| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,649,905 | G→A | P795S (CCG→TCG) | yfhM ← | bacterial alpha2‑macroglobulin colonization factor ECAM, anti‑host protease defense factor, periplasmic inner membrane‑anchored lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,649,905 | 0 | G | A | 95.3% | 130.8 / ‑6.1 | 43 | P795S (CCG→TCG) | yfhM | bacterial alpha2‑macroglobulin colonization factor ECAM, anti‑host protease defense factor, periplasmic inner membrane‑anchored lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (26/15); minor base C (0/2); total (26/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGC > NC_000913/2649773‑2650034 | cgGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCAGAGCGACCCTTc > 1:717050/1‑139 (MQ=255) aTTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTc < 2:762106/139‑1 (MQ=255) tATCGCCAGGACGTACCACCAGCGTACTTAAACACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGAc < 2:907246/139‑1 (MQ=255) gACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGAcc < 1:230254/114‑1 (MQ=255) gACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGAcc > 2:230254/1‑114 (MQ=255) gACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGAc < 2:853230/130‑1 (MQ=255) gACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGAc > 1:853230/1‑130 (MQ=255) cGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACccgcc > 2:26227/1‑139 (MQ=255) cTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTg > 1:730290/1‑139 (MQ=255) aaaTACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGCCCCTTCACTGGACTCGACCATCGCATAACCTTTCCCCGCCGTTGgcg < 1:26227/139‑1 (MQ=255) aGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGgcgcagc > 2:663085/1‑134 (MQ=255) aGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCgcagc < 1:663085/137‑1 (MQ=255) aTCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACTGCGACCCTTCACTGGACTCCACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGata > 2:911103/1‑139 (MQ=255) aTCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCAc > 1:496740/1‑92 (MQ=255) aTCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCAc < 2:496740/92‑1 (MQ=255) aTCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGata > 2:887412/1‑139 (MQ=255) aTCATGACGAATCGAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCGACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGata > 2:740538/1‑139 (MQ=255) cATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATg > 1:622762/1‑139 (MQ=255) cATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATg < 1:887412/139‑1 (MQ=255) cATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTGGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACGGAACTCGACCATCGCAAAACTTTTACCCGCCGTTGGCGCGGCGATATg < 2:717050/139‑1 (MQ=255) gACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCGCTTGAGCCCGAACATCGATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGAcc < 2:729947/98‑1 (MQ=255) gACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGAcc < 2:60191/98‑1 (MQ=255) gACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGAcc > 1:729947/1‑98 (MQ=255) gACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGAcc > 1:60191/1‑98 (MQ=255) gACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCaa > 1:838817/1‑139 (MQ=255) ttCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCATCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTaa > 2:753550/1‑139 (MQ=255) taGGTTTTTCCGACAGGAATCGTCAGATCCAGCCTTTGAGCCCGAACATCAACATCTCGCCCCCACAGCGACCCTTCACTTGACCCGACCATCGCATACCTTGCGCCCGCCGTTGGCGCGGCGATATGCAACTTAATgg < 2:565487/138‑1 (MQ=255) aGGTTTTATCGACCGGAATGGTCAGATCCACCCCTTGAGCCCGAACATCAACCTCTTGCCCCCACAGCGCCCTTTCGCTGGACTCGACCATCGCATACCCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGgt < 2:724104/139‑1 (MQ=255) tATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGcc > 1:724104/1‑139 (MQ=255) tCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGc > 1:307977/1‑139 (MQ=255) cAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCt > 1:646961/1‑139 (MQ=255) tCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCgcgg > 2:392465/1‑94 (MQ=255) tCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCgcgg < 1:392465/94‑1 (MQ=255) cccTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTAcc > 1:779222/1‑75 (MQ=255) cccTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGt < 1:97652/139‑1 (MQ=255) cccTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACGGGACTCGACCATCGCATAACCTTTAcc < 2:779222/75‑1 (MQ=255) aGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACccgccaacg < 2:95158/77‑5 (MQ=255) aGCCCGAACATCAATCTCTTGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACccgccaacg > 1:95158/1‑73 (MQ=255) ctTGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACggtcgggtcg > 1:251535/1‑139 (MQ=255) tGCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCa > 1:127899/1‑139 (MQ=255) gCCACCACAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCAc > 1:770239/1‑139 (MQ=255) ccaccaCAGCGACCCTTCACTGGACTCGACCATCGCa < 2:386460/37‑1 (MQ=255) ccaccaCAGCGACCCTTCACTGGACTCGACCATCGCa > 1:386460/1‑37 (MQ=255) accacAGCGACCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTgc > 1:67131/1‑139 (MQ=255) | CGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGC > NC_000913/2649773‑2650034 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |