Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,351,093	G→A	G190G (GGC→GGT)	dcuS ←	sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,351,093	0	G	A	100.0%	186.3 / NA	53	G190G (GGC→GGT)	dcuS	sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/29);  total (24/29)

CTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATA  >  NC_000913/4350966‑4351220                                                                                                                                 |                                                                                                                                cttTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATAc                                                                                                                      <  1:605985/139‑1 (MQ=255)    tGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGa                                                                                                                   <  1:223135/139‑1 (MQ=255)          aCTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGAc                                                                                                                  >  2:164322/1‑134 (MQ=255)          aCTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGAc                                                                                                                  <  1:164322/134‑1 (MQ=255)          aCTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGAt                                                                                                             >  1:847674/1‑139 (MQ=255)          aCTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGAt                                                                                                             >  2:730484/1‑139 (MQ=255)           cTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATa                                                                                                            <  1:26147/139‑1 (MQ=255)            tGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATa                                                                                                            <  1:436306/139‑1 (MQ=255)            tGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATaa                                                                                                           >  1:1032481/1‑139 (MQ=255)            tGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATaa                                                                                                           <  1:1026593/139‑1 (MQ=255)            tGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATaa                                                                                                           >  1:533005/1‑139 (MQ=255)                    ggCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAg                                                                                                   <  2:867207/139‑1 (MQ=255)                      ccTGGCGTTGCTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCg                                                                                                 <  1:1031887/139‑1 (MQ=255)                        tGGCGTTGCTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGAc                                                                                               <  1:730484/139‑1 (MQ=255)                        tGGCGTTGCTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGAc                                                                                               <  2:847674/139‑1 (MQ=255)                                cTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCa                                                                                                                >  2:105797/1‑114 (MQ=255)                                cTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCa                                                                                                                <  1:105797/114‑1 (MQ=255)                                cTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCg                                                                                                 >  2:1033936/1‑129 (MQ=255)                                cTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCg                                                                                                 <  1:1033936/129‑1 (MQ=255)                                cTCAAACAGCCTGGAGATTTAGTA‑GGGTTCCAGGCTGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAACATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTg                                                                                       <  2:273358/139‑1 (MQ=255)                                     aCAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTg                                                                                  >  2:685573/1‑139 (MQ=255)                                          gTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTggg                                                                             >  1:249659/1‑139 (MQ=255)                                          gTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTggg                                                                             >  1:400314/1‑139 (MQ=255)                                                   tCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGAc                                                                                               >  2:268018/1‑112 (MQ=255)                                                   tCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGAc                                                                                               <  1:268018/112‑1 (MQ=255)                                                    cGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCt                                                                   <  2:269949/139‑1 (MQ=255)                                                    cGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCt                                                                   <  1:468906/139‑1 (MQ=255)                                                              ccAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATAcc                                                                                                                     <  2:855802/80‑1 (MQ=255)                                                              ccAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATAcc                                                                                                                     >  1:855802/1‑80 (MQ=255)                                                                      aaaaGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCg                                                  >  2:975423/1‑139 (MQ=255)                                                                      aaaaGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCg                                                  >  1:668682/1‑139 (MQ=255)                                                                              ttttttCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATATTACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACg                                          <  2:668682/139‑1 (MQ=255)                                                                                   tCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAAt                                     <  2:249659/139‑1 (MQ=255)                                                                                   tCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAAt                                     <  1:685573/139‑1 (MQ=255)                                                                                            tAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTcaca                                                                        >  2:1074353/1‑95 (MQ=255)                                                                                            tAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTcaca                                                                        <  1:1074353/95‑1 (MQ=255)                                                                                                cAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGAttt                        <  2:916531/139‑1 (MQ=255)                                                                                                   aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGa                                                                                                <  2:48529/64‑1 (MQ=255)                                                                                                   aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGa                                                                                                >  1:48529/1‑64 (MQ=255)                                                                                                   aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGAc                                                                                               >  1:761154/1‑65 (MQ=255)                                                                                                   aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGAc                                                                                               <  2:761154/65‑1 (MQ=255)                                                                                                   aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCa                     >  2:137943/1‑139 (MQ=255)                                                                                                           tGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGAt             <  1:187728/139‑1 (MQ=255)                                                                                                               aaTCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGccacc                                             <  2:570916/103‑1 (MQ=255)                                                                                                               aaTCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGccacc                                             >  1:570916/1‑103 (MQ=255)                                                                                                               aaTCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGttt                               >  2:356347/1‑117 (MQ=255)                                                                                                               aaTCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGttt                               <  1:356347/117‑1 (MQ=255)                                                                                                                    gTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTaaa    >  2:513862/1‑139 (MQ=255)                                                                                                                     tCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAAt   >  1:853991/1‑139 (MQ=255)                                                                                                                     tCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAAt   >  2:139776/1‑139 (MQ=255)                                                                                                                      ccGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATa  <  2:179057/139‑1 (MQ=255)                                                                                                                       cGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGa              <  2:1040474/126‑1 (MQ=255)                                                                                                                       cGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGa              >  1:1040474/1‑126 (MQ=255)                                                                                                                                 |                                                                                                                                CTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTA‑GGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATA  >  NC_000913/4350966‑4351220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑