Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	420,175	G→A	L195L (CTG→CTA)	brnQ →	branched‑chain amino acid transport system 2 carrier protein, LIV‑II transport system for Ile, Leu, and Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	420,175	0	G	A	100.0%	126.9 / NA	37	L195L (CTG→CTA)	brnQ	branched‑chain amino acid transport system 2 carrier protein, LIV‑II transport system for Ile, Leu, and Val
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/17);  total (20/17)

CGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGTTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACT  >  NC_000913/420063‑420310                                                                                                                 |                                                                                                                                        cgcTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGgt                                                                                                               >  2:731424/1‑139 (MQ=255) cgcTGGTCACCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGTTACGCTGGGCGCAATGgt                                                                                                               >  1:742517/1‑139 (MQ=255)    tGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGtt                                                                                                            <  2:996983/139‑1 (MQ=255)         aTCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGAGTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTa                                                                                                       <  1:731424/139‑1 (MQ=255)                     gCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTa                                                                                           >  2:880087/1‑139 (MQ=255)                      ccGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTaa                                                                                          >  2:81502/1‑139 (MQ=255)                        gCTCTTGTCTGGCCGGCGGGTTCTAGCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCACTGGTGTTTGGTATCGTTATTGTTAAcg                                                                                        <  1:714476/139‑1 (MQ=255)                          tATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAAcgcg                                                                                      <  2:511706/139‑1 (MQ=255)                           aTTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCaa                                                                                                                   >  2:478825/1‑109 (MQ=255)                           aTTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCaa                                                                                                                   <  1:478825/109‑1 (MQ=255)                                    ccGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTtctc                                                                            >  1:695119/1‑139 (MQ=255)                                      ggcggGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGt                                                                          <  1:880087/139‑1 (MQ=255)                                        cggGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTgg                                                                        >  1:837331/1‑139 (MQ=255)                                        cggGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTgg                                                                        >  1:211523/1‑139 (MQ=255)                                              cTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTAc                                                                  <  1:830580/139‑1 (MQ=255)                                                     cACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGgtgt                                                                                                             >  2:280133/1‑89 (MQ=255)                                                     cACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGgtgt                                                                                                             <  1:280133/89‑1 (MQ=255)                                                     cACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAgcg                                                           >  2:968100/1‑139 (MQ=255)                                                      aCGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAgcgc                                                          >  2:422165/1‑139 (MQ=255)                                                         gCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTgg                                                                                                         <  2:4450/89‑1 (MQ=255)                                                         gCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTgg                                                                                                         >  1:4450/1‑89 (MQ=255)                                                                  gCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGAccc                                              >  2:547155/1‑139 (MQ=255)                                                                      aTCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTAt                                                                                                <  1:933579/85‑1 (MQ=255)                                                                      aTCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTAt                                                                                                >  2:933579/1‑85 (MQ=255)                                                                            aCGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACACTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGTTATACCGt                                    >  2:221854/1‑139 (MQ=255)                                                                                  cGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCaa                                                                                                                   >  2:89987/1‑54 (MQ=255)                                                                                  cGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCaa                                                                                                                   <  1:89987/54‑1 (MQ=255)                                                                                  cGTTTTCTAACGGCTTCGTTAACGGCTATCTAACCATGGATACACTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGTTATACCGTCTGGGc                              <  1:221854/139‑1 (MQ=255)                                                                                            cGGCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGTTATACCGTCTGGGCTGGCCTGATg                    >  1:794087/1‑139 (MQ=255)                                                                                              gCTTCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGTTATACCGTCTGGGCTGGCCTGATGGc                  <  2:695119/139‑1 (MQ=255)                                                                                                 tCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAgcgc                                                          <  2:661021/96‑1 (MQ=255)                                                                                                 tCGTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAgcgc                                                          >  1:661021/1‑96 (MQ=255)                                                                                                   gTTAACGGCTATCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGTTATACCGTCTGGGCTGGCCTGATGGCGGgtg             <  1:946175/139‑1 (MQ=255)                                                                                                             aTCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGcgcg                                                         <  2:828273/85‑1 (MQ=255)                                                                                                             aTCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGcgcg                                                         >  1:828273/1‑85 (MQ=255)                                                                                                             aTCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGTTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGAc   >  2:156978/1‑139 (MQ=255)                                                                                                              tCTAACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGTTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGAct  <  1:81502/139‑1 (MQ=255)                                                                                                                 |                                                                                                                                        CGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGTTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACT  >  NC_000913/420063‑420310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑