Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	602,603	G→A	L215L (CTG→CTA)	pheP →	phenylalanine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	602,603	0	G	A	100.0%	61.2 / NA	19	L215L (CTG→CTA)	pheP	phenylalanine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/12);  total (7/12)

GATCGGCTTTGGCCTGTGGCTGCTGTTTTCTGGTCACGGCGGCGAGAAAGCCAGTATCGACAACCTCTGGCGCTACGGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTGGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCA  >  NC_000913/602483‑602715                                                                                                                         |                                                                                                                 gATCGGCTTTGGCCTGTGGCTGCTGTTTTCTGGTCACGGCGGCGAGAAAGCCAGTATCGACAACCTCTGGCGCTACGGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTAGCGGTAATTATGTTCTcc                                                                                                <  1:8539/139‑1 (MQ=255)   tCGGCTTTGGCCTGTGGCTGCTGTTTTCTGGTCACGGCGGCGAGAAAGCCAGTATCGACAACCTCTGGCGCTACGGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTAGCGGTAATTATGTTCTCCtt                                                                                              <  1:339353/139‑1 (MQ=255)     ggCTTTGGCCTGTGGCTGCTGTTTTCTGGTCACGGCGGCGAGAAAGCCAGTATCGACAACCTCTGGCGCTACGGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTAGCGGTAATTATGTTCTCCTTcg                                                                                            >  2:414284/1‑139 (MQ=255)      gCTTTGGTCTGTGGCTGCTGTTTTCTGGTCACGGCGGCGAGAAAGCCAGTATCGACAACCTCTGGCGCTACGGTGGTTTCTTCGCCGCCGGCTGGAATGGGCTGATTTTGTCGCTAGCGGTAATTATGTTCTCCTTcgg                                                                                           <  1:213557/139‑1 (MQ=255)        tttGGCCTGTGGCTGCTGTTTTCTGGTCACGGCGGCGAGAAAGCCAGTATCGACAACCTCTGGCGCTACGGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTAGCGGTAATTATGTTCTCCTTcggcg                                                                                         >  1:287859/1‑139 (MQ=255)                 tgGCTGCTGTTTTCTGGTCACGGCGGCGAGAAAGCCAGTATCGACAACCTCTGGCGCTACGGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTAGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGc                                                                                >  1:40646/1‑139 (MQ=255)                 tgGCTGCTGTTTTCTGGTCACGGCGGCGAGAAAGCCAGTATCGACAACCTCTGGCGCTACGGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTAGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGc                                                                                >  2:1049730/1‑139 (MQ=255)                     tgctgTTTTCTGGTCACGGCGGCGAGAAAGCCAGTATCGACAACCTCTGGCGCTACGGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTAGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGAt                                                                            <  2:680360/139‑1 (MQ=255)                          ttttCTGGTCACGGCGGCGAGAAAGCCAGTATCGACAACCTCTGGCGCTACGGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTAGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGa                                                                       <  2:40646/139‑1 (MQ=255)                           tttCTGGTCACGGCGGCGAGAAAGCCAGTATCGACAACCTCTGGCGCTACGGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTAGCgg                                                                                                              <  1:1107513/99‑1 (MQ=255)                           tttCTGGTCACGGCGGCGAGAAAGCCAGTATCGACAACCTCTGGCGCTACGGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTAGCgg                                                                                                              >  2:1107513/1‑99 (MQ=255)                                    aCGGCGGCGAGAAAGCCAGTATCGACAACCTCTGGCGCTACGGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTAGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGc                                                             >  1:86597/1‑139 (MQ=255)                                             agaAAGCCAGTATCGACAACCTCTGGCGCTACGGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTAGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAgcgcg                                                    <  1:602486/139‑1 (MQ=255)                                             agaAAGCCAGTATCGACAACCTCTGGCGCTACGGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTAGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAgcgcg                                                    <  2:256674/139‑1 (MQ=255)                                                            aCAACCTCTGGCGCTACGGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTAGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAg                                     >  2:887714/1‑139 (MQ=255)                                                                                ggtTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTAGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATc                 <  2:287859/139‑1 (MQ=255)                                                                                  tttCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTAGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAg               <  1:916790/139‑1 (MQ=255)                                                                                   ttcttcGCCACCGGCTGGAATGGGCTGATTTTGTCGCTAGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAgg              <  1:414284/139‑1 (MQ=255)                                                                                               ggCTGGAATGGGCTGATTTTGTCGCTAGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCa  <  1:892649/139‑1 (MQ=255)                                                                                                                         |                                                                                                                 GATCGGCTTTGGCCTGTGGCTGCTGTTTTCTGGTCACGGCGGCGAGAAAGCCAGTATCGACAACCTCTGGCGCTACGGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTGGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCA  >  NC_000913/602483‑602715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑