Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	698,107	A→G	G357G (GGT→GGC)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	698,107	0	A	G	100.0%	264.4 / NA	68	G357G (GGT→GGC)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/32);  total (36/32)

GCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAA  >  NC_000913/697975‑698240                                                                                                                                     |                                                                                                                                      gcgTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGccgccg                                                                                                                                 <  2:18802/139‑1 (MQ=255)            cGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTtcatc                                                                                                                      <  2:397175/139‑1 (MQ=255)                      tGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCa                                                                                                            <  2:390052/139‑1 (MQ=255)                        aCATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCAcc                                                                                                          >  2:1021711/1‑139 (MQ=255)                        aCATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCAcc                                                                                                          >  2:200249/1‑139 (MQ=255)                         cATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCg                                                                                                         >  2:322221/1‑139 (MQ=255)                         cATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCg                                                                                                         >  1:600281/1‑139 (MQ=255)                          aTCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCgg                                                                                                        <  1:322221/139‑1 (MQ=255)                             gCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACa                                                                                                     >  2:621495/1‑139 (MQ=255)                              cTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCTGAACCTTCACCGGACAg                                                                                                    <  2:485818/139‑1 (MQ=255)                                ttgttgGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCa                                                                                                  <  2:13207/139‑1 (MQ=255)                                    tgGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGaa                                                                                                                               >  2:456984/1‑106 (MQ=255)                                    tgGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGaa                                                                                                                               <  1:456984/106‑1 (MQ=255)                                         cgcgcgCAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTaa                                                                                         <  1:792408/139‑1 (MQ=255)                                         cgcgcgCAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTaa                                                                                         <  1:288137/139‑1 (MQ=255)                                           cgcgcAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCAc                                                                                                           >  2:84849/1‑119 (MQ=255)                                           cgcgcAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCAc                                                                                                           <  1:84849/119‑1 (MQ=255)                                           cgcgcAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATg                                                                                       >  2:353516/1‑139 (MQ=255)                                             cgcAGTCATACATATGCAGGGCTAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGcc                                                                                     >  2:515268/1‑139 (MQ=255)                                              gcAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGccc                                                                                    <  2:87789/139‑1 (MQ=255)                                                    atacataTGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGcc                                                                              <  1:200249/139‑1 (MQ=255)                                                          atGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATc                                                                        >  2:211119/1‑139 (MQ=255)                                                            gCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAacac                                                                                                                            <  1:581982/85‑1 (MQ=255)                                                            gCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAacac                                                                                                                            >  2:581982/1‑85 (MQ=255)                                                            gCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCa                                                                                                  >  1:748002/1‑111 (MQ=255)                                                            gCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCa                                                                                                  <  2:748002/111‑1 (MQ=255)                                                            gCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCa                                                                                                  <  1:464150/111‑1 (MQ=255)                                                            gCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCa                                                                                                  >  2:464150/1‑111 (MQ=255)                                                            gCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCtt                                                                      >  1:640346/1‑139 (MQ=255)                                                            gCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCtt                                                                      >  1:893750/1‑139 (MQ=255)                                                            gCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCtt                                                                      >  2:145291/1‑139 (MQ=255)                                                               gggCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTAcg                                                                   <  2:893750/139‑1 (MQ=255)                                                                ggCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCGTTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCGTGATCTTAcgc                                                                  >  1:721141/1‑139 (MQ=255)                                                                ggCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTAcgc                                                                  >  2:756903/1‑139 (MQ=255)                                                                ggCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTAcgc                                                                  >  2:310776/1‑139 (MQ=255)                                                                ggCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTAcg                                                                   <  2:1077421/138‑1 (MQ=255)                                                                ggCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTAcg                                                                   >  1:1077421/1‑138 (MQ=255)                                                                  ccAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGaa                                                                                                                               <  2:372526/76‑1 (MQ=255)                                                                  ccAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGaa                                                                                                                               >  1:372526/1‑76 (MQ=255)                                                                  ccAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGa                                                                >  2:48478/1‑139 (MQ=255)                                                                  ccAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGa                                                                >  2:691935/1‑139 (MQ=255)                                                                     gcagTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAAt                                                                                        <  2:1099402/112‑1 (MQ=255)                                                                     gcagTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAAt                                                                                        >  1:1099402/1‑112 (MQ=255)                                                                                aCCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCg                                                  >  2:121116/1‑139 (MQ=255)                                                                                   gTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGgtg                                               >  2:488859/1‑139 (MQ=255)                                                                                   gTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGgtg                                               >  2:512553/1‑139 (MQ=255)                                                                                       ctTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGa                                           <  2:214464/139‑1 (MQ=255)                                                                                       ctTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGa                                           <  1:353516/139‑1 (MQ=255)                                                                                            tGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAgcgc                                      >  2:969731/1‑139 (MQ=255)                                                                                            tGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAgcgc                                      >  2:104589/1‑139 (MQ=255)                                                                                                gTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTAcgcg                                                                 <  1:56666/108‑1 (MQ=255)                                                                                                gTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTAcgcg                                                                 >  2:56666/1‑108 (MQ=255)                                                                                                  tCTTTGGGATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCtt                                                                            >  2:724325/1‑95 (MQ=255)                                                                                                  tCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCg                                                                                                         >  2:184452/1‑66 (MQ=255)                                                                                                  tCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCg                                                                                                         <  1:184452/66‑1 (MQ=255)                                                                                                  tCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCtt                                                                            <  1:724325/95‑1 (MQ=255)                                                                                                      tGGCATTCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGt                            <  1:691935/139‑1 (MQ=255)                                                                                                           ttCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAac                                                                                                                              >  1:414803/1‑36 (MQ=255)                                                                                                           ttCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAac                                                                                                                              <  2:414803/36‑1 (MQ=255)                                                                                                           ttCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTAcgcg                                                                 <  1:88703/97‑1 (MQ=255)                                                                                                           ttCGGTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTAcgcg                                                                 >  2:88703/1‑97 (MQ=255)                                                                                                              ggTGCTTTGTGGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCAcatcat                    <  1:512553/139‑1 (MQ=255)                                                                                                                     tgtgGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCAt                                                                                  >  2:562193/1‑70 (MQ=255)                                                                                                                     tgtgGAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCAt                                                                                  <  1:562193/70‑1 (MQ=255)                                                                                                                         gAAGTAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCTCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGAtg         <  2:611838/139‑1 (MQ=255)                                                                                                                             tAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGa                                           <  2:50839/101‑1 (MQ=255)                                                                                                                             tAAAGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGa                                           >  1:50839/1‑101 (MQ=255)                                                                                                                                aGATAGCCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTaaa  <  1:488859/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      GCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGTTTACGCACCGTCTCTTCATGCAGTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAA  >  NC_000913/697975‑698240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑