Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,938,533	C→T	R103W (CGG→TGG)	rbsR →	transcriptional repressor of ribose metabolism

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,938,533	0	C	T	100.0%	188.1 / NA	54	R103W (CGG→TGG)	rbsR	transcriptional repressor of ribose metabolism
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/25);  total (29/25)

CACATACCATTGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGG  >  NC_000913/3938402‑3938668                                                                                                                                    |                                                                                                                                        cacaTACCATTGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGaa                                                                                                                                  >  1:61604/1‑139 (MQ=255)      aCCATTGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGTCGTTGAACGCAGCTGCTTCGAACGCGGGTATAGTCTCGTCCTTTGCAATACTGAAGGCGATGAACAGTGGATGAATCGCa                                                                                                                             >  1:662932/1‑139 (MQ=255)      aCCATTGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCa                                                                                                                             >  1:906592/1‑139 (MQ=255)          ttGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCt                                                                                                                         >  1:556883/1‑139 (MQ=255)          ttGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCt                                                                                                                         >  1:478075/1‑139 (MQ=255)                  ttGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGc                                                                                                                 >  1:240165/1‑139 (MQ=255)                    gATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTg                                                                                                               <  2:61604/139‑1 (MQ=255)                    gATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTg                                                                                                               >  2:852528/1‑139 (MQ=255)                       cACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATg                                                                                                            >  2:917821/1‑139 (MQ=255)                                  ccAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGt                                                                                                 >  2:506250/1‑139 (MQ=255)                                      tCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGAt                                                                                             >  2:55995/1‑139 (MQ=255)                                             tATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGc                                                                                      <  2:906592/139‑1 (MQ=255)                                                  aGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTa                                                                                 <  2:418952/139‑1 (MQ=255)                                                    aaCTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACt                                                                               >  2:685931/1‑139 (MQ=255)                                                         gTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATgg                                                                                           >  2:61815/1‑122 (MQ=255)                                                         gTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATgg                                                                                           <  1:61815/122‑1 (MQ=255)                                                         gTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCa                                                                          >  2:995266/1‑139 (MQ=255)                                                         gTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCa                                                                          >  2:171346/1‑139 (MQ=255)                                                              tGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCa                                                                                                                             <  2:264922/83‑1 (MQ=255)                                                              tGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCa                                                                                                                             >  1:264922/1‑83 (MQ=255)                                                              tGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAACCGCTGATGCAAAAACGCGTTGATGGCt                                                                                         <  2:376110/119‑1 (MQ=255)                                                              tGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAACCGCTGATGCAAAAACGCGTTGATGGCt                                                                                         >  1:376110/1‑119 (MQ=255)                                                                          cAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCt                                                         <  2:860703/139‑1 (MQ=255)                                                                          cAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCt                                                         <  1:927411/139‑1 (MQ=255)                                                                          cAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCt                                                         <  1:1003758/139‑1 (MQ=255)                                                                                ctTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCCCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAAGCTTCGCGt                                                   >  1:657102/1‑139 (MQ=255)                                                                                            ttATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCCAAACGCATCAACCTTCGCGTGAAATCATGCaa                                       <  2:657102/139‑1 (MQ=255)                                                                                              ataGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACg                                     <  2:380493/139‑1 (MQ=255)                                                                                                  tctcGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCa                                        >  1:675425/1‑132 (MQ=255)                                                                                                  tctcGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCa                                        <  2:675425/132‑1 (MQ=255)                                                                                                      gTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCaaa                                                                                                        <  2:592768/64‑1 (MQ=255)                                                                                                      gTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCaaa                                                                                                        >  1:592768/1‑64 (MQ=255)                                                                                                      gTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGa                             >  1:200561/1‑139 (MQ=255)                                                                                                      gTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGa                             >  2:770516/1‑139 (MQ=255)                                                                                                       tCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCTATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGAc                            <  2:662932/139‑1 (MQ=255)                                                                                                       tCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGAc                            <  1:55995/139‑1 (MQ=255)                                                                                                          tttGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGt                         <  1:917821/139‑1 (MQ=255)                                                                                                            tGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGc                       >  1:205335/1‑139 (MQ=255)                                                                                                              cAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCt                     <  2:240165/139‑1 (MQ=255)                                                                                                                aTACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTAc                   <  2:205335/139‑1 (MQ=255)                                                                                                                  aCCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACtg                 <  1:770516/139‑1 (MQ=255)                                                                                                                  aCCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACtg                 <  1:515822/139‑1 (MQ=255)                                                                                                                  aCCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACtg                 <  1:506250/139‑1 (MQ=255)                                                                                                                    cGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACtgtg               >  1:157512/1‑139 (MQ=255)                                                                                                                      aaGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTTCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGt                         <  1:656632/127‑1 (MQ=255)                                                                                                                      aaGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGt                         >  2:656632/1‑127 (MQ=255)                                                                                                                      aaGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGtgat             >  1:385408/1‑139 (MQ=255)                                                                                                                      aaGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGtgat             >  2:375649/1‑139 (MQ=255)                                                                                                                      aaGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGtgat             >  1:62542/1‑139 (MQ=255)                                                                                                                             tGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTa                                                                                 >  2:207625/1‑64 (MQ=255)                                                                                                                             tGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTa                                                                                 <  1:207625/64‑1 (MQ=255)                                                                                                                             tGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTAc                   >  1:286993/1‑126 (MQ=255)                                                                                                                             tGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTAc                   <  2:286993/126‑1 (MQ=255)                                                                                                                                 cAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTggg  <  1:685931/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        CACATACCATTGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGG  >  NC_000913/3938402‑3938668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑