Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 459,628 G→A A247A (GCG→GCA)  lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913459,6280GA100.0% 84.9 / NA 26A247A (GCG→GCA) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/13);  total (13/13)

GAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCG  >  NC_000913/459494‑459762
                                                                                                                                      |                                                                                                                                      
gAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCgg                                                                                                                                    >  2:600441/1‑139 (MQ=255)
    tCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGa                                                                                                                                <  2:473602/139‑1 (MQ=255)
       aTCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGaaaa                                                                                                                             <  2:39703/139‑1 (MQ=255)
                           cGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGAGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCaaaa                                                                                                         >  2:225019/1‑139 (MQ=255)
                                                 aGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGaa                                                                                   <  1:600441/139‑1 (MQ=255)
                                                                                 cTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGa                                                                                                     <  2:590289/89‑1 (MQ=255)
                                                                                 cTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGa                                                                                                     >  1:590289/1‑89 (MQ=255)
                                                                                    aaCGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGa                                                >  1:1079658/1‑139 (MQ=255)
                                                                                    aaCGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGa                                                >  2:137449/1‑139 (MQ=255)
                                                                                        aGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCt                                            >  2:356954/1‑139 (MQ=255)
                                                                                        aGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCt                                            >  2:1004669/1‑139 (MQ=255)
                                                                                          cAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGaa                                                                                            >  1:208033/1‑89 (MQ=255)
                                                                                          cAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGaa                                                                                            <  2:208033/89‑1 (MQ=255)
                                                                                          cAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGa                                          <  1:356954/139‑1 (MQ=255)
                                                                                          cAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGa                                          >  2:126423/1‑139 (MQ=255)
                                                                                                aaaGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatga                                    <  2:1103651/139‑1 (MQ=255)
                                                                                                 aaGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatgat                                   <  1:126423/139‑1 (MQ=255)
                                                                                                  aGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatgatg                                  <  1:137449/139‑1 (MQ=255)
                                                                                                  aGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatgat                                   <  2:37147/138‑1 (MQ=255)
                                                                                                  aGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatgat                                   >  1:37147/1‑138 (MQ=255)
                                                                                                             aagaaCTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTc                       >  2:1064839/1‑139 (MQ=255)
                                                                                                                 aCTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAg                                                            >  1:642368/1‑98 (MQ=255)
                                                                                                                 aCTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAg                                                            <  2:642368/98‑1 (MQ=255)
                                                                                                                 aCTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCa                   >  2:775184/1‑139 (MQ=255)
                                                                                                                        gAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGTAGAAGCGa            <  1:628008/139‑1 (MQ=255)
                                                                                                                                  acgCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCg  <  2:761096/139‑1 (MQ=255)
                                                                                                                                   cgcaCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTg    <  1:809239/136‑1 (MQ=255)
                                                                                                                                   cgcaCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTg    >  2:809239/1‑136 (MQ=255)
                                                                                                                                      |                                                                                                                                      
GAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCG  >  NC_000913/459494‑459762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑