Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,170,493 (G)5→6 intergenic (‑119/+25) ldtC ← / ← mfd L,D‑transpeptidase linking Lpp to murein/transcription‑repair coupling factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,170,4881.G100.0% 68.7 / NA 19intergenic (‑114/+30)ldtC/mfdL,D‑transpeptidase linking Lpp to murein/transcription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (11/8);  total (11/8)

TTGTAATAAACAGATAAGCCTGAATTATGGATGGTGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGCGCAGAAACGCCCCCGATTTACCATTAAGCGATCGCGTTCTCTTCCAGTTCACGCATAAACTGGCGTACCCATTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAG  >  NC_000913/1170402‑1170610
                                                                                       |                                                                                                                          
ttGTAATAAACAGATAAGCCTGAATTATGGATGGTGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGGCGCAGAAACGccc                                                                                                          >  1:404447/1‑106 (MQ=255)
ttGTAATAAACAGATAAGCCTGAATTATGGATGGTGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGGCGCAGAAACGccc                                                                                                          <  2:404447/106‑1 (MQ=255)
ttGTAATAAACAGATAAGCCTGAATTATGGATGGTGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGGCGCAGAAACGCCCCCGAt                                                                                                     <  1:248668/111‑1 (MQ=255)
ttGTAATAAACAGATAAGCCTGAATTATGGATGGTGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGGCGCAGAAACGCCCCCGAt                                                                                                     >  2:248668/1‑111 (MQ=255)
ttGTAATAAACAGATAAGCCTGAATTATGGATGGTGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGGCGCAGAAACGCCCCCGATTTACCATTAAGCGATcgcg                                                                                  >  1:328322/1‑130 (MQ=255)
ttGTAATAAACAGATAAGCCTGAATTATGGATGGTGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGGCGCAGAAACGCCCCCGATTTACCATTAAGCGATcgcg                                                                                  <  2:328322/130‑1 (MQ=255)
             aTAAGCCTGAATTATGGATGGTGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGGCGCAGAAACGCCCCCGATTTACCATTAAGCGATCGCGTTCTCTTc                                                                          <  1:586249/125‑1 (MQ=255)
             aTAAGCCTGAATTATGGATGGTGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGGCGCAGAAACGCCCCCGATTTACCATTAAGCGATCGCGTTCTCTTc                                                                          >  2:586249/1‑125 (MQ=255)
                    tGAATTATGGATGGTGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGGCGCAGAAACGcccc                                                                                                         <  2:650817/87‑1 (MQ=255)
                    tGAATTATGGATGGTGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGGCGCAGAAACGcccc                                                                                                         >  1:650817/1‑87 (MQ=255)
                           tggatggTGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGGCGCAGAAACGCCCCCGATTTACCATTAAg                                                                                          <  2:805051/95‑1 (MQ=255)
                           tggatggTGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGGCGCAGAAACGCCCCCGATTTACCATTAAg                                                                                          >  1:805051/1‑95 (MQ=255)
                                  tGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGGCGCAGAAACGCCCCCGATTTACCATTAAGCGATcgcg                                                                                  <  1:658909/96‑1 (MQ=255)
                                  tGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGGCGCAGAAACGCCCCCGATTTACCATTAAGCGATcgcg                                                                                  >  2:658909/1‑96 (MQ=255)
                                        tgtCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATggg                                                                                                                         >  2:528236/1‑50 (MQ=255)
                                        tgtCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATCTTGCAGATcgg                                                                                                                         <  1:528236/50‑1 (MQ=255)
                                          tCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGGCGCAGAAACGCCCCCGATTTACCATTAAGCGATCGCGTTCTCTTCCAGTTCACGCATAAACTGGCGTACCCATTCGATACGCGTTTTc                               >  2:119369/1‑139 (MQ=255)
                                                  tGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGGCGCAGAAACGCCCCCGATTTACCATTAAGCGATCGCGTTCTCTTCCAGTTCACGCATAAACTGGCGTACCCATTCGATACGCGTTTTCCGctcact                       >  2:135447/1‑139 (MQ=255)
                                                    cAGGGAAGTGCAAATAATTTGTAAATGTTGCAGATGGGGGGCGCAGAAACGCCCCCGATTTACCATTAAGCGCTCGCGTTCTCTTCCAGTTCACGCATATACTGGCGTACCCATTCGATACGCGTTTTCCGctcactca                     >  2:554568/1‑139 (MQ=255)
                                                            tGCAAAGAATTTGTAAATGTTGCAGATGGGGGGCGCAGAAACGCCCCCGATTTACCATTAAGCGATCGCGTTCTCTTCCCGTTCACGCATAAACTGGCGTACCCATTCGATACGCGTTTTCCGCTCACTCAAATCCTGa             >  1:552675/1‑139 (MQ=255)
                                                                       tGTAAATGTTGCAGATGGGGGGCGCAGAAACGCCCCCGATTTACCATTAAGCGATCGCGTTCTCTTCCAGTTCACGCATAAACTGGCGTACCCATTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAg  <  2:552675/139‑1 (MQ=255)
                                                                                       |                                                                                                                          
TTGTAATAAACAGATAAGCCTGAATTATGGATGGTGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGCGCAGAAACGCCCCCGATTTACCATTAAGCGATCGCGTTCTCTTCCAGTTCACGCATAAACTGGCGTACCCATTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAG  >  NC_000913/1170402‑1170610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: