <  1:1048174/139‑1 (MQ=255)                                                                                                   cGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGa             >  1:286888/1‑139 (MQ=255)                                                                                                   cGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGa             >  1:851426/1‑139 (MQ=255)                                                                                                     cccGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCtt                                                                                  <  1:1009556/68‑1 (MQ=255)                                                                                                     cccGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCtt                                                                                  >  2:1009556/1‑68 (MQ=255)                                                                                                     cccGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCa                               >  2:372379/1‑119 (MQ=255)                                                                                                     cccGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCa                               <  1:372379/119‑1 (MQ=255)                                                                                                              ccGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTAGTc  <  1:210925/139‑1 (MQ=255)                                                                                                                    |                                                                                                                                     CGTATTCAGCCGGGATATGATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTAGTC  >  NC_000913/2244654‑2244901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑